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  4. Brilliant Blue FCF

Brilliant Blue FCF  (Synonyms: Acid Blue 9; FD&C Blue No. 1; E133)

Cat. No.: HY-D0915 Purity: 96.70%
SDS
COA
Handling Instructions Technical Support

Brilliant Blue FCF is an aromatic hydrocarbon, a synthetic dye produced from petroleum and used as a colorant for food and other substances. The solution has a maximum absorption at 628 nm.

For research use only. We do not sell to patients.

Brilliant Blue FCF Chemical Structure

Brilliant Blue FCF Chemical Structure

CAS No. : 3844-45-9

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in Water In-stock
Solid
5 g In-stock
10 g In-stock
50 g   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 2 publication(s) in Google Scholar

Brilliant Blue FCF Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Brilliant Blue FCF is an aromatic hydrocarbon, a synthetic dye produced from petroleum and used as a colorant for food and other substances. The solution has a maximum absorption at 628 nm.

In Vitro

1. Prepare the reserved liquid:
0.1% Brilliant Blue FCF stock solution was prepared in 85% lactic acid.
0.22μM filter membrane filtration.
2. Dye
The fungal and bacterial cell suspensions were adjusted to approximately 105 and 106 CFU/mL, respectively.
Bacterial or fungal suspensions of 600 μl were suspended with 100 μl dye solution, respectively.
The dye was applied to the fungal sample for 5 min.
The maximum absorption peak was observed at 628 nM by fluorescence microscopy.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Brilliant Blue FCF Related Antibodies
Molecular Weight

792.85

Formula

C37H34N2Na2O9S3
CAS No.
Appearance

Solid

Color

Pale purple to purple

SMILES

O=S(C1=CC(C/[N+](CC)=C2C=C/C(C=C/2)=C(C3=CC=C(N(CC)CC4=CC=CC(S(=O)(O[Na])=O)=C4)C=C3)\C5=CC=CC=C5S(=O)(O[Na])=O)=CC=C1)([O-])=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

H2O : 33.33 mg/mL (42.04 mM; ultrasonic and adjust pH to 11 with NaOH)

H2O : 7 mg/mL (8.83 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2613 mL 6.3064 mL 12.6127 mL
5 mM 0.2523 mL 1.2613 mL 2.5225 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 25 mg/mL (31.53 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: ≥98.0%

SDS (394 KB)

COA (258 KB) HNMR (191 KB) LCMS (304 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / H2O 1 mM 1.2613 mL 6.3064 mL 12.6127 mL 31.5318 mL
5 mM 0.2523 mL 1.2613 mL 2.5225 mL 6.3064 mL
H2O 10 mM 0.1261 mL 0.6306 mL 1.2613 mL 3.1532 mL
15 mM 0.0841 mL 0.4204 mL 0.8408 mL 2.1021 mL
20 mM 0.0631 mL 0.3153 mL 0.6306 mL 1.5766 mL
25 mM 0.0505 mL 0.2523 mL 0.5045 mL 1.2613 mL
30 mM 0.0420 mL 0.2102 mL 0.4204 mL 1.0511 mL
40 mM 0.0315 mL 0.1577 mL 0.3153 mL 0.7883 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Brilliant Blue FCF Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Brilliant Blue FCF3844-45-9Acid Blue 9 FD&C Blue No. 1 E133Acid Blue9Acid Blue-9E133E 133E-133Fluorescent DyeColorantBacterialFungalInhibitorinhibitorinhibit

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