1. Anti-infection Metabolic Enzyme/Protease
  2. Parasite Lactate Dehydrogenase
  3. Nifurtimox

ニフルチモックス  (Synonyms: Nifurtimox)

製品番号: HY-W040073 純度: 99.65%
COA 取扱説明書

Nifurtimox, an antiprotozoal agent, which is generally used for the treatment of infections with Trypanosoma cruzi, has been used in the therapy of neuroblastoma. Nifurtimox affects enzyme activity of lactate dehydrogenase (LDH).

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

Nifurtimox 構造式

ニフルチモックス 構造式

CAS 番号 : 23256-30-6

容量 価格(税別) 在庫状況 数量
無料サンプル (0.1 - 0.5 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
USD 110 在庫あり
Solution
10 mM * 1 mL in DMSO USD 110 在庫あり
Solid
5 mg USD 100 在庫あり
10 mg USD 160 在庫あり
25 mg USD 350 在庫あり
50 mg USD 550 在庫あり
100 mg USD 850 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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カスタマーレビュー

Based on 1 publication(s) in Google Scholar

Other Forms of Nifurtimox:

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製品説明

Nifurtimox, an antiprotozoal agent, which is generally used for the treatment of infections with Trypanosoma cruzi, has been used in the therapy of neuroblastoma. Nifurtimox affects enzyme activity of lactate dehydrogenase (LDH).

IC50 & Target

Trypanosoma

 

