Cat. No.: HY-B1490

Imipramine hydrochloride

6 Cited Publications

Customer Review

Based on 6 publication(s) in Google Scholar

Imipramine hydrochloride is an orally active tertiary amine tricyclic antidepressant. Imipramine hydrochloride is a Fascin1 inhibitor with antitumor activities. Imipramine hydrochloride also inhibits serotonin transporter with an IC₅₀ value of 32 nM. Imipramine hydrochloride stimulates U-87MG glioma cells autophagy and induces HL-60 cell apoptosis. Imipramine hydrochloride shows neuroprotective and immunomodulatory effects.

For research use only. We do not sell to patients.

Purity: 99.82%
CAS No.: 113-52-0
Formula: C₁₉H₂₅ClN₂
Molecular Weight:316.87

Storage:

4°C, sealed storage, away from moisture

* In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture)

Imipramine hydrochloride Chemical Structure

CAS No.: 113-52-0

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (6)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

Fascin1, Serotonin Autophagy, Apoptosis^[1]^[2]^[3]^[5]
IC₅₀: 32 nM (human placental serotonin transporter)^[5]

In Vitro

Imipramine (0.5-300 μM, 3 days) inhibits HCT-116 cell viability^[1].
Imipramine (20 μM) inhibits cell migration (7 h) and invasion (48 h)^[1].
Imipramine (50 μM, 0-240 min) inhibites the PI3K/Akt/mTOR signaling pathway in U-87MG glioma cells^[2].
Imipramine (60 μM, 24 h) stimulates U-87MG glioma cells autophagy^[2].
Imipramine (80 μM, 24 h) induces HL-60 cell apoptosis^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

DLD-1, HCT-116, and SW-480
Concentration:

0.5-300 μM
Incubation Time:

3 days
Result:

Inhibited cell viability and HCT-116 was more sensitive than DLD-1 and SW-480.

Cell Migration Assay ^[1]

Cell Line:

SW-480, DLD-1, and HCT-116
Concentration:

20 μM
Incubation Time:

7 h
Result:

Produced a remarkable inhibition of migration in all assayed cell lines.

Cell Invasion Assay^[1]

Cell Line:

HCT-116
Concentration:

20 μM
Incubation Time:

48 h
Result:

Inhibited cell invasion through Matrigel.

Western Blot Analysis^[2]

Cell Line:

U-87MG
Concentration:

50 μM
Incubation Time:

0, 15, 30, 60, 120 and 240 min
Result:

Markedly inhibited the phosphorylation of both Akt (Ser473) and mTOR (Ser2481) in a time-dependent manner. Also dephosphorylated p70 S6K, a downstream target of mTOR.

Cell Autophagy Assay^[2]

Cell Line:

U-87MG
Concentration:

60 μM
Incubation Time:

24 h
Result:

Stimulated the induction of autophagy through the redistribution of LC3 in U-87MG glioma cells.

Cell Autophagy Assay^[3]

Cell Line:

HL-60
Concentration:

80 μM
Incubation Time:

24 h
Result:

Induced cell apoptosis.

In Vivo

Imipramine (20 mg/kg, i.p. or 15 mg/kg, p.o.; daily for 24 days) attenuates neuroinflammatory signaling and reverses stress-induced social avoidance in mice^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male C57BL/6 mice (6–8 weeks old) subjected to RSD (repeated social defeat) and HCC (home cage control)^[4]
Dosage:

20 mg/kg or 15 mg/kg
Administration:

Intraperitoneal injection or oral administration, daily for 24 days
Result:

Reversed RSD-induced social avoidance behavior, significantly increasing the interaction time, significantly decreased stress-induced mRNA levels for IL-6 in brain microglia.

