DIDS sodium salt
Based on 22 publication(s) in Google Scholar
DIDS sodium salt (MDL101114ZA) is a dual inhibitor of ABCA1 and VDAC1. DIDS also inhibits RAD51, inhibiting RAD51-mediated homologous pairing and strand exchange reactions. DIDS inhibits anion exchange and binding to red blood cell membranes, inhibits the activation of caspase-3 and -9, and can be used in cancer research.
For research use only. We do not sell to patients.
- Purity: 99.13%
- CAS No.: 67483-13-0
- Formula: C16H8N2Na2O6S4
- Molecular Weight:498.48
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Storage:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) DIDS sodium salt
More- Autophagy. 2021 Nov;17(11):3592-3606. [Abstract]
- Adv Sci (Weinh). 2021 Nov;8(21):e2101936. [Abstract]
- Cell Death Dis. 2024 Nov 9;15(11):811. [Abstract]
- Proc Natl Acad Sci U S A. 2025 Oct 7;122(40):e2502841122. [Abstract]
- Phytomedicine. 2024 May 28:130:155785. [Abstract]
- Sci Total Environ. 2024 Jun 30:946:174246. [Abstract]
- Br J Pharmacol. 2025 Aug;182(16):3923-3951. [Abstract]
- Cell Death Discov. 2023 Jul 8;9(1):234. [Abstract]
- Virulence. 2025 Dec;16(1):2490208. [Abstract]
- Life Sci. 2020 Oct 15;259:118390. [Abstract]
- CNS Neurosci Ther. 2025 Apr;31(4):e70410. [Abstract]
- Front Pharmacol. 2023 Jun 26:14:1191692. [Abstract]
- Cancers (Basel). 2025 Dec 27;18(1):92. [Abstract]
- Cancer Sci. 2020 Nov;111(11):4288-4302. [Abstract]
- J Cell Mol Med. 2021 Jun;25(11):5238-5249. [Abstract]
- J Virol. 2026 Mar 24;100(3):e0220025. [Abstract]
- FEBS Open Bio. 2022 Feb;12(2):516-522. [Abstract]
- Biochem Biophys Res Commun. 2021 Jun 30:560:52-58. [Abstract]
- bioRxiv. 2026 Apr 7:2026.04.04.716514. [Abstract]
- bioRxiv. 2024 July 10.
- SSRN. 2023 Nov 14.
- bioRxiv. 2023 Jul 3.
Customer Validation & Images
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WB
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Cell Proliferation/Viability Assay
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Flow Cytometry
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IF
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Cell Proliferation/Viability Assay
Biological Activity
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VDAC1 |
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Cell Line
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Type | Value | Description | References |
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| PC-12 | IC50 |
1 μM
Compound: DIDS
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The compound was evaluated for inhibition of P2X purinoceptor in PC12 bladder cells
The compound was evaluated for inhibition of P2X purinoceptor in PC12 bladder cells
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[PMID: 12213051] |
DIDS sodium salt (0-10 μM) inhibits RAD51-mediated strand exchange[1].
DIDS sodium salt (0-20 μM) inhibits DNA binding by RAD51[1].
DIDS sodium salt (10 μM; 0-60 min) stimulates the ATP hydrolyzing activity of RAD51 in the absence of DNA[1].
DIDS sodium salt (50-400 μM) prevents effect on ALA-SDT-induced cell death, while dose at 50 μM has no inhibition effect, and dose at 400 μM insifnificantly decreases the cell viability[2].
DIDS sodium salt (100 μM) clearly inhibits caspase-3 and caspase-9 activation[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 67483-13-0
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Appearance Solid
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Molecular Weight 498.48
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Formula C16H8N2Na2O6S4
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Color Light yellow to yellow
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SMILES
O=S(C1=CC(N=C=S)=CC=C1/C=C/C2=CC=C(N=C=S)C=C2S(=O)(O[Na])=O)(O[Na])=O
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Synonyms
MDL101114ZA
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (22)
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Journal Impact Factor
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Most Recent
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Autophagy
Cannabidiol inhibits human glioma by induction of lethal mitophagy through activating TRPV4. [Abstract]2021 Nov;17(11):3592-3606. PMID: 33629929
DIDS sodium salt purchased from MedChemExpress. Usage Cited in: Autophagy. 2021 Nov;17(11):3592-3606. [Abstract]
Effect of DIDS (10 μM, 1 h-pretreatment) on CBD-induced glioma cell death. U251 or LN18 cells were pretreated with DIDS and then treated with CBD (30 μM) for 48 h. Cell viability was determined by the MTT assay.