Cellular Effect
Cell Line Type Value Description References
CHO GI50
13.9 μg/mL
Compound: nifurtimox
Cytotoxicity against CHO cells assessed as cell viability after 48 hrs by MTT colorimetric assay
Cytotoxicity against CHO cells assessed as cell viability after 48 hrs by MTT colorimetric assay
[PMID: 22551062]
Fibroblast IC50
> 200 μM
Compound: NFX
Cytotoxicity against human fibroblasts assessed as growth inhibition after 24 hrs by MTT assay
Cytotoxicity against human fibroblasts assessed as growth inhibition after 24 hrs by MTT assay
[PMID: 23644203]
H9c2 IC50
> 50 μM
Compound: Nifurtimox
Cytotoxicity against rat H9c2 cells infected with Trypanosoma cruzi Tulahuen amastigote forms assessed as reduction in cell number after 72 hrs by DRAQ5 DNA dye based confocal microscopic analysis
Cytotoxicity against rat H9c2 cells infected with Trypanosoma cruzi Tulahuen amastigote forms assessed as reduction in cell number after 72 hrs by DRAQ5 DNA dye based confocal microscopic analysis
[PMID: 30100019]
HEK293 EC50
> 100 μM
Compound: Nifurtimox
Cytotoxicity against HEK293 cells assessed as reduction in cell viability after 48 hrs by alamar blue assay
Cytotoxicity against HEK293 cells assessed as reduction in cell viability after 48 hrs by alamar blue assay
[PMID: 27720295]
HeLa IC50
12 μM
Compound: Nfx
Cytotoxicity against human HeLa cells after 24 hrs by MTT assay
Cytotoxicity against human HeLa cells after 24 hrs by MTT assay
[PMID: 19168363]
HeLa EC50
272 μM
Compound: Nfx
Cytotoxicity against human HeLa cells measured after 24 hrs by crystal violet staining based assay
Cytotoxicity against human HeLa cells measured after 24 hrs by crystal violet staining based assay
[PMID: 27810595]
HeLa EC50
91.2 μM
Compound: 5
Cytotoxicity against human HeLa cells after 65 hrs by Alamar blue assay
Cytotoxicity against human HeLa cells after 65 hrs by Alamar blue assay
[PMID: 23281892]
HepG2 CC50
45.2 μM
Compound: Nifurtimox
Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 72 hrs by MTT assay
Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 72 hrs by MTT assay
[PMID: 32795774]
HepG2 CC50
45.2 μM
Compound: Nifurtimox
Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 72 hrs by MTT assay
Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 72 hrs by MTT assay
[PMID: 32652409]
IMR-90 EC50
> 25 μM
Compound: Nifurtimox
Cytotoxicity against human IMR90 cells after 44 to 46 hrs by CellTiter-Blue assay
Cytotoxicity against human IMR90 cells after 44 to 46 hrs by CellTiter-Blue assay
[PMID: 28119024]
J774 IC50
> 200 μM
Compound: S5
Cytotoxicity against J774.1 cell line after 48 hrs
Cytotoxicity against J774.1 cell line after 48 hrs
[PMID: 16516467]
J774 CC50
131.5 μM
Compound: Nfx
Cytotoxicity against mouse J774 cells incubated for 24 hrs by resazurin dye based fluorescence assay
Cytotoxicity against mouse J774 cells incubated for 24 hrs by resazurin dye based fluorescence assay
[PMID: 28648464]
J774 CC50
131.503 μM
Compound: NFX
Cytotoxicity against mouse J774 cells assessed as decrease in cell viability after 24 hrs by resazurin assay
Cytotoxicity against mouse J774 cells assessed as decrease in cell viability after 24 hrs by resazurin assay
[PMID: 27503677]
J774 EC50
150 μM
Compound: Nfx
Cytotoxicity against mouse J774 cells assessed as reduction in cell viability after 24 hrs by WST-1 assay
Cytotoxicity against mouse J774 cells assessed as reduction in cell viability after 24 hrs by WST-1 assay
[PMID: 31673311]
J774 IC50
280.48 μM
Compound: NFX
Cytotoxicity against mouse J774 cells after 48 hrs by MTT assay
Cytotoxicity against mouse J774 cells after 48 hrs by MTT assay
[PMID: 23816040]
J774 IC50
316 μM
Compound: Nifurtimox
Cytotoxicity against mouse J774 cells assessed as reduction in cell viability after 48 hrs by MTT assay
Cytotoxicity against mouse J774 cells assessed as reduction in cell viability after 48 hrs by MTT assay
[PMID: 28499168]
J774 IC50
316 μM
Compound: Nfx
Cytotoxicity against mouse J774 cells assessed as cell viability after 48 hrs by MTT assay
Cytotoxicity against mouse J774 cells assessed as cell viability after 48 hrs by MTT assay
[PMID: 25008454]
J774 IC50
316 μM
Compound: Nfx
Cytotoxicity against mouse J774 cells assessed as cell viability after 48 hrs by MTT assay
Cytotoxicity against mouse J774 cells assessed as cell viability after 48 hrs by MTT assay
[PMID: 23811257]
J774.2 CC50
164.2 μM
Compound: Nfx
Cytotoxicity against mouse J774.2 cells assessed as reduction in cell viability measured after 48 hrs by resazurin dye based assay
Cytotoxicity against mouse J774.2 cells assessed as reduction in cell viability measured after 48 hrs by resazurin dye based assay