Chemical Information

CAS No. 113-52-0
Appearance Solid
Molecular Weight 316.87
Formula C₁₉H₂₅ClN₂
Color White to off-white
SMILES

CN(C)CCCN1C2=CC=CC=C2CCC3=CC=CC=C31.[H]Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

* In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture)

Publications (6)

All WBCell Proliferation/Viability AssayCell Imaging/StainingCell Migration/Invasion AssayFlow CytometryIn Vivo Efficacy StudyIHC

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Journal Impact Factor

Nat Chem Biol

Pharmacological inhibition of Kir4.1 evokes rapid-onset antidepressant responses. [Abstract]

2024 Jul;20(7):857-866. PMID: 38355723
Cell Commun Signal

Tricyclic antidepressants induce liver inflammation by targeting NLRP3 inflammasome activation. [Abstract]

2023 May 25;21(1):123. PMID: 37231437

Imipramine hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2023 May 25;21(1):123. [Abstract]

LPS-primed BMDMs were stimulated with Imipramine (40 μM, 12 h). Western blot assessed the expression of caspase-1 and IL-1β in SN and GSDMD cleavage in WCL.

Imipramine hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2023 May 25;21(1):123. [Abstract]

BMDMs were incubated with LPS and MCC950 and then treated with Imipramine (40 μM, 12 h). The expressions of IL-1β and caspase-1 in SN as well as GSDMD cleavage in WCL were evaluated using western blotting.
J Agric Food Chem

β-Sitosterol Inhibits Tumor Growth and Amplifies Rituximab Sensitivity through Acid Sphingomyelinase/Ceramide Signaling in Diffuse Large B-Cell Lymphoma. [Abstract]

2024 Jul 24;72(29):16177-16190. PMID: 38991150
Inflammation

Acute Diallyl Disulfide Administration Prevents and Reveres Lipopolysaccharide-Induced Depression-Like Behaviors in Mice via Regulating Neuroinflammation and Oxido-Nitrosative Stress. [Abstract]

2021 Aug;44(4):1381-1395. PMID: 33511484
Int Immunopharmacol

Innovative role of the antidepressant imipramine in esophageal squamous cell carcinoma treatment: Promoting apoptosis and protective autophagy. [Abstract]

2025 Jan 6:147:113969. PMID: 39764996

Imipramine hydrochloride purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2025 Jan 6:147:113969. [Abstract]

Cells were exposed to moclobemide, milnacipran, venlafaxine, escitalopram, amitriptyline, trazodone, fluvoxamine, or Imipramine (0–100 μM concentration) for 24 h. Cell viability was assessed with CCK-8 assays.

Imipramine hydrochloride purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2025 Jan 6:147:113969. [Abstract]

CCK-8 assays for 24 h, 48 h and 72 h in KYSE30 and KYSE150 cells treated with various concentrations of Imipramine.

Imipramine hydrochloride purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2025 Jan 6:147:113969. [Abstract]

Colony formation assays in KYSE30 and KYSE150 cells treated with Imipramine (0, 50, or 100 μM, 14 days) . Histograms represent the number of colonies per set of replicates, according to colony formation measurements (n = 3).

Imipramine hydrochloride purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2025 Jan 6:147:113969. [Abstract]

Imipramine (0, 50, or 100 μM, 48 h). KYSE30 and KYSE150 cells showing migration or invasion in Transwell assays were fixed and stained. Cells that crossed the membranes were counted under a light microscope (10 × ) in three random fields of view.

Imipramine hydrochloride purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2025 Jan 6:147:113969. [Abstract]

Imipramine (0, 50, or 100 μM, 48 h) induces apoptosis in ESCC cells. ESCC or HET-1A cells were treated with various concentrations of Imipramine for 48 h. Cell apoptosis was detected by flow cytometry with annexin V-FITC/Caspase-3 double staining.

Imipramine hydrochloride purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2025 Jan 6:147:113969. [Abstract]

ESCC cells were treated with various concentrations of Imipramine (50, 100 μM) for 48 h. Western blotting was performed to assess Bax, Bcl-2, and cleaved-caspase-3 expression.

