DIDS
Based on 22 publication(s) in Google Scholar
DIDS is a dual inhibitor of ABCA1 and VDAC1. DIDS also inhibits RAD51, inhibiting RAD51-mediated homologous pairing and strand exchange reactions. DIDS inhibits anion exchange and binding to red blood cell membranes, inhibits the activation of caspase-3 and -9, and can be used in cancer research.
For research use only. We do not sell to patients.
- CAS No.: 53005-05-3
- Formula: C16H10N2O6S4
- Molecular Weight:454.52
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Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
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- References
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Publications Citing Use of MedChemExpress (MCE) DIDS
More- Autophagy. 2021 Nov;17(11):3592-3606. [Abstract]
- Adv Sci (Weinh). 2021 Nov;8(21):e2101936. [Abstract]
- Cell Death Dis. 2024 Nov 9;15(11):811. [Abstract]
- Phytomedicine. 2024 Jul 25:130:155785. [Abstract]
- Cell Death Discov. 2023 Jul 8;9(1):234. [Abstract]
- Proc Natl Acad Sci U S A. 2025 Oct 7;122(40):e2502841122. [Abstract]
- Br J Pharmacol. 2025 Aug;182(16):3923-3951. [Abstract]
- Virulence. 2025 Dec;16(1):2490208. [Abstract]
- CNS Neurosci Ther. 2025 Apr;31(4):e70410. [Abstract]
- Life Sci. 2020 Oct 15:259:118390. [Abstract]
- Front Pharmacol. 2023 Jun 26:14:1191692. [Abstract]
- Cancer Sci. 2020 Nov;111(11):4288-4302. [Abstract]
- Cancers (Basel). 2025 Dec 27;18(1):92. [Abstract]
- J Cell Mol Med. 2021 Jun;25(11):5238-5249. [Abstract]
- J Virol. 2026 Mar 24;100(3):e0220025. [Abstract]
- FEBS Open Bio. 2022 Feb;12(2):516-522. [Abstract]
- Biochem Biophys Res Commun. 2021 Jun 30:560:52-58. [Abstract]
- bioRxiv. 2026 Apr 7:2026.04.04.716514. [Abstract]
- Sci Total Environ. 2024 Oct 10:946:174246. [Abstract]
- bioRxiv. 2024 July 10.
- SSRN. 2023 Nov 14.
- bioRxiv. 2023 Jul 3.
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Cell Proliferation/Viability Assay
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Cell Proliferation/Viability Assay
Biological Activity
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VDAC1 |
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Cell Line
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Type | Value | Description | References |
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| Erythrocyte | IC50 |
31 μM
Compound: DIDS
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Inhibition of erythrocyte anion transport protein
Inhibition of erythrocyte anion transport protein
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[PMID: 18078758] |
DIDS (0-10 μM) inhibits RAD51-mediated strand exchange[1].
DIDS (0-20 μM) inhibits DNA binding by RAD51[1].
DIDS (10 μM; 0-60 min) stimulates the ATP hydrolyzing activity of RAD51 in the absence of DNA[1].
DIDS (50-400 μM) prevents effect on ALA-SDT-induced cell death, while dose at 50 μM has no inhibition effect, and dose at 400 μM insifnificantly decreases the cell viability[2].
DIDS (100 μM) clearly inhibits caspase-3 and caspase-9 activation[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 53005-05-3
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Molecular Weight 454.52
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Formula C16H10N2O6S4
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SMILES
O=S(C1=CC(N=C=S)=CC=C1/C=C/C2=CC=C(C=C2S(=O)(O)=O)N=C=S)(O)=O
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Synonyms
MDL101114ZA free base
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (22)
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Journal Impact Factor
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Most Recent
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Autophagy
Cannabidiol inhibits human glioma by induction of lethal mitophagy through activating TRPV4. [Abstract]2021 Nov;17(11):3592-3606. PMID: 33629929
DIDS purchased from MedChemExpress. Usage Cited in: Autophagy. 2021 Nov;17(11):3592-3606. [Abstract]
Effect of DIDS (10 μM, 1 h-pretreatment) on CBD-induced glioma cell death. U251 or LN18 cells were pretreated with DIDS and then treated with CBD (30 μM) for 48 h. Cell viability was determined by the MTT assay.
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Adv Sci (Weinh)
A Novel Mechanism of Endoplasmic Reticulum Stress- and c-Myc-Degradation-Mediated Therapeutic Benefits of Antineurokinin-1 Receptor Drugs in Colorectal Cancer. [Abstract]2021 Nov;8(21):e2101936. PMID: 34605226
DIDS purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2021 Nov;8(21):e2101936. [Abstract]
HCT116 cells were pretreated with DIDS (20 μM) for 1 h, followed by SR140333 treatment for 24 h.
