Flutamide (Synonyms: SCH 13521)

Name: Flutamide
Brand: MedChemExpress
SKU: HY-B0022
Rating: 5.0 (12 reviews)

Cat. No.: HY-B0022 Purity: 99.94%: Data Sheet
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Flutamide is an Androgen Receptor antagonist with Ki=55 nM. Flutamide inhibits prostate cancer progression and has synergistic effects with Docetaxel (HY-B0011). Flutamide also has the potential to protect against hyperthermia-induced multiple organ dysfunction syndrome.

For research use only. We do not sell to patients.

CAS No. : 13311-84-7

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	In-stock	Estimated Time of Arrival: December 31
25 g	In-stock	Estimated Time of Arrival: December 31
50 g	Get quote

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Based on 12 publication(s) in Google Scholar

Other Forms of Flutamide:

Flutamide (Standard) In-stock
Flutamide-d₇ Get quote

12 Publications Citing Use of MCE Flutamide

Histological Imaging/Staining

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RT-PCR

Cell Proliferation/Viability Assay

Bio/Physico-chemical Assay

: Flutamide purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2025 Aug 10:496:139488. [Abstract]; Flutamide (2000 μg/L; 28 d) caused widened interstitial spaces and vacuolation in zebrafish testis exposed to PFCAs.

: Flutamide purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2025 Aug 10:496:139488. [Abstract]; Flutamide (FT) (2000 μg/L; 28 d) significantly decreased serum T levels and increased E2 and VTG levels in zebrafish.

: Flutamide purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2025 Aug 10:496:139488. [Abstract]; Flutamide (FT) (2000 μg/L; 28 d) significantly downregulated androgen synthesis genes and AR pathway related genes (cyp17a1, star, hsd11b2, sox9a, ar and fkbp5) in zebrafish.

: Flutamide purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2025 Aug 10:496:139488. [Abstract]; Flutamide (FT) (1 nM-1 μM; 48 h) suppressed proliferation of LNCaP cells dose-dependently.

: Flutamide purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2025 Aug 10:496:139488. [Abstract]; Flutamide (FT) (1 nM-1 μM; 24 h) effectively suppressed the transcriptional activity of hAR enhanced by DHT in MDA-kb₂ cells.

