2. Membrane Transporter/Ion Channel Cell Cycle/DNA Damage
  3. VDAC RAD51
  4. DIDS sodium salt

DIDS sodium salt  (Synonyms: MDL101114ZA)

製品番号: HY-D0086 純度: 99.13%
COA 取扱説明書 Technical Support

DIDS sodium salt (MDL101114ZA) is a dual inhibitor of ABCA1 and VDAC1. DIDS also inhibits RAD51, inhibiting RAD51-mediated homologous pairing and strand exchange reactions. DIDS inhibits anion exchange and binding to red blood cell membranes, inhibits the activation of caspase-3 and -9, and can be used in cancer research.

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CAS 番号 : 67483-13-0

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
5 mg $29 在庫あり
10 mg $40 在庫あり
50 mg $80 在庫あり
100 mg $138 在庫あり
250 mg $240 在庫あり
500 mg $360 在庫あり
1 g   お問い合わせ  
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カスタマーレビュー

Based on 21 publication(s) in Google Scholar

Other Forms of DIDS sodium salt:

  • DIDS お問い合わせ

DIDS sodium salt 関連抗体

Top Publications Citing Use of Products

    DIDS sodium salt purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Nov 9;15(11):811.  [Abstract]

    H1299 cells were transfected with GFP or GFP-VDAC1 for 18 h and subsequently treated with 100 μM NSC15364 or 400 μM DIDS for 12 h.

    DIDS sodium salt purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Nov 9;15(11):811.  [Abstract]

    H1299 cells were incubated in a control or cysteine-deprived medium with or without DIDS for the indicated concentration for 24 h.

    DIDS sodium salt purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Nov 9;15(11):811.  [Abstract]

    H1299 cells were incubated in a control or cysteine-deprived medium with or without 400 μM DIDS for 12 h.

    DIDS sodium salt purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Nov 9;15(11):811.  [Abstract]

    H1299 cells were incubated in a control or cysteine-deprived medium with or without 100 μM NSC15364 or 400 μM DIDS for 12 h. A representative fluorescence microscopy image of MitoSOX red staining. The green fluorescence of MitoBright LT green shows the location of mitochondria (scale bar = 100 μm).

    DIDS sodium salt purchased from MedChemExpress. Usage Cited in: Autophagy. 2021 Nov;17(11):3592-3606.  [Abstract]

    Effect of DIDS (10 μM, 1 h-pretreatment) on CBD-induced glioma cell death. U251 or LN18 cells were pretreated with DIDS and then treated with CBD (30 μM) for 48 h. Cell viability was determined by the MTT assay.

    DIDS sodium salt purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2021 Nov;8(21):e2101936.  [Abstract]

    HCT116 cells were pretreated with DIDS (20 μM) for 1 h, followed by SR140333 treatment for 24 h.

    VDAC アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    VDAC1 VDAC2 VDAC3

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    製品説明

    DIDS sodium salt (MDL101114ZA) is a dual inhibitor of ABCA1 and VDAC1. DIDS also inhibits RAD51, inhibiting RAD51-mediated homologous pairing and strand exchange reactions. DIDS inhibits anion exchange and binding to red blood cell membranes, inhibits the activation of caspase-3 and -9, and can be used in cancer research[1][2][3][4].

    IC50 & Target

    VDAC1

     

    Cellular Effect
    Cell Line Type Value Description References
    PC-12 IC50
    1 3
    Compound: DIDS
    The compound was evaluated for inhibition of P2X purinoceptor in PC12 bladder cells
    The compound was evaluated for inhibition of P2X purinoceptor in PC12 bladder cells
    		[PMID: 12213051]
    PC-12 IC50
    1 3
    Compound: DIDS
    The compound was evaluated for inhibition of P2X purinoceptor in PC12 bladder cells
    The compound was evaluated for inhibition of P2X purinoceptor in PC12 bladder cells
    		[PMID: 12213051]
    体外実験

    DIDS sodium salt (0-10 μM) inhibits RAD51-mediated strand exchange[1].
    DIDS sodium salt (0-20 μM) inhibits DNA binding by RAD51[1].
    DIDS sodium salt (10 μM; 0-60 min) stimulates the ATP hydrolyzing activity of RAD51 in the absence of DNA[1].
    DIDS sodium salt (50-400 μM) prevents effect on ALA-SDT-induced cell death, while dose at 50 μM has no inhibition effect, and dose at 400 μM insifnificantly decreases the cell viability[2].
    DIDS sodium salt (100 μM) clearly inhibits caspase-3 and caspase-9 activation[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    DIDS sodium salt 関連抗体
    臨床実験
    分子量

    498.48

    分子式

    C16H8N2Na2O6S4
    CAS 番号
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    O=S(C1=CC(N=C=S)=CC=C1/C=C/C2=CC=C(N=C=S)C=C2S(=O)(O[Na])=O)(O[Na])=O
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    -20°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
    溶剤 & 溶解度
    体外: 

    DMSO : 125 mg/mL (250.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.0061 mL 10.0305 mL 20.0610 mL
    5 mM 0.4012 mL 2.0061 mL 4.0122 mL
    10 mM 0.2006 mL 1.0030 mL 2.0061 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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    一般には略語で表示されます：C1V1 = C2V2

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    純度とドキュメンテーション

    純度: 99.13%

    COA (252 KB) HNMR (271 KB) LCMS (266 KB)

    取扱説明書 (2659 KB)
    参考文献
    細胞実験

    Cells (5×105 cells/mL) are seeded in the 35-mm Petri dishes and cultured for 72 h at 37°C. Cells are pre-incubated with ALA, and then DIDS of diverse concentrations (0-200 μM) is added before exposed to ultrasound. Cell apoptosis is detected. Briefly, seeded cells (5×105 cells/mL) are pre-treated with test compounds at the indicated concentrations for 1 h (DIDS and NAC), 1.5 h (BAPTA) or with siRNA and exposed to ultrasound. Six hours after ALA-SDT treatment, cells are collected[2].

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.0061 mL 10.0305 mL 20.0610 mL 50.1525 mL
    5 mM 0.4012 mL 2.0061 mL 4.0122 mL 10.0305 mL
    10 mM 0.2006 mL 1.0030 mL 2.0061 mL 5.0152 mL
    15 mM 0.1337 mL 0.6687 mL 1.3374 mL 3.3435 mL
    20 mM 0.1003 mL 0.5015 mL 1.0030 mL 2.5076 mL
    25 mM 0.0802 mL 0.4012 mL 0.8024 mL 2.0061 mL
    30 mM 0.0669 mL 0.3343 mL 0.6687 mL 1.6717 mL
    40 mM 0.0502 mL 0.2508 mL 0.5015 mL 1.2538 mL
    50 mM 0.0401 mL 0.2006 mL 0.4012 mL 1.0030 mL
    60 mM 0.0334 mL 0.1672 mL 0.3343 mL 0.8359 mL
    80 mM 0.0251 mL 0.1254 mL 0.2508 mL 0.6269 mL
    100 mM 0.0201 mL 0.1003 mL 0.2006 mL 0.5015 mL
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    DIDS sodium salt Related Classifications

    • Molarity Calculator

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    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    DIDS67483-13-0MDL101114ZAVDACRAD51Voltage-dependent anion channelhomologous pairinganion exchangered blood cell membraneInhibitorinhibitorinhibit

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    製品番号:
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