1. Stem Cell/Wnt
  2. Smo
  3. SAG dihydrochloride

SAG dihydrochloride 

Cat. No.: HY-12848C
Handling Instructions

SAG dihydrochloride is a potent Smoothened (Smo) receptor agonist (EC50=3 nM; Kd=59 nM). SAG dihydrochloride activates the Hedgehog signaling pathway and counteracts Cyclopamine (HY-17024) inhibition of Smo.

For research use only. We do not sell to patients.

SAG dihydrochloride Chemical Structure

SAG dihydrochloride Chemical Structure

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 291 In-stock
Estimated Time of Arrival: December 31
1 mg USD 75 In-stock
Estimated Time of Arrival: December 31
5 mg USD 235 In-stock
Estimated Time of Arrival: December 31
10 mg USD 375 In-stock
Estimated Time of Arrival: December 31
50 mg USD 850 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

SAG dihydrochloride is a potent Smoothened (Smo) receptor agonist (EC50=3 nM; Kd=59 nM). SAG dihydrochloride activates the Hedgehog signaling pathway and counteracts Cyclopamine (HY-17024) inhibition of Smo[1][2][3].

IC50 & Target

EC50: 3 nM (Smo)[1]

In Vitro

SAG (0.1 nM-100 μM; 30 h) induces firefly luciferase expression in Shh-LIGHT2 cells with an EC50 of 3 nM and then inhibits expression at higher concentrations[1].
SAG (1-1000 nM; 1 h) competes for the binding of BODIPY-cyclopamine to Smo-expressing Cos-1 cells, yielding an apparent dissociation constant (Kd) of 59 nM for the SAG/Smo complex[1].
SAG (100 nM) inhibits the inhibitory effect of ShhN-induced pathway activation by Robotnikinin[2].
SAG (250 nM; 48 h) significantly increases SMO mRNA and protein expression in MDAMB231 cells[3].
SAG (250 nM; 24 and 48 h) increases CAXII mRNA expression in MDAMB231 cells at 24h in normoxic and hypoxic conditions in MDAMB231 cells[3].
SAG (250 nM; 24 h) increases MDAMB231 cells migration[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

SAG (1.0 mM) induces more osteogenesis mainly at the defect borders and a significant increase in BV/TV at the eight week timepoint in CD-1 mice[4].
SAG (15-20 mg/kg; i.p.) induces pre-axial polydactyly prevalently in a dose-dependent manner in mice[5].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Pregnant C57BL/6J mice[5]
Dosage: 15, 17, 20 mg/kg
Administration: A single i.p.
Result: Effective in ca. 80% of the embryos and increased Gli1 and Gli2 mRNA expression in the limb bud, with Gli1 mRNA being the most upregulated at the dose of 20 mg/kg.
Molecular Weight

562.98

Formula

C₂₈H₃₀Cl₃N₃OS

SMILES

O=C(C1=C(Cl)C2=CC=CC=C2S1)N([[email protected]]3CC[[email protected]](NC)CC3)CC4=CC=CC(C5=CC=NC=C5)=C4.[H]Cl.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
References
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Keywords:

SAGSmoSmoothenedSMOHhpathwayInhibitorinhibitorinhibit

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Product Name:
SAG dihydrochloride
Cat. No.:
HY-12848C
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