1. Stem Cell/Wnt
  2. Smo
  3. SAG dihydrochloride

SAG dihydrochloride 

Cat. No.: HY-12848C
Handling Instructions

SAG dihydrochloride is a potent Smoothened (Smo) receptor agonist (EC50=3 nM; Kd=59 nM). SAG dihydrochloride activates the Hedgehog signaling pathway and counteracts Cyclopamine (HY-17024) inhibition of Smo.

For research use only. We do not sell to patients.

SAG dihydrochloride Chemical Structure

SAG dihydrochloride Chemical Structure

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 291 In-stock
Estimated Time of Arrival: December 31
1 mg USD 75 In-stock
Estimated Time of Arrival: December 31
5 mg USD 235 In-stock
Estimated Time of Arrival: December 31
10 mg USD 375 In-stock
Estimated Time of Arrival: December 31
50 mg USD 850 In-stock
Estimated Time of Arrival: December 31
100 mg   Get quote  
200 mg   Get quote  

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Customer Review

Based on 7 publication(s) in Google Scholar

Other Forms of SAG dihydrochloride:

Top Publications Citing Use of Products

    SAG dihydrochloride purchased from MCE. Usage Cited in: Development. 2018 Sep 20;145(18). pii: dev164830.

    WT MEF cells are treated with 100 nM SAG, 0.1mM ATP, or 0.1mM NHATP for 10 min (U). p-Creb, total Creb and Tubulin protein levels are detected by Western blotting.
    • Biological Activity

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    • References

    • Customer Review

    Description

    SAG dihydrochloride is a potent Smoothened (Smo) receptor agonist (EC50=3 nM; Kd=59 nM). SAG dihydrochloride activates the Hedgehog signaling pathway and counteracts Cyclopamine (HY-17024) inhibition of Smo[1][2][3].

    IC50 & Target

    EC50: 3 nM (Smo)[1]

    In Vitro

    SAG (0.1 nM-100 μM; 30 h) induces firefly luciferase expression in Shh-LIGHT2 cells with an EC50 of 3 nM and then inhibits expression at higher concentrations[1].
    SAG (1-1000 nM; 1 h) competes for the binding of BODIPY-cyclopamine to Smo-expressing Cos-1 cells, yielding an apparent dissociation constant (Kd) of 59 nM for the SAG/Smo complex[1].
    SAG (100 nM) inhibits the inhibitory effect of ShhN-induced pathway activation by Robotnikinin[2].
    SAG (250 nM; 48 h) significantly increases SMO mRNA and protein expression in MDAMB231 cells[3].
    SAG (250 nM; 24 and 48 h) increases CAXII mRNA expression in MDAMB231 cells at 24h in normoxic and hypoxic conditions in MDAMB231 cells[3].
    SAG (250 nM; 24 h) increases MDAMB231 cells migration[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    In Vivo

    SAG (1.0 mM) induces more osteogenesis mainly at the defect borders and a significant increase in BV/TV at the eight week timepoint in CD-1 mice[4].
    SAG (15-20 mg/kg; i.p.) induces pre-axial polydactyly prevalently in a dose-dependent manner in mice[5].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Pregnant C57BL/6J mice[5]
    Dosage: 15, 17, 20 mg/kg
    Administration: A single i.p.
    Result: Effective in ca. 80% of the embryos and increased Gli1 and Gli2 mRNA expression in the limb bud, with Gli1 mRNA being the most upregulated at the dose of 20 mg/kg.
    Molecular Weight

    562.98

    Formula

    C₂₈H₃₀Cl₃N₃OS

    SMILES

    O=C(C1=C(Cl)C2=CC=CC=C2S1)N([[email protected]]3CC[[email protected]](NC)CC3)CC4=CC=CC(C5=CC=NC=C5)=C4.[H]Cl.[H]Cl

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    H2O : 100 mg/mL (177.63 mM; Need ultrasonic)

    DMSO : 33.33 mg/mL (59.20 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.7763 mL 8.8813 mL 17.7626 mL
    5 mM 0.3553 mL 1.7763 mL 3.5525 mL
    10 mM 0.1776 mL 0.8881 mL 1.7763 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (4.44 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.5 mg/mL (4.44 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (4.44 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References
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    Keywords:

    SAGSmoSmoothenedSMOHhpathwayInhibitorinhibitorinhibit

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    Product Name:
    SAG dihydrochloride
    Cat. No.:
    HY-12848C
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