1. Stem Cell/Wnt
  2. Smo

SAG hydrochloride 

Cat. No.: HY-12848B
Handling Instructions

SAG (hydrochloride) is a potent Smo receptor agonist, and activates the Hedgehog signaling pathway, with a Kd of 59 nM.

For research use only. We do not sell to patients.

SAG hydrochloride Chemical Structure

SAG hydrochloride Chemical Structure

CAS No. : 2095432-58-7

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 139 In-stock
Estimated Time of Arrival: December 31
1 mg USD 60 In-stock
Estimated Time of Arrival: December 31
5 mg USD 120 In-stock
Estimated Time of Arrival: December 31
10 mg USD 190 In-stock
Estimated Time of Arrival: December 31
25 mg USD 350 In-stock
Estimated Time of Arrival: December 31
50 mg USD 490 In-stock
Estimated Time of Arrival: December 31
100 mg   Get quote  
200 mg   Get quote  

* Please select Quantity before adding items.

Other Forms of SAG hydrochloride:

    SAG hydrochloride purchased from MCE. Usage Cited in: Development. 2018 Aug 15. pii: dev.164830.

    WT MEF cells are treated with 100 nM SAG, 0.1mM ATP, or 0.1mM NHATP for 10 min (U). p-Creb, total Creb and Tubulin protein levels are detected by Western blotting.

    Featured Recommendations

    Related Screening Libraries:

    • Biological Activity

    • Technical Information

    • Purity & Documentation

    • References

    Description

    SAG (hydrochloride) is a potent Smo receptor agonist, and activates the Hedgehog signaling pathway, with a Kd of 59 nM.

    IC50 & Target

    Kd: 59 nM (Smo)[1].

    In Vitro

    SAG (hydrochloride) acts downstream of Ptch1 in the Hh pathway and counteracts cyclopamine inhibition of Smo. SAG induces firefly luciferase expression in Shh-LIGHT2 cells with an EC50 of 3 nM and then inhibits expression at higher concentrations. In Smo-expressing Cos-1 cells, SAG yields an apparent dissociation constant (KD) of 59 nM for the SAG/Smo complex[1]. SAG and purmorphamine verride the inhibitory effect of robotnikinin since Smo functions downstream of Shh/Ptc1[2].

    In Vivo

    In CD-1 mice, SAG (1.0 mM) or NELL-1 (600 μg/ml) alone results in increased bone formation at 4 and 8 weeks, but significantly greater bone formation with both components combined (SAG + NELL-1). The combination of the two compounds exhibits a significant increase in new bone formation, accompanied by increased defect vascularization[3]. SAG (15, 17, or 20 mg/kg, i.p.) induces pre-axial polydactyly prevalently. The highest SAG dose is effective in ca. 80% of the embryos and increased Gli1 and Gli2 mRNA expression in the limb bud, with Gli1 mRNA being the most upregulated[4].

    Solvent & Solubility
    In Vitro: 

    10 mM in DMSO

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.8993 mL 9.4963 mL 18.9926 mL
    5 mM 0.3799 mL 1.8993 mL 3.7985 mL
    10 mM 0.1899 mL 0.9496 mL 1.8993 mL
    *Please refer to the solubility information to select the appropriate solvent.
    References
    Molecular Weight

    526.52

    Formula

    C₂₈H₂₉Cl₂N₃OS

    CAS No.

    2095432-58-7

    SMILES

    O=C(C1=C(Cl)C2=CC=CC=C2S1)N([[email protected]]3CC[[email protected]](NC)CC3)CC4=CC=CC(C5=CC=NC=C5)=C4.Cl

    Storage

    Please store the product under the recommended conditions in the Certificate of Analysis.

    Shipping

    Room temperature in continental US; may vary elsewhere

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass   Concentration   Volume   Molecular Weight *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
    C1   V1   C2   V2

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product name

     

    Salutation

    Applicant name *

     

    Email address *

    Phone number *

     

    Organization name *

    Country or Region *

     

    Requested quantity *

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    SAG hydrochloride
    Cat. No.:
    HY-12848B
    Quantity:

    SAG hydrochloride

    Cat. No.: HY-12848B