Kuromanin chloride (Synonyms: Chrysontemin; Cyanidin 3-O-glucoside chloride)

Name: Kuromanin chloride
Brand: MedChemExpress
SKU: HY-N0640
Rating: 5.0 (10 reviews)

Art. -Nr.: HY-N0640 Reinheit: 99.83%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Kuromanin chloride (Chrysontemin) is derived from mulberry leaves and has the effect of increasing blood sugar concentration and maintaining lipid metabolism balance to reduce obesity. Kuromanin chloride can inhibit CD38 enzymatic activities.

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CAS. Nr. : 7084-24-4

Größe	Verfügbarkeit	Menge

Kostenlose Probe (0.1 - 0.2 mg)	Jetzt bewerben
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	Auf Lager	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	Auf Lager	Estimated Time of Arrival: December 31
Solid
2 mg	Auf Lager	Estimated Time of Arrival: December 31
5 mg	Auf Lager	Estimated Time of Arrival: December 31
10 mg	Auf Lager	Estimated Time of Arrival: December 31
25 mg	Auf Lager	Estimated Time of Arrival: December 31
50 mg	Auf Lager	Estimated Time of Arrival: December 31
100 mg	Auf Lager	Estimated Time of Arrival: December 31
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Based on 10 publication(s) in Google Scholar

Other Forms of Kuromanin chloride:

Kuromanin chloride (Standard) Angebot einholen

10 Publications Citing Use of MCE Kuromanin chloride

Cell Proliferation/Viability Assay

ELISA

Apoptosis Analysis

: Kuromanin chloride purchased from MedChemExpress. Usage Cited in: Can J Physiol Pharmacol. 2021 Jun;99(6):589-598. [Abstract]; MPC5 cells were cultured in 96-well plates and treated with indicated concentration of glucose (normal glucose: NG, 5.6 mM; high glucose: HG, 25 mM) in the presence or absence of different concentrations of Kuromanin chloride (C3G) (25, 50, 100 μM) for 24 h. The survival rate of different groups was tested with an MTT assay.

: Kuromanin chloride purchased from MedChemExpress. Usage Cited in: Can J Physiol Pharmacol. 2021 Jun;99(6):589-598. [Abstract]; MPC5 cells were cultured in 24-well plates and treated with indicated concentrations of glucose (NG:5.6 mM; HG: 25 mM) in the presence or absence of 50 μM concentration of Kuromanin chloride (C3G) for 24 h. The cell apoptosis rate of different groups was tested by flow cytometry.

: Kuromanin chloride purchased from MedChemExpress. Usage Cited in: Can J Physiol Pharmacol. 2021 Jun;99(6):589-598. [Abstract]; Kuromanin chloride (C3G) promotes autophagy in mouse podocytes under high glucose condition. MPC5 cells were treated with normal glucose (NG, 5.6 mM) or high glucose (HG, 25 mM) in the presence or absence of 50 μM C3G for 24 h. Measurement of LC3 foci level by immunofluorescence staining assay in MPC5 cells.

: Kuromanin chloride purchased from MedChemExpress. Usage Cited in: Drug Des Devel Ther. 2020 Aug 19;14:3385-3391. [Abstract]; Kuromanin chloride (C3G) (100 mg/kg, i.g.) administration suppressed CSE expression and deactivated the AMPK/mTOR pathway.

: Kuromanin chloride purchased from MedChemExpress. Usage Cited in: Drug Des Devel Ther. 2020 Aug 19;14:3385-3391. [Abstract]; Kuromanin chloride (C3G) (100 mg/kg, i.g.) induced cytokine production TNF-α and IL-6 by ELISA.

