Congo Red
Based on 4 publication(s) in Google Scholar
Congo Red is an azo dye. Congo Red (CR) binding been used as a diagnostic test for the presence of amyloid in tissue sections.
For research use only. We do not sell to patients.
- Purity: 95.0%
- CAS No.: 573-58-0
- Formula: C32H22N6Na2O6S2
- Molecular Weight:696.66
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Congo Red
More
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| PC-12 | ED50 |
37.5 μg/mL
Compound: 10
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Protection against beta-amyloid (25 to 35) insult in rat PC12 cells assessed as viable cells after 24 hrs by MTT assay
Protection against beta-amyloid (25 to 35) insult in rat PC12 cells assessed as viable cells after 24 hrs by MTT assay
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[PMID: 12350137] |
| PC-12 | ED50 |
39.2 μg/mL
Compound: 10
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Protection against beta-amyloid (1 to 42) insult in rat PC12 cells assessed as viable cells after 24 hrs by MTT assay
Protection against beta-amyloid (1 to 42) insult in rat PC12 cells assessed as viable cells after 24 hrs by MTT assay
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[PMID: 12350137] |
Congo Red histochemical stain may serve as a simple screening tool for investigating if the aggregates in mutant cells have misfolded β-pleated sheet secondary structures. Congo Red histochemical dye has the ability to bind specifically to crossed β-pleated sheet structures. Wild-type HSPB1 should maintain protein homeostasis by binding proteins in non-native conformations, thereby preventing substrate aggregation. The T139M mutant, however, fails in this function and results in an accumulation of misfolded proteins, which are targeted by Congo Red for intercalating between the β-pleated sheet structures. Congo Red histochemical stain may serve as a simple tool to investigate if the aggregates in mutant cells have misfolded β-pleated sheet secondary structures[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 573-58-0
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Appearance Solid
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Molecular Weight 696.66
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Formula C32H22N6Na2O6S2
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Color Pink to red
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SMILES
O=S(C1=C2C=CC=CC2=C(N)C(N=NC3=CC=C(C4=CC=C(N=NC5=CC(S(=O)(O[Na])=O)=C6C=CC=CC6=C5N)C=C4)C=C3)=C1)(O[Na])=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (4)
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Journal Impact Factor
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Most Recent
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Mol Neurodegener
Immunotherapy against tau fragment diminishes AD pathology, improving synaptic function and cognition. [Abstract]2025 May 27;20(1):60. PMID: 40426267 -
Eur J Pharmacol
Fruquintinib/HMPL-013 ameliorates cognitive impairments and pathology in a mouse model of cerebral amyloid angiopathy (CAA). [Abstract]2023 Jan 15:939:175446. PMID: 36470443 -
Cell Oncol (Dordr)
Targeting VCP with V8 suppresses glioblastoma development via formation of aggregates and disruption of mitophagy flux. [Abstract]2026 Jan 8;49(1):21. PMID: 41505038 -
Open Life Sci
2025 Apr 29;20(1):20221062. PMID: 40321158
Solvent & Solubility
DMSO : 20 mg/mL (28.71 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
HeLa cells are selected for these studies due to their large cytoplasmic volume. Cells transfected with mutant or wild-type HSPB1 constructs are grown on coverslips for 24 hr and then are stained with Congo red to determine if the aggregates display amyloidogenic properties. Briefly, cells are first fixed with 10% formalin for 10 min and stained with 1% Congo red for 5 min, followed by destaining with 0.01% potassium hydroxide in 50% ethanol. Coverslips are then passed through graded ethanol concentrations for dehydration and mounted in a mounting medium and examined by fluorescent microscopy under rhodamine filter[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (419 KB)
- English - EN (419 KB)
- Français - FR (419 KB)
- Deutsch - DE (419 KB)
- Norwegian - NO (419 KB)
- Español - ES (419 KB)
- Swedish - SV (419 KB)
- Italian - IT (419 KB)
- Korean - KR (419 KB)
- Portuguese - PT (419 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.4354 mL | 7.1771 mL | 14.3542 mL | 35.8855 mL |
| 5 mM | 0.2871 mL | 1.4354 mL | 2.8708 mL | 7.1771 mL | |
| 10 mM | 0.1435 mL | 0.7177 mL | 1.4354 mL | 3.5886 mL | |
| 15 mM | 0.0957 mL | 0.4785 mL | 0.9569 mL | 2.3924 mL | |
| 20 mM | 0.0718 mL | 0.3589 mL | 0.7177 mL | 1.7943 mL | |
| 25 mM | 0.0574 mL | 0.2871 mL | 0.5742 mL | 1.4354 mL |
Congo Red Related Classifications
HY-D0236 Related Classifications
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