Guanidinosuccinic acid

Name: Guanidinosuccinic acid
Brand: MedChemExpress
SKU: HY-113373
Rating: 4.5 (1 reviews)

Cat. No.: HY-113373 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Guanidinosuccinic acid is a nitrogenous metabolite.

For research use only. We do not sell to patients.

Guanidinosuccinic acid Chemical Structure

CAS No. : 6133-30-8

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 55	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 32	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 120	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 180	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

Guanidinosuccinic acid is a nitrogenous metabolite.

IC₅₀ & Target

Human Endogenous Metabolite

In Vivo

Guanidinosuccinic acid, a constituent of normal urine, is elevated in the urine and serum of azotemic patients^[1]. Guanidinosuccinic acid (GSA), a guanidino compound found to be greatly increased in uremia, is administered by intraperitoneal (i.p.) injection to adult albino mice and to young mice 7, 14 and 21 days old. Epileptogenic and toxic properties are assessed and Guanidinosuccinic acid brain levels following i.p. injection ae determined. In adult mice, Guanidinosuccinic acid induces long-lasting generalized clonic and clonic-tonic convulsions in a dose-dependent manner with a CD₅₀ (and 95% confidence interval) of 363 (287-458) mg/kg (n=35), and an LD₅₀ of 579 (445-756) mg/kg. The CD₅₀ of Guanidinosuccinic acid corresponded with a brain concentration of 56 nmol/g tissue. Electrocorticographic recording in five adult mice revealed epileptiform discharges (spikes, spike-waves, and polyspike-waves) which appeared concomitant with the convulsions, When young mice are i.p. injected with a (for adults) subconvulsive dose of Guanidinosuccinic acid (250 mg/kg), an age-dependent decrease is noted in Guanidinosuccinic acid-induced convulsions and in the resulting brain concentration^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

175.14

Formula

C₅H₉N₃O₄

CAS No.

6133-30-8

Appearance

Solid

Color

White to off-white

SMILES

O=C(O)C[C@@H](C(O)=O)NC(N)=N

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 75 mg/mL (428.23 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 6 mg/mL (34.26 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.7097 mL	28.5486 mL	57.0972 mL
5 mM	1.1419 mL	5.7097 mL	11.4194 mL
10 mM	0.5710 mL	2.8549 mL	5.7097 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 9.09 mg/mL (51.90 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (273 KB) SDS (251 KB)

COA (246 KB) HNMR (244 KB)

Handling Instructions (2659 KB)

References

[1]. Milstien S, et al. Role of intestinal microflora in the metabolism of guanidinosuccinic acid. J Bacteriol. 1973 May;114(2):641-4. [Content Brief]

[2]. D'Hooge R, et al. Behavioral toxicity of guanidinosuccinic acid in adult and young mice. Toxicol Lett. 1992 Dec;64-65 Spec No:773-7. [Content Brief]

Animal Administration
^[2]

Mice^[2]
Randomly bred Swiss mice (male and female, body weight 13-25 g for the first series of experiments) are housed under standard environmentally controlled conditions. For another series of experiments, young Swiss mice 7, 14 and 21 days old are used. Injections of Guanidinosuccinic acid suspensions are delivered in volumes of 0.1 mL per 10 g body weight, i.p. and in doses between 250 and 1000 mg/kg (5 mice per dose, in duplicate). After injection, the mice are put in individual cylindrical transparent cages and observed for 1 h (for 2 h in the case of the young mice). Both CD₅₀ and LD₅₀ are calculated by probit analysis or moving average interpolation^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Milstien S, et al. Role of intestinal microflora in the metabolism of guanidinosuccinic acid. J Bacteriol. 1973 May;114(2):641-4. [Content Brief]

[2]. D'Hooge R, et al. Behavioral toxicity of guanidinosuccinic acid in adult and young mice. Toxicol Lett. 1992 Dec;64-65 Spec No:773-7. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	5.7097 mL	28.5486 mL	57.0972 mL	142.7429 mL
	5 mM	1.1419 mL	5.7097 mL	11.4194 mL	28.5486 mL
	10 mM	0.5710 mL	2.8549 mL	5.7097 mL	14.2743 mL
	15 mM	0.3806 mL	1.9032 mL	3.8065 mL	9.5162 mL
	20 mM	0.2855 mL	1.4274 mL	2.8549 mL	7.1371 mL
	25 mM	0.2284 mL	1.1419 mL	2.2839 mL	5.7097 mL
	30 mM	0.1903 mL	0.9516 mL	1.9032 mL	4.7581 mL
DMSO	40 mM	0.1427 mL	0.7137 mL	1.4274 mL	3.5686 mL
	50 mM	0.1142 mL	0.5710 mL	1.1419 mL	2.8549 mL
	60 mM	0.0952 mL	0.4758 mL	0.9516 mL	2.3790 mL
	80 mM	0.0714 mL	0.3569 mL	0.7137 mL	1.7843 mL
	100 mM	0.0571 mL	0.2855 mL	0.5710 mL	1.4274 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.