Imipramine hydrochloride purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2025 Jan 6:147:113969. [Abstract]

Anti-tumor effect of Imipramine (receiving intraperitoneal injections of 20 mg/kg/day, 15 days) in a xenograft model.Representative images depicting the appearance of tumors at the conclusion of treatment. Tumor volumes were measured every 3 days. Final tumor weights.

Imipramine hydrochloride purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2025 Jan 6:147:113969. [Abstract]

Imipramine (receiving intraperitoneal injections of 20 mg/kg/day, 15 days). Expression of P62, ATG5-ATG12 complex, Bcl-2, Bax, and Ki-67 was detected by IHC staining in tumor tissues.
Pathogens

Redirecting Imipramine against Bluetongue Virus Infection: Insights from a Genome-wide Haploid Screening Study. [Abstract]

2022 May 22;11(5):602. PMID: 35631123

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (315.59 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 62.5 mg/mL (197.24 mM; Need ultrasonic)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.1559 mL

15.7793 mL

31.5587 mL

5 mM

0.6312 mL

3.1559 mL

6.3117 mL

10 mM

0.3156 mL

1.5779 mL

3.1559 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (7.89 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (7.89 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (7.89 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 60 mg/mL (189.35 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (289 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Alburquerque-González B, et al. New role of the antidepressant imipramine as a Fascin1 inhibitor in colorectal cancer cells. Exp Mol Med. 2020 Feb;52(2):281-292. [Content Brief]

[2]. Jeon SH, et al. The tricyclic antidepressant imipramine induces autophagic cell death in U-87MG glioma cells. Biochem Biophys Res Commun. 2011 Sep 23;413(2):311-7. [Content Brief]

[3]. Xia Z, et al. The antidepressants imipramine, clomipramine, and citalopram induce apoptosis in human acute myeloid leukemia HL-60 cells via caspase-3 activation. J Biochem Mol Toxicol. 1999;13(6):338-47. [Content Brief]

[4]. Ramirez K, et al. Imipramine attenuates neuroinflammatory signaling and reverses stress-induced social avoidance. Brain Behav Immun. 2015 May;46:212-20. [Content Brief]

[5]. Balkovetz DF, et al. Evidence for an imipramine-sensitive serotonin transporter in human placental brush-border membranes. J Biol Chem. 1989 Feb 5;264(4):2195-8. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	3.1559 mL	15.7793 mL	31.5587 mL	78.8967 mL
	5 mM	0.6312 mL	3.1559 mL	6.3117 mL	15.7793 mL
	10 mM	0.3156 mL	1.5779 mL	3.1559 mL	7.8897 mL
	15 mM	0.2104 mL	1.0520 mL	2.1039 mL	5.2598 mL
	20 mM	0.1578 mL	0.7890 mL	1.5779 mL	3.9448 mL
	25 mM	0.1262 mL	0.6312 mL	1.2623 mL	3.1559 mL
	30 mM	0.1052 mL	0.5260 mL	1.0520 mL	2.6299 mL
	40 mM	0.0789 mL	0.3945 mL	0.7890 mL	1.9724 mL
	50 mM	0.0631 mL	0.3156 mL	0.6312 mL	1.5779 mL
	60 mM	0.0526 mL	0.2630 mL	0.5260 mL	1.3149 mL
	80 mM	0.0394 mL	0.1972 mL	0.3945 mL	0.9862 mL
	100 mM	0.0316 mL	0.1578 mL	0.3156 mL	0.7890 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

Imipramine hydrochloride

6 Cited Publications

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Imipramine hydrochloride

Apoptosis Compound Library

Neuronal Signaling Compound Library

FDA-Approved Drug Library

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Publications Citing Use of MedChemExpress (MCE) Imipramine hydrochloride

Biological Activity

Chemical Information

Publications (6)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Imipramine hydrochloride Related Classifications

HY-B1490 Related Classifications