Allura Red AC
Based on 1 Customer Validation
Allura Red AC is a food colorant, appearing as a deep red water-soluble powder or granules, used in various applications such as beverages, syrups, candies, and cereals. Allura Red AC can statically quench the intrinsic fluorescence of HSA. Additionally, Allura Red AC is a 5-hydroxytryptamine (5-HT) pathway-associated pro-inflammatory agent, capable of exacerbating experimental colitis. Allura Red AC holds potential for research in inflammatory bowel disease (IBD), intestinal barrier function, and food additive safety.
For research use only. We do not sell to patients.
- Purity: 99.00%
- CAS No.: 25956-17-6
- Formula: C18H14N2Na2O8S2
- Molecular Weight:496.42
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Storage:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Allura Red AC (1 pmol/L, 24 h) promotes 5-HT secretion, upregulates TPH1, and enhances ROS generation in BON cells (EC cell (Enterochromaffin cells) model)[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:BON cells (human enterochromaffin (EC) cell model)
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Concentration:1 pmol/L
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Incubation Time:24 h
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Result:Promoted 5-HT secretion.
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Cell Line:BON cells (human enterochromaffin (EC) cell model)
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Concentration:1 pmol/L
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Incubation Time:24 h
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Result:Upregulated TPH1 mRNA expression.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:DSS (HY-116282)-induced colitis C57BL/6 mouse model[3]
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Dosage:100 ppm (100 mg of Allura Red AC per 1 kg of diet)
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Administration:Dietary intake, 2 weeks (84 days), followed by 3.5% DSS for 7 days
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Result:Exacerbated DSS-induced colitis, reduced body weight, increased disease activity index (DAI), histological scores, and colon weight, shortened colon length, elevated colonic 5-HT, IL-1β, IL-6, and TNF-α levels.
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Animal Model:CD4+CD45RBhi T cell-induced colitis Rag1-/- mouse model[3]
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Dosage:100 ppm (100 mg of Allura Red AC per 1 kg of diet)
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Administration:5 weeks, Dietary intake
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Result:Promoted colitis development, increased body weight loss, disease activity index (DAI), histological scores, and colonic IL-1β, IL-6, TNF-α, and IFN-γ levels.
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Animal Model:Male CD2F1 mice (8 weeks old)[2]
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Dosage:500, 1000, 2000 mg/kg
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Administration:Oral gavage (p.o.), single dose
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Result:Did not show genotoxicity, did not cause DNA damage, and did not induce micronuclei in bone marrow.
Chemical Information
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CAS No. 25956-17-6
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Appearance Solid
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Molecular Weight 496.42
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Formula C18H14N2Na2O8S2
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Color Brown to reddish brown
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SMILES
O=S(C1=CC=C2C(/N=N/C3=CC(C)=C(S(=O)(O[Na])=O)C=C3OC)=C(O)C=CC2=C1)(O[Na])=O
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Synonyms
FD&C RED NO. 40; CI 16035
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
H2O : 100 mg/mL (201.44 mM; Need ultrasonic)
DMSO : 62.5 mg/mL (125.90 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (283 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Di Wu, et al. Characterisation of Interaction Between Food Colourant Allura Red AC and Human Serum Albumin: Multispectroscopic Analyses and Docking Simulations. Food Chem. 2015 Mar 1;170:423-9. [Content Brief]
[2]. Honma M. Evaluation of the in vivo genotoxicity of Allura Red AC (Food Red No. 40). Food Chem Toxicol. 2015 Oct;84:270-5. [Content Brief]
[3]. Kwon YH, et al. Chronic exposure to synthetic food colorant Allura Red AC promotes susceptibility to experimental colitis via intestinal serotonin in mice. Nat Commun. 2022 Dec 20;13(1):7617. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 2.0144 mL | 10.0721 mL | 20.1442 mL | 50.3606 mL |
| 5 mM | 0.4029 mL | 2.0144 mL | 4.0288 mL | 10.0721 mL | |
| 10 mM | 0.2014 mL | 1.0072 mL | 2.0144 mL | 5.0361 mL | |
| 15 mM | 0.1343 mL | 0.6715 mL | 1.3429 mL | 3.3574 mL | |
| 20 mM | 0.1007 mL | 0.5036 mL | 1.0072 mL | 2.5180 mL | |
| 25 mM | 0.0806 mL | 0.4029 mL | 0.8058 mL | 2.0144 mL | |
| 30 mM | 0.0671 mL | 0.3357 mL | 0.6715 mL | 1.6787 mL | |
| 40 mM | 0.0504 mL | 0.2518 mL | 0.5036 mL | 1.2590 mL | |
| 50 mM | 0.0403 mL | 0.2014 mL | 0.4029 mL | 1.0072 mL | |
| 60 mM | 0.0336 mL | 0.1679 mL | 0.3357 mL | 0.8393 mL | |
| 80 mM | 0.0252 mL | 0.1259 mL | 0.2518 mL | 0.6295 mL | |
| 100 mM | 0.0201 mL | 0.1007 mL | 0.2014 mL | 0.5036 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Allura Red AC Related Classifications
HY-123630 Related Classifications
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