Spezialitätsempfehlungen

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biologische Aktivität
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Beschreibung

Cellular Effect

Cell Line	Type	Value	Description	References
HCT-116	IC₅₀	100 μM Compound: 3	Cytotoxicity against human HCT 116 cells after 72 hrs Cytotoxicity against human HCT 116 cells after 72 hrs	[PMID: 16933884]
HT-29	IC₅₀	100 μM Compound: 3	Cytotoxicity against human HT-29 cells after 72 hrs Cytotoxicity against human HT-29 cells after 72 hrs	[PMID: 16933884]
SW480	IC₅₀	100 μM Compound: 3	Cytotoxicity against human SW480 cells after 72 hrs Cytotoxicity against human SW480 cells after 72 hrs	[PMID: 16933884]

In Vitro

Kuromanin chloride (5 μg/mL) reduces the expression of Ad36 E1A viral gene in 3T3L1 cells infected with 4 MOI of Ad36^[1].
Kuromanin chloride (100 μM, 24 h) protects cerebellar granule neurons from nitric oxide (NO)-induced apoptosis^[2].
Kuromanin chloride (30 and 100 μM, 30 min) inhibits enzymatic activities of CD38, and blocks chronic lymphocytic leukemia (CLL) chemotaxis in CD38+ CLL cells (a fluorimetric assay based on cGDPR generation from NGD)^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molekulargewicht

484.84

Formel

C₂₁H₂₁ClO₁₁

CAS. Nr.

7084-24-4

Appearance

Solid

Color

Brown to dark brown

SMILES

O[C@H]([C@@H](O)[C@@H]1O)[C@@H](O[C@@H]1CO)OC(C(C2=CC(O)=C(O)C=C2)=[O+]C3=CC(O)=C4)=CC3=C4O.[Cl-]

Structure Classification

Initial Source

Microorganisms

Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 25 mg/mL (51.56 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0625 mL	10.3127 mL	20.6254 mL
5 mM	0.4125 mL	2.0625 mL	4.1251 mL
10 mM	0.2063 mL	1.0313 mL	2.0625 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner
Verdünnungsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.29 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.29 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Reinheit & Dokumentation

Purity: 99.83%

Select Batch:

Data Sheet (280 KB) SDS (393 KB)

COA (257 KB) HNMR (270 KB) RP-HPLC (257 KB) MS (69 KB)

Handling Instructions (2659 KB)

Verweise

[1]. Ha -Na Na, et al. Reduction of adenovirus 36-induced obesity andin?ammation by mulberry extract. Microbiol Immunol 2014; 58: 303–306

[2]. Winter AN, et al. Chemical basis for the disparate neuroprotective effects of the anthocyanins, callistephin and kuromanin, against nitrosative stress. Free Radic Biol Med. 2017 Feb;103:23-34. [Content Brief]

[3]. Vaisitti T, et al. The enzymatic activities of CD38 enhance CLL growth and trafficking: implications for therapeutic targeting. Leukemia. 2015 Feb;29(2):356-68. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.0625 mL	10.3127 mL	20.6254 mL	51.5634 mL
	5 mM	0.4125 mL	2.0625 mL	4.1251 mL	10.3127 mL
	10 mM	0.2063 mL	1.0313 mL	2.0625 mL	5.1563 mL
	15 mM	0.1375 mL	0.6875 mL	1.3750 mL	3.4376 mL
	20 mM	0.1031 mL	0.5156 mL	1.0313 mL	2.5782 mL
	25 mM	0.0825 mL	0.4125 mL	0.8250 mL	2.0625 mL
	30 mM	0.0688 mL	0.3438 mL	0.6875 mL	1.7188 mL
	40 mM	0.0516 mL	0.2578 mL	0.5156 mL	1.2891 mL
	50 mM	0.0413 mL	0.2063 mL	0.4125 mL	1.0313 mL

Kuromanin chloride Related Classifications

Neurological Disease Metabolic Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Kuromanin7084-24-4ChrysonteminCyanidin 3-O-glucosideCD38neurodegenerativeInhibitorinhibitorinhibit

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Kuromanin chloride (Synonyms: Chrysontemin; Cyanidin 3-O-glucoside chloride)

Kundenbewertung

Other Forms of Kuromanin chloride:

Kuromanin chloride Related Antibodies

10 Publications Citing Use of MCE Kuromanin chloride

Spezialitätsempfehlungen

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biologische Aktivität

Reinheit & Dokumentation

Verweise

Kundenbewertung

Kuromanin chloride Related Antibodies

Molaritätsrechner

Verdünnungsrechner

Complete Stock Solution Preparation Table

Kuromanin chloride Related Classifications

Keywords:

Ihre kürzlich angesehenen Produkte:

Kundenbewertung

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SAFETY DATA SHEET (SDS)

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