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Description

Cellular Effect

Cell Line	Type	Value	Description	References
COS-7	IC₅₀	51.51 μM Compound: Flutamide	Anticancer activity against African green monkey COS7 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay Anticancer activity against African green monkey COS7 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay	[PMID: 32866675]
CV-1	IC₅₀	110 μM Compound: flutamide	Antagonist activity at human androgen receptor expressed in human CV1 cells assessed as inhibition of receptor-mediated transactivation by MMTV-luciferase reporter gene assay Antagonist activity at human androgen receptor expressed in human CV1 cells assessed as inhibition of receptor-mediated transactivation by MMTV-luciferase reporter gene assay	[PMID: 20826091]
HCT-15	IC₅₀	> 100 μM Compound: Flutamide	Anticancer activity against human HCT-15 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay Anticancer activity against human HCT-15 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay	[PMID: 32866675]
K562	IC₅₀	29.2 μM Compound: Flutamide	Anticancer activity against human K562 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay Anticancer activity against human K562 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay	[PMID: 32866675]
LNCaP	EC₅₀	7 μM Compound: Flutamide	Cytotoxicity against human LNCAP cells assessed as reduction in cell viability after 24 hrs by MTT assay Cytotoxicity against human LNCAP cells assessed as reduction in cell viability after 24 hrs by MTT assay	10.1039/C6MD00426A
LNCaP	EC₅₀	7 μM Compound: Flutamide	Cytotoxicity against human LNCAP cells after 24 hrs by MTT assay Cytotoxicity against human LNCAP cells after 24 hrs by MTT assay	[PMID: 28363155]
LNCaP	IC₅₀	0.9 μM Compound: Flutamide	Anticancer activity against human LNCAP cells after 24 hrs by MTT assay in presence of testosterone Anticancer activity against human LNCAP cells after 24 hrs by MTT assay in presence of testosterone	[PMID: 21600678]
LNCaP	IC₅₀	11600 nM Compound: Flutamide	Displacement of [3H]R-1881 from Androgen receptor of LNCaP cells Displacement of [3H]R-1881 from Androgen receptor of LNCaP cells	[PMID: 15828836]
LNCaP	IC₅₀	47.55 μM Compound: Flutamide	Cytotoxicity against human LNCAP cells after 48 hrs by MTT assay Cytotoxicity against human LNCAP cells after 48 hrs by MTT assay	[PMID: 28757062]
LNCaP	IC₅₀	81.8 μM Compound: Flutamide	Cytotoxicity against androgen-dependent human LNCAP cells after 72 hrs by SRB assay Cytotoxicity against androgen-dependent human LNCAP cells after 72 hrs by SRB assay	[PMID: 22672984]
MDA-MB-453	IC₅₀	2 μM Compound: Flutamide	Antiandrogenic activity in human MDA-MB-453 cells expressing androgen receptor assessed as inhibition of DHT-induced androgen-dependent transcription by luciferase reporter gene assay Antiandrogenic activity in human MDA-MB-453 cells expressing androgen receptor assessed as inhibition of DHT-induced androgen-dependent transcription by luciferase reporter gene assay	[PMID: 19131248]
NCI-H1299	IC₅₀	> 500 μM Compound: Flutamide	Anticancer activity against human H1299 cells assessed as inhibition of cell proliferation measured after 72 hrs by MTT assay Anticancer activity against human H1299 cells assessed as inhibition of cell proliferation measured after 72 hrs by MTT assay	[PMID: 34425478]
NIH3T3	IC₅₀	5.8 x 10^-7 M Compound: 1a, flu	Antagonist activity at human androgen receptor expressed in mouse NIH3T3 cells assessed as inhibition of DHT-induced transcriptional activation after 24 hrs by androgen response element-mediated luciferase reporter gene assay Antagonist activity at human androgen receptor expressed in mouse NIH3T3 cells assessed as inhibition of DHT-induced transcriptional activation after 24 hrs by androgen response element-mediated luciferase reporter gene assay	[PMID: 18707892]
PC-3	IC₅₀	20.3 μM Compound: Flutamide	Anticancer activity against human PC-3 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay Anticancer activity against human PC-3 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay	[PMID: 32866675]
PC-3	IC₅₀	58 μM Compound: Flutamide	Anticancer activity against human PC-3 cells assessed as inhibition of cell proliferation measured after 72 hrs by MTT assay Anticancer activity against human PC-3 cells assessed as inhibition of cell proliferation measured after 72 hrs by MTT assay	[PMID: 34425478]
PC-3	IC₅₀	67.34 μM Compound: Flutamide	Cytotoxicity against human PC3 cells after 48 hrs by MTT assay Cytotoxicity against human PC3 cells after 48 hrs by MTT assay	[PMID: 28757062]
PC-3	IC₅₀	98.8 μM Compound: Flutamide	Cytotoxicity against androgen-independent human PC3 cells after 72 hrs by SRB assay Cytotoxicity against androgen-independent human PC3 cells after 72 hrs by SRB assay	[PMID: 22672984]
SC-3	IC₅₀	4.2 μM Compound: 4, flutamide	Antagonist activity at wild type human recombinant androgen receptor assessed as inhibition of testosterone-induced growth of mouse androgen dependent SC3 cells by WST-1 method Antagonist activity at wild type human recombinant androgen receptor assessed as inhibition of testosterone-induced growth of mouse androgen dependent SC3 cells by WST-1 method	[PMID: 18571420]
SC-3	IC₅₀	7.2 x 10^-7 M Compound: 3	Growth inhibition of mouse SC3 cells after 3 days by WST-8 assay Growth inhibition of mouse SC3 cells after 3 days by WST-8 assay	[PMID: 20521823]
Sf21	IC₅₀	143.2 μM Compound: Flutamide	Inhibition of human BSEP expressed in plasma membrane vesicles of Sf21 cells assessed as inhibition of ATP-dependent [3H]taurocholate uptake Inhibition of human BSEP expressed in plasma membrane vesicles of Sf21 cells assessed as inhibition of ATP-dependent [3H]taurocholate uptake	[PMID: 21965623]
Sf21	IC₅₀	78.7 μM Compound: Flutamide	Inhibition of Sprague-Dawley rat Bsep expressed in plasma membrane vesicles of Sf21 cells assessed as inhibition of ATP-dependent [3H]taurocholate uptake Inhibition of Sprague-Dawley rat Bsep expressed in plasma membrane vesicles of Sf21 cells assessed as inhibition of ATP-dependent [3H]taurocholate uptake	[PMID: 21965623]
U-251	IC₅₀	22.11 μM Compound: Flutamide	Anticancer activity against human U-251 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay Anticancer activity against human U-251 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay	[PMID: 32866675]

