2. Imipramine

Imipramine is an orally active tertiary amine tricyclic antidepressant. Imipramine is a Fascin1 inhibitor with antitumor activities. Imipramine also inhibits serotonin transporter with an IC50 value of 32 nM. Imipramine stimulates U-87MG glioma cells autophagy and induces HL-60 cell apoptosis. Imipramine shows neuroprotective and immunomodulatory effects.

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Imipramine Chemical Structure

Imipramine Chemical Structure

CAS No. : 50-49-7

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Based on 4 publication(s) in Google Scholar

Other Forms of Imipramine:

Imipramine Related Antibodies

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Description

Imipramine is an orally active tertiary amine tricyclic antidepressant. Imipramine is a Fascin1 inhibitor with antitumor activities. Imipramine also inhibits serotonin transporter with an IC50 value of 32 nM. Imipramine stimulates U-87MG glioma cells autophagy and induces HL-60 cell apoptosis. Imipramine shows neuroprotective and immunomodulatory effects[1][2][3][4][5].

IC50 & Target

Fascin1, Serotonin Transporter, Autophagy, Apoptosis[1][2][3][5]
IC50: 32 nM (human placental serotonin transporter)[5]
In Vitro

Imipramine (0.5-300 μM, 3 days) inhibits HCT-116 cell viability[1].
Imipramine (20 μM) inhibits cell migration (7 h) and invasion (48 h)[1].
Imipramine (50 μM, 0-240 min) inhibites the PI3K/Akt/mTOR signaling pathway in U-87MG glioma cells[2].
Imipramine (60 μM, 24 h) stimulates U-87MG glioma cells autophagy[2].
Imipramine (80 μM, 24 h) induces HL-60 cell apoptosis[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Imipramine Related Antibodies

Cell Viability Assay[1]

Cell Line: DLD-1, HCT-116, and SW-480
Concentration: 0.5-300 μM
Incubation Time: 3 days
Result: Inhibited cell viability and HCT-116 was more sensitive than DLD-1 and SW-480.

Cell Migration Assay [1]

Cell Line: DLD-1, HCT-116, and SW-480
Concentration: 20 μM
Incubation Time: 7 h
Result: Produced a remarkable inhibition of migration in all assayed cell lines.

Cell Invasion Assay[1]

Cell Line: HCT-116
Concentration: 20 μM
Incubation Time: 48 h
Result: Inhibited cell invasion through Matrigel.

Western Blot Analysis[2]

Cell Line: U-87MG
Concentration: 50 μM
Incubation Time: 0, 15, 30, 60, 120 and 240 min
Result: Markedly inhibited the phosphorylation of both Akt (Ser473) and mTOR (Ser2481) in a time-dependent manner. Also dephosphorylated p70 S6K, a downstream target of mTOR.

Cell Autophagy Assay[2]

Cell Line: U-87MG
Concentration: 60 μM
Incubation Time: 24 h
Result: Stimulated the induction of autophagy through the redistribution of LC3 in U-87MG glioma cells.

Apoptosis Analysis[3]

Cell Line: HL-60
Concentration: 80 μM
Incubation Time: 24 h
Result: Induced cell apoptosis.
In Vivo

Imipramine (20 mg/kg, i.p. or 15 mg/kg, p.o.; daily for 24 days) attenuates neuroinflammatory signaling and reverses stress-induced social avoidance in mice[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male C57BL/6 mice (6–8 weeks old) subjected to RSD (repeated social defeat) and HCC (home cage control)[4]
Dosage: 20 mg/kg or 15 mg/kg
Administration: Intraperitoneal injection or oral administration, daily for 24 days
Result: Reversed RSD-induced social avoidance behavior, significantly increasing the interaction time, significantly decreased stress-induced mRNA levels for IL-6 in brain microglia.
Clinical Trial
Molecular Weight

280.41

Formula

C19H24N2
CAS No.
Appearance

Liquid

Color

Colorless to light yellow

SMILES

CN(C)CCCN1C2=CC=CC=C2CCC3=CC=CC=C31
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Pure form -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (891.55 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.5662 mL 17.8310 mL 35.6621 mL
5 mM 0.7132 mL 3.5662 mL 7.1324 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.5662 mL 17.8310 mL 35.6621 mL 89.1552 mL
5 mM 0.7132 mL 3.5662 mL 7.1324 mL 17.8310 mL
10 mM 0.3566 mL 1.7831 mL 3.5662 mL 8.9155 mL
15 mM 0.2377 mL 1.1887 mL 2.3775 mL 5.9437 mL
20 mM 0.1783 mL 0.8916 mL 1.7831 mL 4.4578 mL
25 mM 0.1426 mL 0.7132 mL 1.4265 mL 3.5662 mL
30 mM 0.1189 mL 0.5944 mL 1.1887 mL 2.9718 mL
40 mM 0.0892 mL 0.4458 mL 0.8916 mL 2.2289 mL
50 mM 0.0713 mL 0.3566 mL 0.7132 mL 1.7831 mL
60 mM 0.0594 mL 0.2972 mL 0.5944 mL 1.4859 mL
80 mM 0.0446 mL 0.2229 mL 0.4458 mL 1.1144 mL
100 mM 0.0357 mL 0.1783 mL 0.3566 mL 0.8916 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Imipramine50-49-7Inhibitorinhibitorinhibit

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