1. Stem Cell/Wnt
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  3. SAG

SAG 

Cat. No.: HY-12848 Purity: 99.88%
Handling Instructions

SAG is a potent Smoothened (Smo) receptor agonist (EC50=3 nM; Kd=59 nM). SAG activates the Hedgehog signaling pathway and counteracts Cyclopamine (HY-17024) inhibition of Smo.

For research use only. We do not sell to patients.

SAG Chemical Structure

SAG Chemical Structure

CAS No. : 912545-86-9

Size Price Stock Quantity
Solution
10 mM * 1 mL in DMSO USD 129 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 129 In-stock
Estimated Time of Arrival: December 31
Solid
1 mg USD 60 In-stock
Estimated Time of Arrival: December 31
5 mg USD 120 In-stock
Estimated Time of Arrival: December 31
10 mg USD 190 In-stock
Estimated Time of Arrival: December 31
25 mg USD 350 In-stock
Estimated Time of Arrival: December 31
50 mg USD 490 In-stock
Estimated Time of Arrival: December 31
100 mg   Get quote  
200 mg   Get quote  

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Customer Review

Based on 10 publication(s) in Google Scholar

Other Forms of SAG:

Top Publications Citing Use of Products

    SAG purchased from MCE. Usage Cited in: Development. 2018 Sep 20;145(18). pii: dev164830.

    WT MEF cells are treated with 100 nM SAG, 0.1mM ATP, or 0.1mM NHATP for 10 min (U). p-Creb, total Creb and Tubulin protein levels are detected by Western blotting.
    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    SAG is a potent Smoothened (Smo) receptor agonist (EC50=3 nM; Kd=59 nM). SAG activates the Hedgehog signaling pathway and counteracts Cyclopamine (HY-17024) inhibition of Smo[1][2][3].

    IC50 & Target

    EC50: 3 nM (Smo)[1]

    In Vitro

    SAG (0.1 nM-100 μM; 30 h) induces firefly luciferase expression in Shh-LIGHT2 cells with an EC50 of 3 nM and then inhibits expression at higher concentrations[1].
    SAG (1-1000 nM; 1 h) competes for the binding of BODIPY-cyclopamine to Smo-expressing Cos-1 cells, yielding an apparent dissociation constant (Kd) of 59 nM for the SAG/Smo complex[1].
    SAG (100 nM) inhibits the inhibitory effect of ShhN-induced pathway activation by Robotnikinin[2].
    SAG (250 nM; 48 h) significantly increases SMO mRNA and protein expression in MDAMB231 cells[3].
    SAG (250 nM; 24 and 48 h) increases CAXII mRNA expression in MDAMB231 cells at 24h in normoxic and hypoxic conditions in MDAMB231 cells[3].
    SAG (250 nM; 24 h) increases MDAMB231 cells migration[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    In Vivo

    SAG (1.0 mM) induces more osteogenesis mainly at the defect borders and a significant increase in BV/TV at the eight week timepoint in CD-1 mice[4].
    SAG (15-20 mg/kg; i.p.) induces pre-axial polydactyly prevalently in a dose-dependent manner in mice[5].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Pregnant C57BL/6J mice[5]
    Dosage: 15, 17, 20 mg/kg
    Administration: A single i.p.
    Result: Effective in ca. 80% of the embryos and increased Gli1 and Gli2 mRNA expression in the limb bud, with Gli1 mRNA being the most upregulated at the dose of 20 mg/kg.
    Molecular Weight

    490.06

    Formula

    C₂₈H₂₈ClN₃OS

    CAS No.
    SMILES

    O=C(C1=C(Cl)C2=CC=CC=C2S1)N([[email protected]]3CC[[email protected]](NC)CC3)CC4=CC=CC(C5=CC=NC=C5)=C4

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 38 mg/mL (77.54 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.0406 mL 10.2028 mL 20.4057 mL
    5 mM 0.4081 mL 2.0406 mL 4.0811 mL
    10 mM 0.2041 mL 1.0203 mL 2.0406 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 10 mg/mL (20.41 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 10 mg/mL (20.41 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 10 mg/mL (20.41 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References

    Purity: 99.88%

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    Keywords:

    SAGSmoSmoothenedInhibitorinhibitorinhibit

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    Cat. No.:
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