In Vitro

The active metabolite of Flutamide, is Flutamide-OH. Both of them directly bind rat anterior pituitary androgen receptor (Ki=55 nM)^[1].
Flutamide does not affect the proliferation of an androgen-sensitive clone of the mouse mammary carcinoma Shionogi SC-l 15 cells in culture, shows only antiandrogenic effect, but not androgenic effect^[2].
Flutamide provides treatment for prostate cancer when used along with Leuprolide^[3].
Flutamide has cytotoxic activity against PC3 and LNCap (IC50s 20 μM and 12 μM, respectively). Flutamide (10 μM, 5 μM; 48 h) induces apoptosis and reduces cell migration and colonization in PC3 and LNCap cells^[4].
Flutamide also downregulates the expression of KLK2 and EMT pathway genes in cells^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Flutamide causes a markedly reduction in rat ventral prostate weight from 319 mg to 245 mg. A combination of Flutamide and LHRH agonist, induces an additive effect with a decrease in prostate weight to 101 mg, and an marked drop in prostatic ODC activity^[5].
Flutamide (12.5-50 mg/kg; sc; once daily for 3 days) alleviates heat stroke in heat-stressed mice^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

276.21

Formula

C₁₁H₁₁F₃N₂O₃

CAS No.

13311-84-7

Appearance

Solid

Color

Light yellow to yellow

SMILES

CC(C)C(NC1=CC=C([N+]([O-])=O)C(C(F)(F)F)=C1)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (362.04 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.6204 mL	18.1022 mL	36.2043 mL
5 mM	0.7241 mL	3.6204 mL	7.2409 mL
10 mM	0.3620 mL	1.8102 mL	3.6204 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.05 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.05 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (9.05 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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mg/kg

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μL

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.94%

Select Batch:

Data Sheet (276 KB) SDS (418 KB)

COA (247 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Simard J, et al. Characteristics of interaction of the antiandrogen flutamide with the androgen receptor in various target tissues. Mol Cell Endocrinol. 1986 Mar;44(3):261-70. [Content Brief]

[2]. Luthy IA, et al. Androgenic activity of synthetic progestins and spironolactone in androgen-sensitive mouse mammary carcinoma (Shionogi) cells in culture. J Steroid Biochem. 1988 Nov;31(5):845-52. [Content Brief]

[3]. Crawford ED, et al. A controlled trial of leuprolide with and without flutamide in prostatic carcinoma. N Engl J Med. 1989 Aug 17;321(7):419-24. [Content Brief]

[4]. Rahimnia R, et al. The effect of Ganoderma lucidum polysaccharide extract on sensitizing prostate cancer cells to flutamide and docetaxel: an in vitro study. Sci Rep. 2023 Nov 2;13(1):18940. [Content Brief]

[5]. Lin CY, et al. Flutamide, an androgen receptor antagonist, improves heatstroke outcomes in mice. Eur J Pharmacol. 2012 Aug 5;688(1-3):62-7. [Content Brief]

[6]. Marchetti B, et al. Characteristics of flutamide action on prostatic and testicular functions in the rat. J Steroid Biochem. 1988 Jun;29(6):691-8. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.6204 mL	18.1022 mL	36.2043 mL	90.5108 mL
	5 mM	0.7241 mL	3.6204 mL	7.2409 mL	18.1022 mL
	10 mM	0.3620 mL	1.8102 mL	3.6204 mL	9.0511 mL
	15 mM	0.2414 mL	1.2068 mL	2.4136 mL	6.0341 mL
	20 mM	0.1810 mL	0.9051 mL	1.8102 mL	4.5255 mL
	25 mM	0.1448 mL	0.7241 mL	1.4482 mL	3.6204 mL
	30 mM	0.1207 mL	0.6034 mL	1.2068 mL	3.0170 mL
	40 mM	0.0905 mL	0.4526 mL	0.9051 mL	2.2628 mL
	50 mM	0.0724 mL	0.3620 mL	0.7241 mL	1.8102 mL
	60 mM	0.0603 mL	0.3017 mL	0.6034 mL	1.5085 mL
	80 mM	0.0453 mL	0.2263 mL	0.4526 mL	1.1314 mL
	100 mM	0.0362 mL	0.1810 mL	0.3620 mL	0.9051 mL

Flutamide Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Flutamide13311-84-7SCH 13521SCH13521SCH-13521Androgen ReceptorInhibitorinhibitorinhibit

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Flutamide (Synonyms: SCH 13521)

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