DIDS sodium salt (Synonyms: MDL101114ZA)

Name: DIDS sodium salt
Brand: MedChemExpress
SKU: HY-D0086
Rating: 5.0 (21 reviews)

Cat. No.: HY-D0086 Purity: 99.13%: Data Sheet
COA

SDS
Handling Instructions
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DIDS sodium salt (MDL101114ZA) is a dual inhibitor of ABCA1 and VDAC1. DIDS also inhibits RAD51, inhibiting RAD51-mediated homologous pairing and strand exchange reactions. DIDS inhibits anion exchange and binding to red blood cell membranes, inhibits the activation of caspase-3 and -9, and can be used in cancer research.

For research use only. We do not sell to patients.

CAS No. : 67483-13-0

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5 mg	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 21 publication(s) in Google Scholar

Other Forms of DIDS sodium salt:

DIDS Get quote

21 Publications Citing Use of MCE DIDS sodium salt

Flow Cytometry

Cell Proliferation/Viability Assay

: DIDS sodium salt purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Nov 9;15(11):811. [Abstract]; H1299 cells were transfected with GFP or GFP-VDAC1 for 18 h and subsequently treated with 100 μM NSC15364 or 400 μM DIDS for 12 h.

: DIDS sodium salt purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Nov 9;15(11):811. [Abstract]; H1299 cells were incubated in a control or cysteine-deprived medium with or without DIDS for the indicated concentration for 24 h.

: DIDS sodium salt purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Nov 9;15(11):811. [Abstract]; H1299 cells were incubated in a control or cysteine-deprived medium with or without 400 μM DIDS for 12 h.

: DIDS sodium salt purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Nov 9;15(11):811. [Abstract]; H1299 cells were incubated in a control or cysteine-deprived medium with or without 100 μM NSC15364 or 400 μM DIDS for 12 h. A representative fluorescence microscopy image of MitoSOX red staining. The green fluorescence of MitoBright LT green shows the location of mitochondria (scale bar = 100 μm).

: DIDS sodium salt purchased from MedChemExpress. Usage Cited in: Autophagy. 2021 Nov;17(11):3592-3606. [Abstract]; Effect of various ion channel inhibitors on CBD-induced glioma cell death. U251 or LN18 cells were pretreated with rimonabant (1 μM), SR144528 (1 μM), SEA0400 (10 μM), DIDS (10 μM), CID16020046 (1 μM), SKF96365 (1 μM), RuR (1 μM), mibefradil (2.5 uM), capsazepine (1 μM), or GSK2193874 (5 μM) for 1 h and then treated with CBD (30 μM) for 48 h. Cell viability was determined by the MTT assay.

: DIDS sodium salt purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2021 Nov;8(21):e2101936. [Abstract]; HCT116 cells were pretreated with DIDS (20 μM) for 1 h, followed by SR140333 treatment for 24 h.

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Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

VDAC1

Cellular Effect

Cell Line	Type	Value	Description	References
PC-12	IC₅₀	1 μM Compound: DIDS	The compound was evaluated for inhibition of P2X purinoceptor in PC12 bladder cells The compound was evaluated for inhibition of P2X purinoceptor in PC12 bladder cells	[PMID: 12213051]

In Vitro

DIDS sodium salt (0-10 μM) inhibits RAD51-mediated strand exchange^[1].
DIDS sodium salt (0-20 μM) inhibits DNA binding by RAD51^[1].
DIDS sodium salt (10 μM; 0-60 min) stimulates the ATP hydrolyzing activity of RAD51 in the absence of DNA^[1].
DIDS sodium salt (50-400 μM) prevents effect on ALA-SDT-induced cell death, while dose at 50 μM has no inhibition effect, and dose at 400 μM insifnificantly decreases the cell viability^[2].
DIDS sodium salt (100 μM) clearly inhibits caspase-3 and caspase-9 activation^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

498.48

Formula

C₁₆H₈N₂Na₂O₆S₄

CAS No.

