1. Stem Cell/Wnt
  2. Hedgehog

Cyclopamine (Synonyms: 11-Deoxojervine)

Cat. No.: HY-17024 Purity: >98.0%
Handling Instructions

Cyclopamine is a Hedgehog (Hh) pathway antagonist with IC50 of 46 nM in the Hh cell assay.

For research use only. We do not sell to patients.
Cyclopamine Chemical Structure

Cyclopamine Chemical Structure

CAS No. : 4449-51-8

Size Price Stock Quantity
5 mg USD 62 In-stock
10 mg USD 78 In-stock
50 mg USD 181 In-stock
100 mg USD 283 In-stock
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Customer Review

    Cyclopamine purchased from MCE. Usage Cited in: Life Sci. 2017 Dec 15;191:82-89.

    ORS cells are exposed to 3% oxygen for 48 h in presence or absence of Shh pathway inhibitor cyclopamine (5 μM) or GANT61 (10 μM). Immunofluorescence assay using anti-PCNA antibody is performed to detect the proliferative ORS cells (red). Cell nuclei are counterstained with DAPI (blue).

    Cyclopamine purchased from MCE. Usage Cited in: Int J Nanomedicine. 2017 Apr 20;12:3267-3280.

    The Smo inhibitor Cyclopamine suppresses the activity of Hedgehog–Gli1 signaling. The gene expression and protein production of Smo in MG63 osteoblasts after 7 days of incubation on the different titanium surfaces.

    Cyclopamine purchased from MCE. Usage Cited in: Neurochem Res. 2016 Apr;41(4):687-95.

    Western blot and RT-qPCR assays of BDNF protein and gene expressions in primary cortical neurons in different experimental conditions. Immunoblotting bands of BDNF protein.

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    • Biological Activity

    • Protocol

    • Technical Information

    • Purity & Documentation

    • References

    Description

    Cyclopamine is a Hedgehog (Hh) pathway antagonist with IC50 of 46 nM in the Hh cell assay.

    IC50 & Target

    IC50: 46 nM (Hedgehog, in Hh cell assay)[1]

    In Vitro

    Treatment with small molecule Hh inhibitors such as HhAntag and the natural product Cyclopamine, both binding to Smo, induces tumor remission in a genetic mouse model of medulloblastoma[1]. Cyclopamine is a Hedgehog (Hh) pathway antagonist. Cyclopamine suppresses cell growth. Cyclopamine (3 μM) suppression of Hh pathway activity and growth in digestive tract tumour cell lines correlates with expression of PTCHmRNA[2]. Cyclopamine is a steroidal alkaloid that inhibits Hh signalling through direct interaction with Smo[3].

    In Vivo

    Cyclopamine causes durable regression of xenograft tumors. Tumors in Cyclopamine-treated animals, regress completely by 12 days[2]. Cyclopamine (1.2 mg) treatment blocks tumour formation of human pancreatic adenocarcinoma cells after transplantation into nude mice[3].

    References
    Preparing Stock Solutions
    Concentration Volume Mass 1 mg 5 mg 10 mg
    1 mM 2.4294 mL 12.1471 mL 24.2943 mL
    5 mM 0.4859 mL 2.4294 mL 4.8589 mL
    10 mM 0.2429 mL 1.2147 mL 2.4294 mL
    Please refer to the solubility information to select the appropriate solvent.
    Cell Assay
    [2]

    Cyclopamine is dissolved in DMSO and stored, and then diluted with appropriate media before use[2].

    Cells are cultured in triplicate in 96-well plates in assay media to which 5E1 monoclonal antibody, ShhNp and/or Cyclopamine (3 μM) are added at 0 h at concentrations indicated in the main text. Viable cell mass is determined by optical density measurements at 490 nm (OD490) at 2 and 4 days using the CellTiter96 colorimetric assay. Relative growth is calculated as OD (day 4)-OD (day 2)/OD (day 2)[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [3]

    Cyclopamine is suspended in triolein:ethanol (4:1 v/v) (Mice)[3].

    Mice[3]
    A total of 0.1 mL Hanks’ balanced salt solution and matrigel (1:1) containing 2×106 cells is injected subcutaneously into CD-1 nude mice. Tumors are grown for 4 days to a minimum volume of 125 mm3; treatment is initiated simultaneously for all subjects. Mice are injected subcutaneously with vector alone (triolein:ethanol 4:1 v/v) or a Cyclopamine suspension (1.2 mg per mouse in triolein:ethanol 4:1 v/v) daily for 7 days. At the end of the treatment period, tumours are excised from mice, weighed and then fixed for 3 h at 4°C with 4% paraformaldehyde, embedded in paraffin wax and sectioned (6 µm). Apoptotic cells are identified by TUNEL using recombinant Tdt. Sections are then counterstained with eosin. Eight ×20-magnified fields from regions corresponding to the exterior, middle and interior of two control and two cyclopamine-treated tumours are chosen at random. We counted the number of TUNEL-positive nuclei manually. Haematoxylin/eosin staining is done. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
    Molecular Weight

    411.62

    Formula

    C₂₇H₄₁NO₂

    CAS No.

    4449-51-8

    SMILES

    O[[email protected]@H](CC[[email protected]@]12C)CC1=CC[[email protected]@]([H])([[email protected]@]2(CC3=C4C)[H])[[email protected]@]3(CC[[email protected]@]54O[[email protected]]6([H])C[[email protected]@H](CN[[email protected]]6([[email protected]]5C)[H])C)[H]

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Shipping

    Room temperature in continental US; may vary elsewhere

    Solvent & Solubility

    DMSO: 0.32 mg/mL (Need ultrasonic)

    * "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

    Purity: >98.0%

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    Inquiry Information

    Product Name:
    Cyclopamine
    Cat. No.:
    HY-17024
    Quantity: