Patent Blue V calcium salt 

Patent Blue V calcium salt is a triarylmethane dye used for tissue staining and lymphatic tracing, mainly applied by topical injection or eye drops. Patent Blue V calcium salt has affinity for specific tissues (such as corneal endothelium, lymphatic system), and stains the target structure by adsorption or binding, assisting in precise operation during surgery. Patent Blue V calcium salt is mainly used in ophthalmic surgery (such as graft staining for Descemet's membrane endothelial keratoplasty) and lymphatic drainage localization for sentinel lymph node biopsy of tumors^[1][2].

Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Patent Blue V (2.5%; 10 minutes) can maintain staining for more than 25 minutes in Descemet membrane endothelial keratoplasty (DMEK) graft staining experiments. Patent Blue V has a slightly lower staining intensity than Trypan blue (HY-D0970) and no significant difference in endothelial cell loss rate, making it a potential alternative dye to Trypan blue^[1].
Patent Blue V staining results can be detected by UV-visible spectrophotometry or fluorescence spectroscopy, and its maximum absorption wavelength is about 630 nm. The specific experimental steps are as follows^[2]:
1. Sample preparation: Dissolve Patent Blue V calcium salt in physiological saline or balanced salt solution (BSS) to prepare stock solution, and use a serial dilution method (such as 1/2 gradient) to prepare a concentration gradient solution.
2. Instrument calibration: Use a UV-visible spectrophotometer (such as Unicam UV/visible spectrophotometer), calibrate the baseline with a blank solvent (such as BSS), set the scan range to 326-700 nm, and the scan interval to 1 nm.
3. Spectral scanning: Take 100 μL of each concentration solution and inject it into the quartz cuvette, scan the absorption spectrum in the range of 326-700 nm, and record the absorbance (A) value. The typical absorption peak appears near 630 nm, confirming the maximum absorption wavelength.
4. Data verification: Compare the absorption curves of solutions of different concentrations to verify that the peak at 630 nm increases with increasing concentration, ensuring that there is no interference from other impurities.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

580.72

C₂₇H₃₂N₂O₇S₂.1/2Ca

3536-49-0

Solid

Brown to black

CC/[N+](CC)=C1C=C/C(C=C/1)=C(C2=CC=C(N(CC)CC)C=C2)\C3=CC(O)=C(S(=O)([O-])=O)C=C3S(=O)([O-])=O.[Ca+2].[1/2]

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Shin-Yi Chen, et al. Patent Blue V as an Alternative Stain for DMEK Grafts: Safety, Stain Retention, and Feasibility. Cornea. 2019 Oct;38(10):1322-1327.  [Content Brief]

  [2]. McTaggart MP, et al. Effect of Patent Blue V(®) dye on sample interference indices on the Abbott Architect. Ann Clin Biochem. 2012 Sep;49(Pt 5):510-1.  [Content Brief]

  [3]. Scherer K, et al. Anaphylaxis to isosulfan blue and cross-reactivity to patent blue V: case report and review of the nomenclature of vital blue dyes. Ann Allergy Asthma Immunol. 2006 Mar;96(3):497-500.  [Content Brief]