67483-13-0

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=S(C1=CC(N=C=S)=CC=C1/C=C/C2=CC=C(N=C=S)C=C2S(=O)(O[Na])=O)(O[Na])=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (250.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0061 mL	10.0305 mL	20.0610 mL
5 mM	0.4012 mL	2.0061 mL	4.0122 mL
10 mM	0.2006 mL	1.0030 mL	2.0061 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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Concentration _(final)

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.13%

Select Batch:

Data Sheet (277 KB) SDS (585 KB)

COA (252 KB) HNMR (271 KB) LCMS (266 KB)

Handling Instructions (2659 KB)

References

[1]. Chen H, et al. Inhibition of VDAC1 prevents Ca²⁺-mediated oxidative stress and apoptosis induced by 5-aminolevulinic acid mediated sonodynamic therapy in THP-1 macrophages. Apoptosis. 2014 Dec;19(12):1712-26. [Content Brief]

[2]. Tsou CY, et al. Activation of soluble guanylyl cyclase prevents foam cell formation and atherosclerosis. Acta Physiol (Oxf). 2014 Apr;210(4):799-810. [Content Brief]

Cell Assay

Cells (5×10⁵ cells/mL) are seeded in the 35-mm Petri dishes and cultured for 72 h at 37°C. Cells are pre-incubated with ALA, and then DIDS of diverse concentrations (0-200 μM) is added before exposed to ultrasound. Cell apoptosis is detected. Briefly, seeded cells (5×10⁵ cells/mL) are pre-treated with test compounds at the indicated concentrations for 1 h (DIDS and NAC), 1.5 h (BAPTA) or with siRNA and exposed to ultrasound. Six hours after ALA-SDT treatment, cells are collected^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Chen H, et al. Inhibition of VDAC1 prevents Ca²⁺-mediated oxidative stress and apoptosis induced by 5-aminolevulinic acid mediated sonodynamic therapy in THP-1 macrophages. Apoptosis. 2014 Dec;19(12):1712-26. [Content Brief]

[2]. Tsou CY, et al. Activation of soluble guanylyl cyclase prevents foam cell formation and atherosclerosis. Acta Physiol (Oxf). 2014 Apr;210(4):799-810. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.0061 mL	10.0305 mL	20.0610 mL	50.1525 mL
	5 mM	0.4012 mL	2.0061 mL	4.0122 mL	10.0305 mL
	10 mM	0.2006 mL	1.0030 mL	2.0061 mL	5.0152 mL
	15 mM	0.1337 mL	0.6687 mL	1.3374 mL	3.3435 mL
	20 mM	0.1003 mL	0.5015 mL	1.0030 mL	2.5076 mL
	25 mM	0.0802 mL	0.4012 mL	0.8024 mL	2.0061 mL
	30 mM	0.0669 mL	0.3343 mL	0.6687 mL	1.6717 mL
	40 mM	0.0502 mL	0.2508 mL	0.5015 mL	1.2538 mL
	50 mM	0.0401 mL	0.2006 mL	0.4012 mL	1.0030 mL
	60 mM	0.0334 mL	0.1672 mL	0.3343 mL	0.8359 mL
	80 mM	0.0251 mL	0.1254 mL	0.2508 mL	0.6269 mL
	100 mM	0.0201 mL	0.1003 mL	0.2006 mL	0.5015 mL

DIDS sodium salt Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DIDS67483-13-0MDL101114ZAVDACRAD51Voltage-dependent anion channelhomologous pairinganion exchangered blood cell membraneInhibitorinhibitorinhibit

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DIDS sodium salt (Synonyms: MDL101114ZA)

Customer Review

Other Forms of DIDS sodium salt:

DIDS sodium salt Related Antibodies

21 Publications Citing Use of MCE DIDS sodium salt

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•Related Small Molecules:

•Related Proteins:

View All VDAC Isoform Specific Products:

Biological Activity

Protocol

Purity & Documentation

References

Customer Review

DIDS sodium salt Related Antibodies

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Complete Stock Solution Preparation Table

DIDS sodium salt Related Classifications

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