[PMID: 34571489]
J774.A1 CC50
201.1 μM
Compound: Nfx
Cytotoxicity against mouse J774.A1 cells assessed as reduction in cell viability measured after 20 hrs by MTT assay
Cytotoxicity against mouse J774.A1 cells assessed as reduction in cell viability measured after 20 hrs by MTT assay
[PMID: 30784876]
L6 IC50
32 μM
Compound: Nifurtimox
Cytotoxicity against rat L6 cells after 6 days by resazurin based fluorescence assay
Cytotoxicity against rat L6 cells after 6 days by resazurin based fluorescence assay
[PMID: 27591008]
L6 IC50
32 μM
Compound: nifurtimox
Cytotoxicity against rat L6 cells after 6 days by resazurin staining based fluorescence plate reader assay
Cytotoxicity against rat L6 cells after 6 days by resazurin staining based fluorescence plate reader assay
[PMID: 26479031]
L6 IC50
68 μM
Compound: nifurtimox
Concentration causing cytotoxicity to 50% of L-6 rat skeletal myoblasts
Concentration causing cytotoxicity to 50% of L-6 rat skeletal myoblasts
[PMID: 16107157]
L6 CC50
78.2 μM
Compound: Nifurtimox
Cytotoxicity against rat L6 cells after 5 days by resazurin assay
Cytotoxicity against rat L6 cells after 5 days by resazurin assay
[PMID: 25089808]
L929 IC50
> 256 μM
Compound: Nifurtimox
Cytotoxicity against mouse NCTC-929 cells assessed as reduction in cell viability after 48 hrs by resazurin dye-based assay
Cytotoxicity against mouse NCTC-929 cells assessed as reduction in cell viability after 48 hrs by resazurin dye-based assay
[PMID: 28499168]
LLC-MK2 IC50
1.9 μM
Compound: Nfx
Antitrypanosomal activity against epimastigotes of Trypanosoma cruzi Y infected in LLC-MK2 cells assessed as parasite motility after 11 days
Antitrypanosomal activity against epimastigotes of Trypanosoma cruzi Y infected in LLC-MK2 cells assessed as parasite motility after 11 days
[PMID: 23167554]
LLC-MK2 CC50
2.7 μM
Compound: Nfx
Antitrypanosomal activity against Trypanosoma cruzi Y metacyclic trypomastigotes isolated from infected LLC-MK2 cells assessed as parasite viability after 24 hrs by neubauer chamber analysis
Antitrypanosomal activity against Trypanosoma cruzi Y metacyclic trypomastigotes isolated from infected LLC-MK2 cells assessed as parasite viability after 24 hrs by neubauer chamber analysis
[PMID: 24561675]
LLC-MK2 IC50
2.7 μM
Compound: Nfx
Antitrypanosomal activity against trypomastigotes of Trypanosoma cruzi Y infected in LLC-MK2 cells assessed as parasite motility after 24 hrs
Antitrypanosomal activity against trypomastigotes of Trypanosoma cruzi Y infected in LLC-MK2 cells assessed as parasite motility after 24 hrs
[PMID: 23167554]
MRC5 IC50
0.7 μM
Compound: Nifurtimox
Trypanocidal activity against nifurtimox-sensitive Trypanosoma cruzi Tulahuen CL2 infected in human MRC5 SV2 cells assessed as parasite growth inhibition after 168 hrs by beta-galactosidase assay
Trypanocidal activity against nifurtimox-sensitive Trypanosoma cruzi Tulahuen CL2 infected in human MRC5 SV2 cells assessed as parasite growth inhibition after 168 hrs by beta-galactosidase assay
[PMID: 25199582]
NIH3T3 IC50
3 μg/mL
Compound: nifurtimox
Antitrypanosomal activity against Trypanosoma cruzi Tulahuen infected in mouse 3T3 cells assessed as beta-galactosidase activity after 7 days by chagas bioassay
Antitrypanosomal activity against Trypanosoma cruzi Tulahuen infected in mouse 3T3 cells assessed as beta-galactosidase activity after 7 days by chagas bioassay
[PMID: 20441198]
RAW264.7 IC50
263.44 μM
Compound: Nifurtimox
Cytotoxicity against mouse RAW264.7 cells measured after 4 hrs by MTT assay
Cytotoxicity against mouse RAW264.7 cells measured after 4 hrs by MTT assay
[PMID: 27908757]
THP-1 IC50
> 100 μM
Compound: Nifurtimox
Cytotoxicity against human THP1 cells by AlamarBlue assay
Cytotoxicity against human THP1 cells by AlamarBlue assay
[PMID: 24119553]
THP-1 IC50
64.8 μM
Compound: nifurtimox
Cytotoxicity against human THP1 cells after 6 days by Alamar blue assay
Cytotoxicity against human THP1 cells after 6 days by Alamar blue assay
[PMID: 20028822]
U2OS EC50
0.34 μM
Compound: Nifurtimox
Antitrypanosomal activity against amastigote stage of Trypanosoma cruzi Y infected in human U2OS cells after 96 hrs by Draq5 staining-based high content screening assay
Antitrypanosomal activity against amastigote stage of Trypanosoma cruzi Y infected in human U2OS cells after 96 hrs by Draq5 staining-based high content screening assay
[PMID: 27318979]
U2OS EC50
1.26 μM
Compound: Nifurtimox
Antiparasitic activity against Trypanosoma cruzi Y trypomastigotes infected in rhesus monkey LLC-MK2 cells followed by re-infection of amastigotes in human U2OS cells assessed as reduction in parasite infection level after 96 hrs by draq5 staining based a
