2. Stem Cell/Wnt
  3. Smo
  4. SAG

SAG is a potent Smoothened (Smo) receptor agonist (EC50=3 nM; Kd=59 nM). SAG activates the Hedgehog signaling pathway and counteracts Cyclopamine (HY-17024) inhibition of Smo.

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CAS 番号 : 912545-86-9

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 129 在庫あり
Solution
10 mM * 1 mL in DMSO USD 129 在庫あり
Solid
1 mg $60 在庫あり
5 mg $120 在庫あり
10 mg $150 在庫あり
25 mg $300 在庫あり
50 mg $450 在庫あり
100 mg $675 在庫あり
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カスタマーレビュー

Based on 37 publication(s) in Google Scholar

Other Forms of SAG:

SAG 関連抗体

Top Publications Citing Use of Products

顧客検証

RT-PCR
IF
WB

    SAG purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2024 Feb 15;22(1):123.  [Abstract]

    qPCR detecting expression alterations of IL-6, MIP-2, and E-selectin in RS218 infected hBMECs with or without SAG pre-treatment (at 10 μM).

    SAG purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2024 Feb 15;22(1):123.  [Abstract]

    Western blot detecting expression of E-selectin, KRAS and ERK1/2 phosphorylation in RS218 infected hBMECs with or without SAG pre-treatment (at 10 μM).

    SAG purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2024 Feb 15;22(1):123.  [Abstract]

    IF assays showing the E-selectin and KRAS expression in brains of mice challenged by RS218 with or without SAG (10 mg/kg, i.v.; single dose) pre-treatment. The E-selection and KRAS were stained in red. CD31 was specifically applied for labeling the micro-vessels in green. The cell nucleus was stained in blue with DAPI. Scale bar = 50 μm

    SAG purchased from MedChemExpress. Usage Cited in: Sci Adv. 2023 Jun 16;9(24):eadf6927.  [Abstract]

    Representative immunofluorescent images of TdTomato (tdTom) and brachyury (T) at E10.5 in trunk cross sections of indicated genotypes with or without small molecule agonist (SAG, 20 mg/kg; i.p.) treatment. Scale bar, 100 μm.

    SAG purchased from MedChemExpress. Usage Cited in: Cell Res. 2022 Mar;32(3):288-301.  [Abstract]

    SAG (5-200 nM)-induced Gli1 expression is independent of extracellular Ca2+ concentration. Sub-confluent NIH3T3 cells were serum-starved in 0.5% FCS overnight and switched to normal DMEM or Ca2+-free DMEM containing indicated concentration of SAG. Cells were harvested for RNA extraction after 6 hours.

    SAG purchased from MedChemExpress. Usage Cited in: Development. 2018 Sep 20;145(18). pii: dev164830.  [Abstract]

    WT MEF cells are treated with 100 nM SAG, 0.1mM ATP, or 0.1mM NHATP for 10 min (U). p-Creb, total Creb and Tubulin protein levels are detected by Western blotting.

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    SAG is a potent Smoothened (Smo) receptor agonist (EC50=3 nM; Kd=59 nM). SAG activates the Hedgehog signaling pathway and counteracts Cyclopamine (HY-17024) inhibition of Smo[1][2][3].

    IC50 & Target

    EC50: 3 nM (Smo)[1]
    Cellular Effect
    Cell Line Type Value Description References
    C3H 10T1/2 EC50
    0.13 μM
    Compound: SAG
    Agonist activity at Smo in mouse C3H10T1/2 cells assessed as induction of cell differentiation into osteoblast incubated for 6 days by alkaline phosphatase assay
    Agonist activity at Smo in mouse C3H10T1/2 cells assessed as induction of cell differentiation into osteoblast incubated for 6 days by alkaline phosphatase assay
    		[PMID: 27429255]
    HUVEC IC50
    22 μM
    Compound: 10a
    Cytotoxicity against HUVEC after 24 hrs by WST-1 assay
    Cytotoxicity against HUVEC after 24 hrs by WST-1 assay
    		[PMID: 22985958]
    Shh Light II EC50
    8.8 nM
    Compound: 10a
    Activation of Shh pathway in mouse Shh-Light II cells assessed as induction of gli1 expression after 48 hrs by renilla luminescence assay
    Activation of Shh pathway in mouse Shh-Light II cells assessed as induction of gli1 expression after 48 hrs by renilla luminescence assay
    		[PMID: 22985958]
    体外実験