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Adv Sci (Weinh)
A Novel Mechanism of Endoplasmic Reticulum Stress- and c-Myc-Degradation-Mediated Therapeutic Benefits of Antineurokinin-1 Receptor Drugs in Colorectal Cancer. [Abstract]2021 Nov;8(21):e2101936. PMID: 34605226
DIDS sodium salt purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2021 Nov;8(21):e2101936. [Abstract]
HCT116 cells were pretreated with DIDS (20 μM) for 1 h, followed by SR140333 treatment for 24 h.
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Cell Death Dis
Inhibition of VDAC1 oligomerization blocks cysteine deprivation-induced ferroptosis via mitochondrial ROS suppression. [Abstract]2024 Nov 9;15(11):811. PMID: 39521767
DIDS sodium salt purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Nov 9;15(11):811. [Abstract]
H1299 cells were transfected with GFP or GFP-VDAC1 for 18 h and subsequently treated with 100 μM NSC15364 or 400 μM DIDS for 12 h.
DIDS sodium salt purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Nov 9;15(11):811. [Abstract]
H1299 cells were incubated in a control or cysteine-deprived medium with or without DIDS for the indicated concentration for 24 h.
DIDS sodium salt purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Nov 9;15(11):811. [Abstract]
H1299 cells were incubated in a control or cysteine-deprived medium with or without 400 μM DIDS for 12 h.
DIDS sodium salt purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Nov 9;15(11):811. [Abstract]
H1299 cells were incubated in a control or cysteine-deprived medium with or without 100 μM NSC15364 or 400 μM DIDS for 12 h. A representative fluorescence microscopy image of MitoSOX red staining. The green fluorescence of MitoBright LT green shows the location of mitochondria (scale bar = 100 μm).
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Proc Natl Acad Sci U S A
Mitochondrial ROS triggers mitophagy through activating the DNA damage response signaling pathway. [Abstract]2025 Oct 7;122(40):e2502841122. PMID: 41026812 -
Phytomedicine
Ginseng total saponin improves red blood cell oxidative stress injury by regulating tyrosine phosphorylation and glycolysis in red blood cells. [Abstract]2024 May 28:130:155785. PMID: 38823342 -
Sci Total Environ
Voltage-dependent anion channel 1 mediates mitochondrial fission and glucose metabolic reprogramming in response to ionizing radiation. [Abstract]2024 Jun 30:946:174246. PMID: 38955266 -
Br J Pharmacol
A mitochondria-targeting and G-quadruplex structure-binding ligand inducing calcium overload and ferroptosis in human cancer cells. [Abstract]2025 Aug;182(16):3923-3951. PMID: 40344208 -
Cell Death Discov
SCP2 mediates the transport of lipid hydroperoxides to mitochondria in chondrocyte ferroptosis. [Abstract]2023 Jul 8;9(1):234. PMID: 37422468 -
Virulence
2025 Dec;16(1):2490208. PMID: 40202859 -
Life Sci
2020 Oct 15;259:118390. PMID: 32896556 -
CNS Neurosci Ther
VDAC1 Inhibition Protects Against Noise-Induced Hearing Loss via the PINK1/Parkin Pathway. [Abstract]2025 Apr;31(4):e70410. PMID: 40285415 -
Front Pharmacol
HECT, UBA and WWE domain containing 1 represses cholesterol efflux during CD4+ T cell activation in Sjögren's syndrome. [Abstract]2023 Jun 26:14:1191692. PMID: 37435494 -
Cancers (Basel)
Synthesis and Biological Evaluation of a Caffeic Acid Phenethyl Ester Derivatives as Anti-Hepatocellular Carcinoma Agents via Inhibition of Mitochondrial Respiration and Disruption of Cellular Metabolism. [Abstract]2025 Dec 27;18(1):92. PMID: 41514605 -