Antiparasitic activity against Trypanosoma cruzi Y trypomastigotes infected in rhesus monkey LLC-MK2 cells followed by re-infection of amastigotes in human U2OS cells assessed as reduction in parasite infection level after 96 hrs by draq5 staining based a
[PMID: 26774924]
U2OS CC50
26.8 μM
Compound: Nifurtimox
Cytotoxicity against human U2OS cells infected with amastigote stage of Trypanosoma cruzi Y assessed as decrease in number of cells after 96 hrs by Draq5 staining-based high content screening assay
Cytotoxicity against human U2OS cells infected with amastigote stage of Trypanosoma cruzi Y assessed as decrease in number of cells after 96 hrs by Draq5 staining-based high content screening assay
[PMID: 27318979]
V79 IC50
35 μM
Compound: Nifurtimox
Cytotoxicity against chinese hamster V79 cells after 6 days by Alamar blue assay
Cytotoxicity against chinese hamster V79 cells after 6 days by Alamar blue assay
[PMID: 20679506]
Vero IC50
0.24 μM
Compound: Nifurtimox
Antitrypanosomal activity against Trypanosoma cruzi clone Cl-Brener infected in african green monkey Vero cells assessed as growth inhibition after 3 days by luciferase reporter gene assay
Antitrypanosomal activity against Trypanosoma cruzi clone Cl-Brener infected in african green monkey Vero cells assessed as growth inhibition after 3 days by luciferase reporter gene assay
[PMID: 20679506]
Vero IC50
0.45 μM
Compound: Nfx
Antitrypanosomal activity against Trypanosoma cruzi Sylvio X-10 amastigotes infected in African green monkey Vero cells assessed as eradication of amastigotes after 72 hrs by Giemsa staining-based assay
Antitrypanosomal activity against Trypanosoma cruzi Sylvio X-10 amastigotes infected in African green monkey Vero cells assessed as eradication of amastigotes after 72 hrs by Giemsa staining-based assay
[PMID: 24749923]
Vero IC50
0.52 μM
Compound: Nifurtimox
Antitrypanosomal activity against Trypanosoma cruzi Tulahuen C4 in african green monkey Vero cells after 120 hrs
Antitrypanosomal activity against Trypanosoma cruzi Tulahuen C4 in african green monkey Vero cells after 120 hrs
[PMID: 18798609]
Vero IC50
1.4 μM
Compound: Nfx
Antiparasitic activity against Trypanosoma cruzi 320I04 intracellular amastigotes infected in african green monkey Vero cells after 72 hrs
Antiparasitic activity against Trypanosoma cruzi 320I04 intracellular amastigotes infected in african green monkey Vero cells after 72 hrs
[PMID: 19321339]
Vero IC50
1.6 μM
Compound: Nfx
Antiparasitic activity against amastigote stage of Trypanosoma cruzi infected in Vero cells assessed as parasite growth inhibition after 24 hrs by hemocytometery
Antiparasitic activity against amastigote stage of Trypanosoma cruzi infected in Vero cells assessed as parasite growth inhibition after 24 hrs by hemocytometery
[PMID: 25173828]
Vero IC50
10 μM
Compound: Nifurtimox
Antitrypanosomal activity against trypomastigote stage of Trypanosoma cruzi Dm28c infected in African green monkey Vero cells assessed as growth inhibition incubated for 5 to 7 days post infection measured after 24 hrs by MTT assay
Antitrypanosomal activity against trypomastigote stage of Trypanosoma cruzi Dm28c infected in African green monkey Vero cells assessed as growth inhibition incubated for 5 to 7 days post infection measured after 24 hrs by MTT assay
[PMID: 27908757]
Vero IC50
10 μM
Compound: nifurtimox
Antitrypanosomal activity against Trypanosoma cruzi Tulahuen C4 in vero cells assessed as intracellular growth inhibition of trypomastigote by beta-galactosidase reporter gene assay
Antitrypanosomal activity against Trypanosoma cruzi Tulahuen C4 in vero cells assessed as intracellular growth inhibition of trypomastigote by beta-galactosidase reporter gene assay
[PMID: 18715036]
Vero IC50
10.4 μM
Compound: Nfx
Trypanosomicidal activity against amastigote stage of Trypanosoma cruzi Tulahuen C4 transfected with beta-D-galactosidase infected in african green monkey Vero cells assessed as growth inhibition incubated 5 days prior to beta-D-galactopyranoside addition
Trypanosomicidal activity against amastigote stage of Trypanosoma cruzi Tulahuen C4 transfected with beta-D-galactosidase infected in african green monkey Vero cells assessed as growth inhibition incubated 5 days prior to beta-D-galactopyranoside addition
[PMID: 23202852]
Vero IC50
11 μM
Compound: Nifurtimox
Antitrypanosomal activity against Trypanosoma cruzi Tulahuen C4 infected in african green monkey Vero cells assessed as growth inhibition of trypomastigotes by beta-galactosidase reporter gene assay
