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  3. Fluorescent Dye
  4. Fast Green FCF

Fast Green FCF  (Synonyms: FD&C Green No. 3; Food green 3; C.I. 42053)

Cat. No.: HY-D0914 Purity: 97.37%
COA Handling Instructions

Fast Green FCF is a sea green triarylmethane food dye.

For research use only. We do not sell to patients.

Fast Green FCF Chemical Structure

Fast Green FCF Chemical Structure

CAS No. : 2353-45-9

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in Water
ready for reconstitution
 USD 55 In-stock
Solution
10 mM * 1 mL in Water USD 55 In-stock
Solid
500 mg USD 50 In-stock
1 g USD 60 In-stock
5 g   Get quote  
10 g   Get quote  

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This product is a controlled substance and not for sale in your territory.

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Based on 1 publication(s) in Google Scholar

Fast Green FCF Related Antibodies

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Description

Fast Green FCF is a sea green triarylmethane food dye. Fast Green FCF is used as a quantitative stain for histones at alkaline pH after acid extraction of DNA. It is also used as a protein stain in electrophoresis. Its absorption maximum is at 625 nm.

Molecular Weight

808.85

Appearance

Solid

Formula

C37H34N2Na2O10S3
CAS No.
Emission (Em)

522
Excitation (Ex)

486
SMILES

O=S(C1=CC(C/[N+](CC)=C2C=C/C(C=C/2)=C(C3=CC=C(N(CC)CC4=CC=CC(S(=O)(O[Na])=O)=C4)C=C3)\C5=CC=C(O)C=C5S(=O)(O[Na])=O)=CC=C1)([O-])=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

H2O : 10 mg/mL (12.36 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2363 mL 6.1816 mL 12.3632 mL
5 mM 0.2473 mL 1.2363 mL 2.4726 mL
10 mM 0.1236 mL 0.6182 mL 1.2363 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  PBS

    Solubility: 4.55 mg/mL (5.63 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

*All of the co-solvents are available by MedChemExpress (MCE).
Purity & Documentation

Purity: 97.37%

COA (259 KB) HNMR (320 KB) LCMS (96 KB)

Handling Instructions (2659 KB)
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Fast Green FCF Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

Fast Green FCF2353-45-9FD&C Green No. 3 Food green 3 C.I. 42053Food green3Food green 3Food green-3Fluorescent DyeInhibitorinhibitorinhibit

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