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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-D1599
    Rhodamine 6G hydrazide
    		98.25%
    Rhodamine 6G hydrazide (R6GH) is a fluorescent dye. Rhodamine 6G hydrazide can be used in selective colorimetric and fluorescent sensing.
    Rhodamine 6G hydrazide
  • HY-127049
    Arachidonic acid-biotin
    		98.71%
    Arachidonic acid-Biotin is a biotin-labeled Arachidonic acid (HY-109590) that can be used to detect complexes of arachidonic acid with protein binding partners such as fatty acid binding proteins (FABPs).
    Arachidonic acid-biotin
  • HY-DY1030
    RhoNox-1 (solution)
    RhoNox-1 (solution) is a fluorescent probe for the specific detection of divalent iron ions, and when RhoNox-1 reacts with Fe2+. RhoNox-1 can generate an irreversible orange (red) fluorescent product (Ex/Em：540/575 nm). FeRhoNox-1 can enter the cell well, suitable for the detection of Fe2+ in living cells, and tends to be localized in the Golgi apparatus.
    Solvent and concentration: DMSO: 5 mM
    RhoNox-1 (solution)
  • HY-DY1026
    ER-Tracker Red (solution)
    ER-Tracker dye is a derivative of BODIPY series dyes coupled with Glibenclamide (HY-15206) , highly selective binding to the endoplasmic reticulum, non-toxic to cells at low concentrations, this type of dye is an environmentally sensitive probe, and formaldehyde treatment can still retain part of the fluorescence, with high fluorescence life, good extinction coefficient and other characteristics. Glibenclamide is an atp-dependent K+ channel blocker (Kir6, KATP) and CFTR Cl-channel blocker that binds in the endoplasmic reticulum. ER-Tracker is not suitable for staining cells after fixation. Ex/Em=587/615 nm.
    Solvent and concentration: DMSO: 1 mM
    ER-Tracker Red (solution)
  • HY-156305
    Coumarin-C2-exo-BCN
    Coumarin-C2-exoBCN is a dye derivative of Coumarin (HY-N0709). Coumarin-C2-exoBCN contains the lyophilic bidentate macrocyclic ligand exo-BCN, which can further synthesize macrocyclic complexes. In click chemistry, exo-BCN can react with molecules containing azide groups to generate stable triazoles under catalyst-free conditions.
    Coumarin-C2-exo-BCN
  • HY-D1629
    Calcium Orange AM
    		98%
    Calcium Orange AM is an intracellular calcium reporter. Specific fluorescence can be detected when free calcium binds to Calcium Orange AM (Ex/Em=549/576 nm). Calcium Orange AM does not enter the vacuoles and does not compartmentalize into acidic vesicles.
    Calcium Orange AM
  • HY-D0983
    Trypan red
    Trypan red is a vital stain.
    Trypan red
  • HY-126395
    Patent Blue V calcium salt
    		99.99%
    Patent Blue V calcium salt is a triarylmethane dye used for tissue staining and lymphatic tracing, mainly applied by topical injection or eye drops. Patent Blue V calcium salt has affinity for specific tissues (such as corneal endothelium, lymphatic system), and stains the target structure by adsorption or binding, assisting in precise operation during surgery. Patent Blue V calcium salt is mainly used in ophthalmic surgery (such as graft staining for Descemet's membrane endothelial keratoplasty) and lymphatic drainage localization for sentinel lymph node biopsy of tumors.
    Patent Blue V calcium salt
  • HY-P4406
    Abz-AGLA-Nba
    		99.71%
    Abz-AGLA-Nba is a fluorogenic substrate for the determination of protease activity. Abz-AGLA-Nba is hydrolyzed to release aminoacyl benzimide (Abz-AGLA) and 2-naphthylaminoacyl (Nba). The product Abz-AGLA produced by this hydrolysis reaction is fluorescent under ultraviolet light and can emit a fluorescent signal.
    Abz-AGLA-Nba
  • HY-W110927
    Alizarin Red S Indicator (4.3-6.3), IND
    Alizarin red S indicator (C.I. 58005) is a reductively active (quinone-based) anthraquinone dye that forms complexes with metal ions (such as zirconium, calcium) or boric acid to label calcium deposition and perform electrochemical sensing functions. Alizarin Red S undergoes reversible redox reactions (for electrochemical detection) and irreversible chelation (for bone staining). Alizarin Red S is mainly used in bone metabolism research (labeling mineralized tissue), sugar detection (boric acid-sugar competition system) and metal ion sensing (such as zirconium ion detection)， and can be used in osteoporosis and metabolic disease research.
