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  3. Indigo carmine

Indigo carmine  (Synonyms: Indigotindisulfonate sodium; C.I.Acid Blue 74)

Cat. No.: HY-W040226 Purity: ≥98.0%
COA Handling Instructions

Indigo carmine is an efficient reagent for the determination of ozone by chemlluminescence (CL).

For research use only. We do not sell to patients.

Indigo carmine Chemical Structure

Indigo carmine Chemical Structure

CAS No. : 860-22-0

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500 mg USD 50 In-stock
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5 g   Get quote  

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This product is a controlled substance and not for sale in your territory.

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Based on 1 publication(s) in Google Scholar

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Description

Indigo carmine is an efficient reagent for the determination of ozone by chemlluminescence (CL)[1].

In Vitro

Indigo carmine in a 0.2% aqueous solution is blue at pH 11.4 and yellow at 13.0. Indigo carmine is also a redox indicator, turning yellow upon reduction[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial
Molecular Weight

466.35

Formula

C16H8N2Na2O8S2

CAS No.
Appearance

Solid

Color

Brown to black

SMILES

O=S(C1=CC2=C(N/C(C2=O)=C3NC4=C(C=C(S(=O)(O[Na])=O)C=C4)C\3=O)C=C1)(O[Na])=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

H2O : 18.52 mg/mL (39.71 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1443 mL 10.7216 mL 21.4431 mL
5 mM 0.4289 mL 2.1443 mL 4.2886 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: ≥98.0%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 2.1443 mL 10.7216 mL 21.4431 mL 53.6078 mL
5 mM 0.4289 mL 2.1443 mL 4.2886 mL 10.7216 mL
10 mM 0.2144 mL 1.0722 mL 2.1443 mL 5.3608 mL
15 mM 0.1430 mL 0.7148 mL 1.4295 mL 3.5739 mL
20 mM 0.1072 mL 0.5361 mL 1.0722 mL 2.6804 mL
25 mM 0.0858 mL 0.4289 mL 0.8577 mL 2.1443 mL
30 mM 0.0715 mL 0.3574 mL 0.7148 mL 1.7869 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Indigo carmine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Indigo carmine
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