1. NF-κB
    Immunology/Inflammation
    Anti-infection
    Autophagy
    Metabolic Enzyme/Protease
  2. Keap1-Nrf2
    Reactive Oxygen Species
    HIV
    Autophagy
    Endogenous Metabolite
  3. Dimethyl fumarate

Dimethyl fumarate (Synonyms: DMF)

Cat. No.: HY-17363 Purity: ≥98.0%
Handling Instructions

Dimethyl fumarate (DMF) is an orally active and brain-penetrant Nrf2 activator and induces upregulation of antioxidant gene expression. Dimethyl fumarate induces necroptosis in colon cancer cells through GSH depletion/ROS increase/MAPKs activation pathway, and also induces cell autophagy. Dimethyl fumarate can be used for multiple sclerosis research.

For research use only. We do not sell to patients.

Dimethyl fumarate Chemical Structure

Dimethyl fumarate Chemical Structure

CAS No. : 624-49-7

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply Now  
10 mM * 1 mL in DMSO USD 55 In-stock
Estimated Time of Arrival: December 31
500 mg USD 50 In-stock
Estimated Time of Arrival: December 31
5 g USD 66 In-stock
Estimated Time of Arrival: December 31
10 g   Get quote  
50 g   Get quote  

* Please select Quantity before adding items.

Customer Review

Based on 7 publication(s) in Google Scholar

Other Forms of Dimethyl fumarate:

Top Publications Citing Use of Products

    Dimethyl fumarate purchased from MCE. Usage Cited in: J Mol Endocrinol. 2018 Oct 15;61(4):163-172.

    Keratinocytes are subjected to normal glucose (NG), H2O2, or high glucose (HG) in the presence or absence of DMF, to measure the levels of NRF2 protein.

    Dimethyl fumarate purchased from MCE. Usage Cited in: Aging (Albany NY). 2018 Aug 16;10(8):2016-2036.

    Relative changes in the expression of Nrf2, pNrf2 and HO-1 after treatment with D-gal alone or combined with DMF or lycopene.
    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Dimethyl fumarate (DMF) is an orally active and brain-penetrant Nrf2 activator and induces upregulation of antioxidant gene expression. Dimethyl fumarate induces necroptosis in colon cancer cells through GSH depletion/ROS increase/MAPKs activation pathway, and also induces cell autophagy. Dimethyl fumarate can be used for multiple sclerosis research[1][2].

    IC50 & Target

    Human Endogenous Metabolite

     

    In Vitro

    Dimethyl fumarate (DMF; 20-200 μM; 24 hours) treatment dose-dependently reduces the viability of SGC-7901, HT29, HCT116 and CT26 cancer cells[1].
    Dimethyl fumarate (DMF; 100 μM; 3-24 hours) significantly activates JNK, p38 and ERK in CT26 cells[1].
    Dimethyl fumarate induces necroptosis in colon cancer cells and the mechanism involves GSH depletion, an increase in ROS and activation of MAPKs-mediated signalling[1].
    Dimethyl fumarate inhibits dendritic cell (DC) maturation by reducing inflammatory cytokine production (IL-12 and IL-6) and the expression of MHC class II, CD80, and CD86. Dimethyl fumarate impairs NF-κB signaling via reduced p65 nuclear translocalization and phosphorylation. Dimethyl fumarate inhibits maturation of DCs and subsequently Th1 and Th17 cell differentiation by suppression of both NF-κB and ERK1/2-MSK1 signaling[2].
    Dimethyl fumarate (DMF), an immune modulator and inducer of the antioxidant response, suppresses HIV replication and neurotoxin release[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Viability Assay[1]

    Cell Line: SGC-7901, HT29, HCT116 and CT26 cells
    Concentration: 20 μM, 50 μM, 100 μM, 200 μM
    Incubation Time: 24 hours
    Result: Reduced cell viability in SGC-7901, HT29, HCT116 and CT26 cancer cells.

    Western Blot Analysis[1]

    Cell Line: CT26 cancer cells
    Concentration: 100 μM
    Incubation Time: 3 hours, 6 hours, 12 hours, 24 hours
    Result: Significantly activated JNK, p38 and ERK in CT26 cells after treatment from 3 to 24 h.
    In Vivo

    Dimethyl fumarate (DMF; 50 mg/kg; oral gavage; daily; for 7 days) treatment is shown to upregulate the mRNA and protein levels of Nrf2 and Nrf2-regulated cytoprotective genes, attenuate 6-OHDA induced striatal oxidative stress and inflammation in C57BL/6 mice[4].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male C57BL/6 mice (8-week-old)[4]
    Dosage: 50 mg/kg
    Administration: Oral gavage; daily; for 7 days
    Result: Was shown to upregulate mRNA and protein levels of Nrf2 and Nrf2-regulated cytoprotective genes.
    Clinical Trial
    Molecular Weight

    144.13

    Formula

    C₆H₈O₄

    CAS No.

    624-49-7

    SMILES

    O=C(OC)/C=C/C(OC)=O

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 41.67 mg/mL (289.11 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 6.9382 mL 34.6909 mL 69.3818 mL
    5 mM 1.3876 mL 6.9382 mL 13.8764 mL
    10 mM 0.6938 mL 3.4691 mL 6.9382 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (17.35 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.08 mg/mL (14.43 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.08 mg/mL (14.43 mM); Clear solution

    • 4.

      Add each solvent one by one:  50% PEG300    50% saline

      Solubility: 7.5 mg/mL (52.04 mM); Suspended solution; Need ultrasonic

    *All of the co-solvents are provided by MCE.
    References

    Purity: ≥98.0%

    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.
    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass   Concentration   Volume   Molecular Weight *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
    C1   V1   C2   V2

    Keywords:

    DimethylDMFKeap1-Nrf2Reactive Oxygen SpeciesHIVAutophagyEndogenous MetaboliteHuman immunodeficiency virusAntioxidantneuroprotectiveanti-inflammatorysclerosisROSbrain-penetrantInhibitorinhibitorinhibit

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

     

    Salutation

    Applicant Name *

     

    Email address *

    Phone number *

     

    Organization name *

    Department *

     

    Requested quantity *

    Country or Region *

         

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    Dimethyl fumarate
    Cat. No.:
    HY-17363
    Quantity:
    MCE Japan Authorized Agent: