Brilliant Blue FCF
Based on 6 publication(s) in Google Scholar
Brilliant Blue FCF is an aromatic hydrocarbon, a synthetic dye produced from petroleum and used as a colorant for food and other substances. The solution has a maximum absorption at 628 nm.
For research use only. We do not sell to patients.
- Purity: 96.70%
- CAS No.: 3844-45-9
- Formula: C37H34N2Na2O9S3
- Molecular Weight:792.85
-
Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Brilliant Blue FCF
More
Customer Validation & Images
-
Cell Proliferation/Viability Assay
-
In Vivo Imaging
-
Bio/Physico-chemical Assay
-
IF
Biological Activity
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Prepare the reserved liquid:
1.1 0.1% Brilliant Blue FCF stock solution was prepared in 85% lactic acid.
1.2 Filter through a 0.22-micron filter membrane.
2. Dye
2.1 The fungal and bacterial cell suspensions were adjusted to approximately 105 and 106 CFU/mL, respectively.
2.2 Bacterial or fungal suspensions of 600 μL were suspended with 100 μL dye solution, respectively.
2.3 The dye was applied to the fungal sample for 5 min.
2.4 The maximum absorption peak was observed at 628 nM by fluorescence microscopy.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 3844-45-9
-
Appearance Solid
-
Molecular Weight 792.85
-
Formula C37H34N2Na2O9S3
-
Color Purple to dark purple
-
SMILES
O=S(C1=CC(C/[N+](CC)=C2C=C/C(C=C/2)=C(C3=CC=C(N(CC)CC4=CC=CC(S(=O)(O[Na])=O)=C4)C=C3)\C5=CC=CC=C5S(=O)(O[Na])=O)=CC=C1)([O-])=O
-
Synonyms
Acid Blue 9; FD&C Blue No. 1; E133
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (6)
-
Journal Impact Factor
-
Most Recent
-
Nat Commun
2025 Jul 22;16(1):6734. PMID: 40695820
Brilliant Blue FCF purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jul 22;16(1):6734. [Abstract]
Brilliant Blue FCF (2.5% wt/vol; mixed into standard food; 48 h at 18°C and 24 h at 25°C) was used in the Drosophila Smurf assay, in which blue coloration outside the digestive tract indicated intestinal barrier disruption.
-
Int J Oral Sci
MSCs-derived apoptotic extracellular vesicles promote muscle regeneration by inducing Pannexin 1 channel-dependent creatine release by myoblasts. [Abstract]2023 Jan 16;15(1):7. PMID: 36646698
Brilliant Blue FCF purchased from MedChemExpress. Usage Cited in: Int J Oral Sci. 2023 Jan 16;15(1):7. [Abstract]
Relative creatine change of C2C12 myoblasts supernatants in fusion medium over time with BB-FCF (Brilliant Blue FCF; 10-100 μmol/L; daily for 1-3 days) treatment. BB-FCF inhibited Pannexin 1 channel function and significantly decreased the relative creatine levels in the supernatants of C2C12 myoblasts cultured in fusion medium on Days 1–3, suggesting that extracellular creatine release during myoblast fusion depends on activated Pannexin 1 channels.
Brilliant Blue FCF purchased from MedChemExpress. Usage Cited in: Int J Oral Sci. 2023 Jan 16;15(1):7. [Abstract]
The representative immunofluorescence images and analysis of fusion index of C2C12 myoblasts in control and Pannexin 1 inhibitor BB-FCF (Brilliant Blue FCF; 10-100 μmol/L; 72 h) treated groups. The fusion index of C2C12 myoblasts was significantly inhibited.
-
-
Plant Cell Environ
Exogenous Inhibitors Enhance Plant Resistance to Rice Planthoppers Through Boosting Phenylpropanoid Pathway Under Elevated CO2. [Abstract]2025 Nov 14. PMID: 41236170 -
-
Brilliant Blue FCF purchased from MedChemExpress. Usage Cited in: chemRxiv. 2026 May 17.
Cytotoxicity of Brilliant Blue FCF (100 µM) in NIH/3T3 fibroblasts and HepG2 hepatocellular carcinoma cells following 72 h exposure. Cell viability was normalized to vehicle control, demonstrating minimal intrinsic toxicity of the selected dyes at screening concentrations.
Solvent & Solubility
H2O : 33.33 mg/mL (42.04 mM; ultrasonic and adjust pH to 11 with NaOH)
H2O : 7 mg/mL (8.83 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (278 KB)
-
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / H2O | 1 mM | 1.2613 mL | 6.3064 mL | 12.6127 mL | 31.5318 mL |
| 5 mM | 0.2523 mL | 1.2613 mL | 2.5225 mL | 6.3064 mL | |
| H2O | 10 mM | 0.1261 mL | 0.6306 mL | 1.2613 mL | 3.1532 mL |
| 15 mM | 0.0841 mL | 0.4204 mL | 0.8408 mL | 2.1021 mL | |
| 20 mM | 0.0631 mL | 0.3153 mL | 0.6306 mL | 1.5766 mL | |
| 25 mM | 0.0505 mL | 0.2523 mL | 0.5045 mL | 1.2613 mL | |
| 30 mM | 0.0420 mL | 0.2102 mL | 0.4204 mL | 1.0511 mL | |
| 40 mM | 0.0315 mL | 0.1577 mL | 0.3153 mL | 0.7883 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Brilliant Blue FCF Related Classifications
HY-D0915 Related Classifications
Powered by Bioz