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  3. Glycerol

Glycerol is used in sample preparation and gel formation for polyacrylamide gel electrophoresis.

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Glycerol

Glycerol Chemical Structure

CAS No. : 56-81-5

Size Prix Stock Quantité
Solvent
10 mM * 1 mL in DMSO En stock
Solvent
100 mL En stock
Solvent
500 mL En stock
1L   Obtenir un devis  
5L   Obtenir un devis  

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Based on 2 publication(s) in Google Scholar

Other Forms of Glycerol:

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Description

Glycerol is used in sample preparation and gel formation for polyacrylamide gel electrophoresis.

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

Glycerol is often included in polyacrylamide gels to prevent dissociation of nucleosomes and other protein-DNA complexes during electrophoresis. With glycerol included, fractionation seems to be largely based on particle mass and charge. The concentration of glycerol during electrophoresis strongly affects the separation characteristics of polyacrylamide gels[1]. Glycerol is an inevitable by-product of oils/fats processing, regardless of the pathway. Fermentative metabolism of glycerol has been studied in great detail in several species of the Enterobacteriaceae family, such as Citrobacter freundii and Klebsiella pneumoniae. The use of anaerobic fermentation to convert abundant and low-priced glycerol streams generated in the production of biodiesel into higher value products represents a promising route to achieve economic viability in the biofuels industry[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Glycerol can induce acute renal failure in rat models. Acute renal failure induced by glycerol or uranyl nitrate reduces the hepato-biliary transport of some drugs, modulates the distribution of drugs into the central nervous system and affects the activity of various hepatic microsomal enzymes [3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Masse moléculaire

92.09

Formule

C3H8O3

CAS No.
Appearance

Liquid (Density: 1.26 g/cm3)

Color

Colorless to light yellow

SMILES

OCC(O)CO

Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Store at room temperature 3 years

In solvent -80°C 2 years
-20°C 1 year
Solvant et solubilité
In Vitro: 

DMSO : 200 mg/mL (2171.79 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 10.8589 mL 54.2947 mL 108.5894 mL
5 mM 2.1718 mL 10.8589 mL 21.7179 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

  • Calculateur de molarité

  • Calculateur de dilution

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Pureté et documentation

Purity: 99.99%

Références
Administration animale
[3]

Rats: Experimental acute renal failure was induced in male Wistar rats 230-300 g by an injection of glycerol dissolved in saline (50% v/v, 10 mL/kg) into the leg muscle after a 24-h period of water deprivation[3].

MCE n'a pas confirmé de manière indépendante l'exactitude de ces méthodes. Ils sont pour référence seulement.

Références

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 10.8589 mL 54.2947 mL 108.5894 mL 271.4736 mL
5 mM 2.1718 mL 10.8589 mL 21.7179 mL 54.2947 mL
10 mM 1.0859 mL 5.4295 mL 10.8589 mL 27.1474 mL
15 mM 0.7239 mL 3.6196 mL 7.2393 mL 18.0982 mL
20 mM 0.5429 mL 2.7147 mL 5.4295 mL 13.5737 mL
25 mM 0.4344 mL 2.1718 mL 4.3436 mL 10.8589 mL
30 mM 0.3620 mL 1.8098 mL 3.6196 mL 9.0491 mL
40 mM 0.2715 mL 1.3574 mL 2.7147 mL 6.7868 mL
50 mM 0.2172 mL 1.0859 mL 2.1718 mL 5.4295 mL
60 mM 0.1810 mL 0.9049 mL 1.8098 mL 4.5246 mL
80 mM 0.1357 mL 0.6787 mL 1.3574 mL 3.3934 mL
100 mM 0.1086 mL 0.5429 mL 1.0859 mL 2.7147 mL
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Inquiry Information

Nom du produit:
Glycerol
Cat. No.:
HY-B1659
Quantité:
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