    SAG (0.1 nM-100 μM; 30 h) induces firefly luciferase expression in Shh-LIGHT2 cells with an EC50 of 3 nM and then inhibits expression at higher concentrations[1].
    SAG (1-1000 nM; 1 h) competes for the binding of BODIPY-cyclopamine to Smo-expressing Cos-1 cells, yielding an apparent dissociation constant (Kd) of 59 nM for the SAG/Smo complex[1].
    SAG (100 nM) inhibits the inhibitory effect of ShhN-induced pathway activation by Robotnikinin[2].
    SAG (250 nM; 48 h) significantly increases SMO mRNA and protein expression in MDAMB231 cells[3].
    SAG (250 nM; 24 and 48 h) increases CAXII mRNA expression in MDAMB231 cells at 24h in normoxic and hypoxic conditions in MDAMB231 cells[3].
    SAG (250 nM; 24 h) increases MDAMB231 cells migration[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    SAG 関連抗体
    体内実験

    SAG (1.0 mM) induces more osteogenesis mainly at the defect borders and a significant increase in BV/TV at the eight-week timepoint in CD-1 mice[4].
    SAG (15-20 mg/kg; i.p.) induces pre-axial polydactyly prevalently in a dose-dependent manner in mice[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Pregnant C57BL/6J mice[5]
    Dosage: 15, 17, 20 mg/kg
    Administration: A single i.p.
    Result: Effective in ca. 80% of the embryos and increased Gli1 and Gli2 mRNA expression in the limb bud, with Gli1 mRNA being the most upregulated at the dose of 20 mg/kg.
    分子量

    490.06

    分子式

    C28H28ClN3OS
    CAS 番号
    Appearance

    Solid

    Color

    White to yellow

    SMILES

    O=C(C1=C(Cl)C2=CC=CC=C2S1)N([C@H]3CC[C@H](NC)CC3)CC4=CC=CC(C5=CC=NC=C5)=C4
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
    溶剤 & 溶解度
    体外: 

    DMSO : 100 mg/mL (204.06 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.0406 mL 10.2028 mL 20.4057 mL
    5 mM 0.4081 mL 2.0406 mL 4.0811 mL
    10 mM 0.2041 mL 1.0203 mL 2.0406 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

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    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 5 mg/mL (10.20 mM); Clear solution

      This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 5 mg/mL (10.20 mM); Clear solution

      This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

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    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 2 years; -20°C, 1 year (protect from light)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.79%

    COA (251 KB) HNMR (273 KB) CNMR (326 KB) RP-HPLC (152 KB) LCMS (94 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.0406 mL 10.2028 mL 20.4057 mL 51.0142 mL
    5 mM 0.4081 mL 2.0406 mL 4.0811 mL 10.2028 mL
    10 mM 0.2041 mL 1.0203 mL 2.0406 mL 5.1014 mL
    15 mM 0.1360 mL 0.6802 mL 1.3604 mL 3.4009 mL
    20 mM 0.1020 mL 0.5101 mL 1.0203 mL 2.5507 mL
    25 mM 0.0816 mL 0.4081 mL 0.8162 mL 2.0406 mL
    30 mM 0.0680 mL 0.3401 mL 0.6802 mL 1.7005 mL
    40 mM 0.0510 mL 0.2551 mL 0.5101 mL 1.2754 mL
    50 mM 0.0408 mL 0.2041 mL 0.4081 mL 1.0203 mL
    60 mM 0.0340 mL 0.1700 mL 0.3401 mL 0.8502 mL
    80 mM 0.0255 mL 0.1275 mL 0.2551 mL 0.6377 mL
    100 mM 0.0204 mL 0.1020 mL 0.2041 mL 0.5101 mL
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    SAG Related Classifications

    • Molarity Calculator

    • Dilution Calculator

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    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    SAG912545-86-9SmoSmoothenedInhibitorinhibitorinhibit

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    製品名:
    SAG
    製品番号:
    HY-12848
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