Cancer Sci
System biology analysis reveals the role of voltage-dependent anion channel in mitochondrial dysfunction during non-alcoholic fatty liver disease progression into hepatocellular carcinoma. [Abstract]2020 Nov;111(11):4288-4302. PMID: 32945042 -
J Cell Mol Med
Photobiomodulation therapy promotes the ATP-binding cassette transporter A1-dependent cholesterol efflux in macrophage to ameliorate atherosclerosis. [Abstract]2021 Jun;25(11):5238-5249. PMID: 33951300 -
J Virol
DIDS modulates VDAC1 oligomerization to suppress intrinsic apoptosis and attenuates in vitro and in vivo RSV infection. [Abstract]2026 Mar 24;100(3):e0220025. PMID: 41670372 -
FEBS Open Bio
VDAC1 oligomerization may enhance DDP-induced hepatocyte apoptosis by exacerbating oxidative stress and mitochondrial DNA damage. [Abstract]2022 Feb;12(2):516-522. PMID: 34967508 -
Biochem Biophys Res Commun
VDAC1 as a target in cisplatin anti-tumor activity through promoting mitochondria fusion. [Abstract]2021 Jun 30:560:52-58. PMID: 33971568 -
bioRxiv
2026 Apr 7:2026.04.04.716514. PMID: 41993559 -
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Solvent & Solubility
DMSO : 125 mg/mL (250.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
Cells (5×105 cells/mL) are seeded in the 35-mm Petri dishes and cultured for 72 h at 37°C. Cells are pre-incubated with ALA, and then DIDS of diverse concentrations (0-200 μM) is added before exposed to ultrasound. Cell apoptosis is detected. Briefly, seeded cells (5×105 cells/mL) are pre-treated with test compounds at the indicated concentrations for 1 h (DIDS and NAC), 1.5 h (BAPTA) or with siRNA and exposed to ultrasound. Six hours after ALA-SDT treatment, cells are collected[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (277 KB)
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SDS (585 KB)
- English - EN (585 KB)
- Français - FR (585 KB)
- Deutsch - DE (585 KB)
- Norwegian - NO (585 KB)
- Español - ES (585 KB)
- Swedish - SV (585 KB)
- Italian - IT (585 KB)
- Korean - KR (585 KB)
- Portuguese - PT (585 KB)
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Handling Instructions (2659 KB)
References
[1]. Chen H, et al. Inhibition of VDAC1 prevents Ca²⁺-mediated oxidative stress and apoptosis induced by 5-aminolevulinic acid mediated sonodynamic therapy in THP-1 macrophages. Apoptosis. 2014 Dec;19(12):1712-26. [Content Brief]
[2]. Tsou CY, et al. Activation of soluble guanylyl cyclase prevents foam cell formation and atherosclerosis. Acta Physiol (Oxf). 2014 Apr;210(4):799-810. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.0061 mL | 10.0305 mL | 20.0610 mL | 50.1525 mL |
| 5 mM | 0.4012 mL | 2.0061 mL | 4.0122 mL | 10.0305 mL | |
| 10 mM | 0.2006 mL | 1.0030 mL | 2.0061 mL | 5.0152 mL | |
| 15 mM | 0.1337 mL | 0.6687 mL | 1.3374 mL | 3.3435 mL | |
| 20 mM | 0.1003 mL | 0.5015 mL | 1.0030 mL | 2.5076 mL | |
| 25 mM | 0.0802 mL | 0.4012 mL | 0.8024 mL | 2.0061 mL | |
| 30 mM | 0.0669 mL | 0.3343 mL | 0.6687 mL | 1.6717 mL | |
| 40 mM | 0.0502 mL | 0.2508 mL | 0.5015 mL | 1.2538 mL | |
| 50 mM | 0.0401 mL | 0.2006 mL | 0.4012 mL | 1.0030 mL | |
| 60 mM | 0.0334 mL | 0.1672 mL | 0.3343 mL | 0.8359 mL | |
| 80 mM | 0.0251 mL | 0.1254 mL | 0.2508 mL | 0.6269 mL | |
| 100 mM | 0.0201 mL | 0.1003 mL | 0.2006 mL | 0.5015 mL |
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