    Alizarin Red S Indicator (4.3-6.3), IND
  • HY-D0853A
    DiAzKs hydrochloride
    		98.0%
    DiAzKs (H-L-Photo-lysine) hydrochloride is a diazirine-containing lysine amino acid and is a photo-cross-linker. DiAzKs hydrochloride can site-selective incorporated into proteins and is used to crosslink protein-protein interactions in vitro and in living cells. DiAzKs hydrochloride acts as a UV light-activated photo-crosslinking probe.
    DiAzKs hydrochloride
  • HY-D1446
    3′,6′-Bis(allyloxy)-Fluoran
    		98.05%
    3′,6′-Bis(allyloxy)-Fluoran is a multifunctional dye. Dyes are important tools in biological experiments. They can help researchers observe and analyze cell structures, track biomolecules, evaluate cell functions, distinguish cell types, detect biomolecules, study tissue pathology and monitor microorganisms. Their applications range from basic scientific research to clinical A wide range of diagnostics. Dyes are also widely used in traditional fields such as textile dyeing, as well as in emerging fields such as functional textile processing, food pigments and dye-sensitized solar cells.
    3′,6′-Bis(allyloxy)-Fluoran
  • HY-D1266
    RB-OPD
    		99.24%
    RB-OPD (NO-red) is a o-phenylenediamine (OPD)-locked rhodamine nitric oxide (NO) fluorescent probe with great sensitivity and selectivity (λex=550 nm, λem=590 nm).
    RB-OPD
  • HY-115749
    D-Luciferin 6′-methyl ether
    		99.55%
    D-Luciferin 6′-methyl ether (6′-Methoxyluciferin; compound 19a) is a potent luciferase from the North American firefly Photinus pyralis (PpyLuc) inhibitor with an IC50 of 0.1 µM. D-Luciferin 6′-methyl ether, a D-luciferin analog, shows non-specific interactions at ATP- and luciferin-binding sites of the PpyLuc active site.
    D-Luciferin 6′-methyl ether
  • HY-D2161A
    AF 594 azide triethylamine
    AF 594 azide (triethylamine) is an azide derivative of the red fluorescent dye AF 594, which has high fluorescence quantum yield and high photostability (maximum absorption wavelength of 586 nm, maximum emission wavelength of 613 nm). AF 594 azide (triethylamine) forms stable adducts by reaction of the azide group with alkynyl derivatives (terminal alkynes and cyclooctyne).
    AF 594 azide triethylamine
  • HY-W010947
    4-Methylumbelliferyl palmitate
    		99.23%
    4-Methylumbelliferyl palmitate is an excellent fluorophore for measuring acid lipase in human leukocytes. Acidity and solvent have important influence on its fluorescence. 4-Methylumbelliferyl palmitate exists mainly as neutral molecular form which can be produced strong fluorescence at 445 nm in near neutral aqueous solutions, and exist mainly as anion form which can be produced stronger fluorescence at 445 nm in weak alkaline solutions.
    4-Methylumbelliferyl palmitate
  • HY-P2491
    Atrial Natriuretic Peptide (1-28), human, porcine, Biotin-labeled
    		99.43%
    Atrial Natriuretic Peptide (1-28), human, porcine, Biotin-labeled, one of three mammalian natriuretic peptides (NPs), has endocrine effects on fluid homeostasis and blood pressure. Atrial Natriuretic Peptide has the potential for cardiovascular diseases research.
    Atrial Natriuretic Peptide (1-28), human, porcine, Biotin-labeled
  • HY-151708
    CalFluor 488 Azide
    CalFluor 488 Azide is a water-soluble fluorogenic azide probe. CalFluor 488 Azide is activated by Cu-catalyzed or metal-free click reaction. CalFluor 488 Azide is not fluorescent until it is reacted with alkynes. It contains an azide group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing alkyne groups. It can also undergo ring strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing DBCO or BCN groups.
    CalFluor 488 Azide
  • HY-D1075
    1,1',3,3,3',3'-Hexamethylindodicarbocyanine iodide
    		99.73%
    1,1',3,3,3',3'-Hexamethylindodicarbocyanine iodide is a carbocyanine dye that stains mitochondria of live cells.
    1,1',3,3,3',3'-Hexamethylindodicarbocyanine iodide
  • HY-DY1046
    Green CMFDA (solution)
    Green CMFDA (solution) is a cell-permeable fluorescent probe with Em of 514 nm and Ex of 485 nm and can be used as a cell tracer. Green CMFDA can be cleaved by non-specific esterases common in living cells, producing a fluorescent compound, fluorescein, visible using a fluorescent microscope.
    Solvent and concentration: DMSO: 10 mM
    Green CMFDA (solution)
Cat. No. Product Name / Synonyms Application Reactivity