2. Apoptosis Autophagy
  3. Apoptosis Autophagy
  4. Hydroxysafflor yellow A

Hydroxysafflor yellow A  (Synonyms: Safflomin A; HSYA; ヒドロキシサフロールイエローA)

製品番号: HY-N0567 純度: 99.46%
COA 取扱説明書 Technical Support

Hydroxysafflor yellow A (Safflomin A) is a natural product of flavonoids isolated from safflower. Hydroxysafflor yellow A can inhibit cell proliferation and promote apoptosis through the autophagy pathway. Hydroxysafflor yellow A has anti-inflammatory, antioxidant and antitumor effects. Hydroxysafflor yellow A can be used in the study of cardiovascular disease.

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CAS 番号 : 78281-02-4

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 74 在庫あり
Solution
10 mM * 1 mL in DMSO USD 74 在庫あり
Solid
5 mg $55 在庫あり
10 mg $95 在庫あり
25 mg $185 在庫あり
50 mg $255 在庫あり
100 mg $390 在庫あり
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カスタマーレビュー

Based on 13 publication(s) in Google Scholar

Other Forms of Hydroxysafflor yellow A:

Hydroxysafflor yellow A 関連抗体

Top Publications Citing Use of Products

    Hydroxysafflor yellow A purchased from MedChemExpress. Usage Cited in: Drug Des Devel Ther. 2023 Jun 17:17:1819-1829.

    Cell viabilities in the Hydroxysafflor yellow A (HSYPA) (200 µM), Apigenin (20 µM), and Luteolin (10 µM) groups were all significantly lower in the absence of TGFβ1, while no differences were observed in the SYPA group.

    Hydroxysafflor yellow A purchased from MedChemExpress. Usage Cited in: Exp Ther Med. 2022 Nov 3;24(6):741.

    Representative photomicrographs of H&E stained kidney sections, pretreatment with Hydroxysafflor yellow A (HSYA) (100 mg/kg, i.p.) reduced the severity of the tubular injury.

    Hydroxysafflor yellow A purchased from MedChemExpress. Usage Cited in: Cell Biochem Biophys. 2020 Dec;78(4):511-520.  [Abstract]

    The invasive and migratory ability of KYSE-30 cells after being treated with Hydroxysafflor yellow A (HSYA) (20 μM, 24 h).

    Hydroxysafflor yellow A purchased from MedChemExpress. Usage Cited in: Cell Biochem Biophys. 2020 Dec;78(4):511-520.  [Abstract]

    Induction of apoptosis in KYSE-30 cells after Hydroxysafflor yellow A (HSYA) (20 μM, 24 h) treatment.

    Hydroxysafflor yellow A purchased from MedChemExpress. Usage Cited in: Cell Biochem Biophys. 2020 Dec;78(4):511-520.  [Abstract]

    After KYSE-30 cells were treated with 20 µM Hydroxysafflor yellow A (HSYA) for 24 h, the protein expression levels of ICAM1, MMP9, TNF-α, and VCAM1 were all upregulated when compared with that in NC group via western blot analysis.

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    製品説明

    Hydroxysafflor yellow A (Safflomin A) is a natural product of flavonoids isolated from safflower. Hydroxysafflor yellow A can inhibit cell proliferation and promote apoptosis through the autophagy pathway. Hydroxysafflor yellow A has anti-inflammatory, antioxidant and antitumor effects. Hydroxysafflor yellow A can be used in the study of cardiovascular disease[1][2][3][4][5][6].

    体外実験

    Hydroxysafflor yellow A (10 μM, 24 h) Inhibition of LPS-induced proliferation and migration of vascular smooth muscle cells by toll-like receptor-4 pathway[1].
    Hydroxysafflor yellow A (0.01-10 mg/L, 4-96 h) can promote the expression of fat-soluble specific enzyme HSL by increasing the activity of HSL promoter and inhibit the proliferation and adipogenesis of 3T3-L1 preadipocytes[2].
    Hydroxysafflor yellow A (1-100 μM), 12-24 h) decreases LPS-induced EC inflammatory damage by inhibiting p38 MAPK phosphorylation, NF-κB activation, high expression of inflammatory factors and leukocyte adhesion to EC[3].
    Hydroxysafflor yellow A (20-160 μM, 24-48 h) inhibits cell proliferation and promotes apoptosis by blocking the autophagy flux in liver cancer cells[6].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Hydroxysafflor yellow A 関連抗体

    Cell Migration Assay [1]

    Cell Line: VSMCs
    Concentration: 0.1, 1, 10,100 μM
    Incubation Time: 24 h
    Result: Inhibited LPS-induced VSMCs proliferation and migration with the concentration of 10 μM.