Antitrypanosomal activity against Trypanosoma cruzi Tulahuen C4 infected in african green monkey Vero cells assessed as growth inhibition of trypomastigotes by beta-galactosidase reporter gene assay
[PMID: 17067146]
Vero CC50
113.6 μM
Compound: Nfx
Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay
Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay
[PMID: 19321339]
Vero IC50
115 μM
Compound: NFX
Cytotoxicity against African green monkey Vero cells after 72 hrs by MTT assay
Cytotoxicity against African green monkey Vero cells after 72 hrs by MTT assay
[PMID: 30344907]
Vero IC50
13.5 μM
Compound: Nifurtimox
Antiparasitic activity against Trypanosoma cruzi Tulahuen C4 infected african green monkey Vero cells expressing beta-galctosidase after 5 days
Antiparasitic activity against Trypanosoma cruzi Tulahuen C4 infected african green monkey Vero cells expressing beta-galctosidase after 5 days
[PMID: 20030365]
Vero CC50
163 μM
Compound: Nfx, Lampit
Cytotoxicity against African green monkey Vero cells after 24 hrs by MTT assay
Cytotoxicity against African green monkey Vero cells after 24 hrs by MTT assay
[PMID: 25899334]
Vero IC50
2.3 μM
Compound: Nfx
Antitrypanosomal activity against Trypanosoma cruzi 320I01 amastigotes infected in african green monkey Vero cells after 5 days by Giemsa staining
Antitrypanosomal activity against Trypanosoma cruzi 320I01 amastigotes infected in african green monkey Vero cells after 5 days by Giemsa staining
[PMID: 20627590]
Vero IC50
2.4 μM
Compound: Nfx
Antitrypanosomal activity against Trypanosoma cruzi Sylvio X-10 amastigotes infected in African green monkey Vero cells assessed as eradication of amastigotes after 72 hrs by Giemsa staining-based assay
Antitrypanosomal activity against Trypanosoma cruzi Sylvio X-10 amastigotes infected in African green monkey Vero cells assessed as eradication of amastigotes after 72 hrs by Giemsa staining-based assay
[PMID: 24749923]
Vero IC50
21.05 μM
Compound: Nifurtimox
Trypanocidal activity against epimastigote stage of Trypanosoma cruzi infected in african green monkey Vero cells assessed as cell viability after 24 hrs by MTT assay
Trypanocidal activity against epimastigote stage of Trypanosoma cruzi infected in african green monkey Vero cells assessed as cell viability after 24 hrs by MTT assay
[PMID: 25127463]
Vero CC50
32 μM
Compound: Nifurtimox
Cytotoxicity against African green monkey Vero cells incubated for 24 to 48 hrs by SRB assay
Cytotoxicity against African green monkey Vero cells incubated for 24 to 48 hrs by SRB assay
[PMID: 28645659]
Vero IC50
4 μM
Compound: Nfx
Antiparasitic activity against epimastigote stage of Trypanosoma cruzi infected in Vero cells assessed as parasite growth inhibition after 24 hrs by hemocytometery
Antiparasitic activity against epimastigote stage of Trypanosoma cruzi infected in Vero cells assessed as parasite growth inhibition after 24 hrs by hemocytometery
[PMID: 25173828]
Vero IC50
4.5 μM
Compound: Nifurtimox
Antitrypanosomal activity against Trypanosoma cruzi tulahuen C4 trypomastigotes infected in african green monkey Vero cells assessed as CPRG cleavage after 120 hrs by microplate reader
Antitrypanosomal activity against Trypanosoma cruzi tulahuen C4 trypomastigotes infected in african green monkey Vero cells assessed as CPRG cleavage after 120 hrs by microplate reader
[PMID: 20356752]
Vero IC50
4.8 μM
Compound: Nfx
Antiparasitic activity against Trypanosoma cruzi 320I04 epimastigotes infected in african green monkey Vero cells after 72 hrs
Antiparasitic activity against Trypanosoma cruzi 320I04 epimastigotes infected in african green monkey Vero cells after 72 hrs
[PMID: 19321339]
Vero IC50
411.13 μM
Compound: Nifurtimox
Cytotoxicity against african green monkey Vero cells by MTT assay
Cytotoxicity against african green monkey Vero cells by MTT assay
[PMID: 25127463]
Vero CC50
61.42 μM
Compound: Nfx
Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay
Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay
[PMID: 20627590]
Vero IC50
64.11 μM
Compound: Nifurtimox
Cytotoxicity against african green monkey Vero cells after 6 days by Alamar blue assay
Cytotoxicity against african green monkey Vero cells after 6 days by Alamar blue assay
[PMID: 20679506]
Vero IC50
80.1 μM
Compound: Nifurtimox
Cytotoxicity against african green monkey Vero cells by MTT assay
Cytotoxicity against african green monkey Vero cells by MTT assay
[PMID: 18798609]
Vero IC50
94 μM
Compound: Nifurtimox
Cytotoxicity against african green monkey Vero cells after 72 hrs by MTS assay
Cytotoxicity against african green monkey Vero cells after 72 hrs by MTS assay
[PMID: 20356752]
体外実験