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Cell Death Dis
Inhibition of VDAC1 oligomerization blocks cysteine deprivation-induced ferroptosis via mitochondrial ROS suppression. [Abstract]2024 Nov 9;15(11):811. PMID: 39521767
DIDS purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Nov 9;15(11):811. [Abstract]
H1299 cells were transfected with GFP or GFP-VDAC1 for 18 h and subsequently treated with 100 μM NSC15364 or 400 μM DIDS for 12 h.
DIDS purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Nov 9;15(11):811. [Abstract]
H1299 cells were incubated in a control or cysteine-deprived medium with or without DIDS for the indicated concentration for 24 h.
DIDS purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Nov 9;15(11):811. [Abstract]
H1299 cells were incubated in a control or cysteine-deprived medium with or without 400 μM DIDS for 12 h.
DIDS purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Nov 9;15(11):811. [Abstract]
H1299 cells were incubated in a control or cysteine-deprived medium with or without 100 μM NSC15364 or 400 μM DIDS for 12 h. A representative fluorescence microscopy image of MitoSOX red staining. The green fluorescence of MitoBright LT green shows the location of mitochondria (scale bar = 100 μm).
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Phytomedicine
Ginseng total saponin improves red blood cell oxidative stress injury by regulating tyrosine phosphorylation and glycolysis in red blood cells. [Abstract]2024 Jul 25:130:155785. PMID: 38823342 -
Cell Death Discov
SCP2 mediates the transport of lipid hydroperoxides to mitochondria in chondrocyte ferroptosis. [Abstract]2023 Jul 8;9(1):234. PMID: 37422468 -
Proc Natl Acad Sci U S A
Mitochondrial ROS triggers mitophagy through activating the DNA damage response signaling pathway. [Abstract]2025 Oct 7;122(40):e2502841122. PMID: 41026812 -
Br J Pharmacol
A mitochondria-targeting and G-quadruplex structure-binding ligand inducing calcium overload and ferroptosis in human cancer cells. [Abstract]2025 Aug;182(16):3923-3951. PMID: 40344208 -
Virulence
2025 Dec;16(1):2490208. PMID: 40202859 -
CNS Neurosci Ther
VDAC1 Inhibition Protects Against Noise-Induced Hearing Loss via the PINK1/Parkin Pathway. [Abstract]2025 Apr;31(4):e70410. PMID: 40285415 -
Life Sci
2020 Oct 15:259:118390. PMID: 32896556 -
Front Pharmacol
HECT, UBA and WWE domain containing 1 represses cholesterol efflux during CD4+ T cell activation in Sjögren's syndrome. [Abstract]2023 Jun 26:14:1191692. PMID: 37435494 -
Cancer Sci
System biology analysis reveals the role of voltage-dependent anion channel in mitochondrial dysfunction during non-alcoholic fatty liver disease progression into hepatocellular carcinoma. [Abstract]2020 Nov;111(11):4288-4302. PMID: 32945042 -
Cancers (Basel)
Synthesis and Biological Evaluation of a Caffeic Acid Phenethyl Ester Derivatives as Anti-Hepatocellular Carcinoma Agents via Inhibition of Mitochondrial Respiration and Disruption of Cellular Metabolism. [Abstract]2025 Dec 27;18(1):92. PMID: 41514605 -
J Cell Mol Med
Photobiomodulation therapy promotes the ATP-binding cassette transporter A1-dependent cholesterol efflux in macrophage to ameliorate atherosclerosis. [Abstract]2021 Jun;25(11):5238-5249. PMID: 33951300 -
J Virol
DIDS modulates VDAC1 oligomerization to suppress intrinsic apoptosis and attenuates in vitro and in vivo RSV infection. [Abstract]2026 Mar 24;100(3):e0220025. PMID: 41670372 -
FEBS Open Bio
VDAC1 oligomerization may enhance DDP-induced hepatocyte apoptosis by exacerbating oxidative stress and mitochondrial DNA damage. [Abstract]2022 Feb;12(2):516-522. PMID: 34967508 -
Biochem Biophys Res Commun
VDAC1 as a target in cisplatin anti-tumor activity through promoting mitochondria fusion. [Abstract]2021 Jun 30:560:52-58. PMID: 33971568 -
bioRxiv
2026 Apr 7:2026.04.04.716514. PMID: 41993559 -
Sci Total Environ
Voltage-dependent anion channel 1 mediates mitochondrial fission and glucose metabolic reprogramming in response to ionizing radiation. [Abstract]2024 Oct 10:946:174246. PMID: 38955266 -
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Purity & Documentation
References
[1]. Ishida T, et, al. DIDS, a chemical compound that inhibits RAD51-mediated homologous pairing and strand exchange. Nucleic Acids Res. 2009 Jun;37(10):3367-76. [Content Brief]
[2]. Lepke S, et, al. A study of the relationship between inhibition of anion exchange and binding to the red blood cell membrane of 4,4'-diisothiocyano stilbene-2,2'-disulfonic acid (DIDS) and its dihydro derivative (H2DIDS). J Membr Biol. 1976 Oct 20;29(1-2):147-77. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
DIDS Related Classifications
HY-121693 Related Classifications
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