    Western Blot Analysis[1]

    Cell Line: VSMCs
    Concentration: 10 μM
    Incubation Time: 24 h
    Result: Inhibited LPS-induced upregulation of pro-inflammatory factors (TNF-α, IL-6, and IL-8).
    Inhibited LPS-induced upregulation of TLR-4 and activation of Rac1/Akt pathway.

    Cell Viability Assay[2]

    Cell Line: 3T3-L1
    Concentration: 0.01, 0.1, 1, 10 mg/L
    Incubation Time: 4, 8, 24, 48, 72, 96 h
    Result: Inhibit the cell viability in a dose and time-dependent manner.

    Apoptosis Analysis[6]

    Cell Line: HepG2, Huh7
    Concentration: 20, 40, 80, 160 μM
    Incubation Time: 24 h
    Result: Suppressed the growth and induced apoptotic cell death in both concentration and time-dependent manners.
    Augmented the induction of autophagosomes.
    体内実験

    Hydroxysafflor yellow A (2.5 or 10 mg/kg/day, intraperitoneal injection for 6 weeks) protects the liver from long-term alcohol damage by enhancing antioxidant capacity and inhibiting TGF-β1 expression[4].
    Hydroxysafflor yellow A (30, 48, 76.8 mg/kg, intraperitoneal injection) has a protective effect on inflammatory damage in rats with chronic obstructive pulmonary disease by reducing NF-κB and p38MAPK signal transduction[5].
    Hydroxysafflor yellow A (1.135, 2.25 mg/kg, intraperitoneal injection) can inhibit tumor growth and block autophagy of liver cancer cells in tumor models of nude mice[6].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Liver injury model induced by alcohol[4]
    Dosage: 2.5 or 10 mg/kg
    Administration: i.p. daily for 6 weeks
    Result: Decreased the level of ALT and AST.
    Decreased the levels of HA, LN, and III-C.
    Increased the activities of SOD and GPx and decreased the level of TGF-β1 expression.
    Animal Model: Chronic obstructive pulmonary disease (COPD)[5]
    Dosage: 30, 48, 76.8 mg/kg
    Administration: i.p.
    Result: Inhibited the elevated IL-6, IL-1β, TNF-α, ICAM-1 and VCAM-1 mRNA Levels.
    Inhibited the increased phosphorylation and NF-κB p65 levels.
    分子量

    612.53

    分子式

    C27H32O16
    CAS 番号
    Appearance

    Solid

    Color

    Yellow to orange

    SMILES

    OC(C(O)=C1C(/C=C/C2=CC=C(O)C=C2)=O)([C@]([C@@H]([C@@H](O)[C@@H]3O)O)([H])O[C@@H]3CO)C(O)=C([C@@H]([C@@H]([C@@H](O)[C@@H]4O)O)O[C@@H]4CO)C1=O
    Structure Classification
    Initial Source
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
    溶剤 & 溶解度
    体外: 

    H2O : ≥ 100 mg/mL (163.26 mM)

    DMSO : 87.5 mg/mL (142.85 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.6326 mL 8.1629 mL 16.3257 mL
    5 mM 0.3265 mL 1.6326 mL 3.2651 mL
    10 mM 0.1633 mL 0.8163 mL 1.6326 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

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    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (3.40 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (3.40 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    (per animal)

    g

    Dosing volume
    (per animal)

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    純度とドキュメンテーション

    純度: 99.46%

    COA (256 KB) HNMR (300 KB) RP-HPLC (247 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 1.6326 mL 8.1629 mL 16.3257 mL 40.8143 mL
    5 mM 0.3265 mL 1.6326 mL 3.2651 mL 8.1629 mL
    10 mM 0.1633 mL 0.8163 mL 1.6326 mL 4.0814 mL
    15 mM 0.1088 mL 0.5442 mL 1.0884 mL 2.7210 mL
    20 mM 0.0816 mL 0.4081 mL 0.8163 mL 2.0407 mL
    25 mM 0.0653 mL 0.3265 mL 0.6530 mL 1.6326 mL
    30 mM 0.0544 mL 0.2721 mL 0.5442 mL 1.3605 mL
    40 mM 0.0408 mL 0.2041 mL 0.4081 mL 1.0204 mL
    50 mM 0.0327 mL 0.1633 mL 0.3265 mL 0.8163 mL
    60 mM 0.0272 mL 0.1360 mL 0.2721 mL 0.6802 mL
    80 mM 0.0204 mL 0.1020 mL 0.2041 mL 0.5102 mL
    100 mM 0.0163 mL 0.0816 mL 0.1633 mL 0.4081 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Hydroxysafflor yellow A Related Classifications

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    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
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      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Hydroxysafflor yellow A78281-02-4Safflomin A HSYAApoptosisAutophagyHSYAProliferation3T3-L1VSMCsECHepG2Huh7Chronic obstructive pulmonary diseaseInhibitorinhibitorinhibit

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    製品名:
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    製品番号:
    HY-N0567
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