Nifurtimox affects enzyme activity of lactate dehydrogenase (LDH). To differentiate if this effect is a result of a reduced LDH activity or a shift in pyruvate metabolism due to activation of PDH, the enzyme activity of LDH is determined after 4 h treatment with 50 μg/mL Nifurtimox. Compared to the untreated control, the LDH activity is significantly reduced for LA-N-1 (P=0.005), IMR-32 (P=0.009), LS (P=0.0035) and SK-N-SH (P=0.0065). Nifurtimox reduces cell viability and induces cell cycle arrest and apoptosis in neuroblastoma cells. To characterize the cytotoxic impacts of Nifurtimox on neuroblastoma, 4 cell lines are subjected to several experiments. Cell viability is reduced for all 4 neuroblastoma cell lines after 24 h incubation with 50 μg/mL to an average of 66%, 63%, 62% and 75% (LA-N-1, IMR-32 LS and SK-N-SH, respectively). The reduction is significant compared to the untreated control (P<0.01) and the vehicle control with DMSO (P<0.05) for all cell lines[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

臨床実験
分子量

287.30

分子式

C10H13N3O5S

CAS 番号
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=S1(CC(C)N(/N=C/C2=CC=C([N+]([O-])=O)O2)CC1)=O

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
溶剤 & 溶解度
体外: 

DMSO : 150 mg/mL (522.10 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.4807 mL 17.4034 mL 34.8068 mL
5 mM 0.6961 mL 3.4807 mL 6.9614 mL
10 mM 0.3481 mL 1.7403 mL 3.4807 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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一般には略語で表示されます:C1V1 = C2V2

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体積 (開始)

V1

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C2

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体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (8.70 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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g

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
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Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション

純度: 99.65%

参考文献
細胞実験
[1]

The Neuroblastoma cell lines IMR-32, LA-N-1 and SK-N-SH and the neuroblastoma cell line LS are grown in RPMI-1640 medium supplemented with 10% (v/v) fetal calf serum (FCS), 2 mM L-glutamine, 100 U/mL Penicillin and 100 µg/mL Streptomycin and incubated at 37°C, 5% CO2 and saturated humidity. To assess the cell viability after incubation with Nifurtimox at different concentrations (10 µg/mL up to 50 µg/mL or 34.8 µM to 174 µM, respectively in the supernatant growth medium) or the vehicle control with according concentrations, all neuroblastoma cell lines are subjected to an MTS assay. Stock solutions of MTS are made at 480 µM in sterile filtered deionized water and stored at -20°C. Cells are grown to approximately 50% confluency, treated with Nifurtimox, and incubated for 1 h with fresh media containing 12 µM MTS[1].

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参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.4807 mL 17.4034 mL 34.8068 mL 87.0171 mL
5 mM 0.6961 mL 3.4807 mL 6.9614 mL 17.4034 mL
10 mM 0.3481 mL 1.7403 mL 3.4807 mL 8.7017 mL
15 mM 0.2320 mL 1.1602 mL 2.3205 mL 5.8011 mL
20 mM 0.1740 mL 0.8702 mL 1.7403 mL 4.3509 mL
25 mM 0.1392 mL 0.6961 mL 1.3923 mL 3.4807 mL
30 mM 0.1160 mL 0.5801 mL 1.1602 mL 2.9006 mL
40 mM 0.0870 mL 0.4351 mL 0.8702 mL 2.1754 mL
50 mM 0.0696 mL 0.3481 mL 0.6961 mL 1.7403 mL
60 mM 0.0580 mL 0.2901 mL 0.5801 mL 1.4503 mL
80 mM 0.0435 mL 0.2175 mL 0.4351 mL 1.0877 mL
100 mM 0.0348 mL 0.1740 mL 0.3481 mL 0.8702 mL
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製品名:
Nifurtimox
製品番号:
HY-W040073
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