AF 594 azide triethylamine

Cat. No.: HY-D2161A Purity: 95.50%: Data Sheet
COA

SDS
Handling Instructions
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AF 594 azide (triethylamine) is an azide derivative of the red fluorescent dye AF 594, which has high fluorescence quantum yield and high photostability (maximum absorption wavelength of 586 nm, maximum emission wavelength of 613 nm). AF 594 azide (triethylamine) forms stable adducts by reaction of the azide group with alkynyl derivatives (terminal alkynes and cyclooctyne).

For research use only. We do not sell to patients.

AF 594 azide triethylamine Chemical Structure

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Other Forms of AF 594 azide triethylamine:

AF 594 azide Get quote

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Biological Activity
Purity & Documentation
References
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Description

Molecular Weight

906.08

Formula

C₄₄H₅₅N₇O₁₀S₂

Appearance

Solid

Color

Pale purple to purple

SMILES

CN1C(C)(C=C(C2=C1C=C(C3=C2)OC4=CC5=[N+](C(C)(C=C(C5=CC4=C3C6=C(C=CC(C(NCCCN=[N+]=[N-])=O)=C6)C(O)=O)CS(=O)([O-])=O)C)C)CS(=O)([O-])=O)C.CC[NH+](CC)CC

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (110.37 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.1037 mL	5.5183 mL	11.0366 mL
5 mM	0.2207 mL	1.1037 mL	2.2073 mL
10 mM	0.1104 mL	0.5518 mL	1.1037 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

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Working solution concentration: mg/mL

Purity & Documentation

Purity: 95.50%

Select Batch:

Data Sheet (272 KB) SDS (252 KB)

COA (251 KB) HNMR (150 KB) RP-HPLC (265 KB) MS (134 KB)

Handling Instructions (2659 KB)

References

[1]. Whitson K B, et al. Preparation and characterization of Alexa Fluor 594-labeled epidermal growth factor for fluorescence resonance energy transfer studies: application to the epidermal growth factor receptor[J]. Analytical biochemistry, 2004, 324(2): 227-236.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.1037 mL	5.5183 mL	11.0366 mL	27.5914 mL
	5 mM	0.2207 mL	1.1037 mL	2.2073 mL	5.5183 mL
	10 mM	0.1104 mL	0.5518 mL	1.1037 mL	2.7591 mL
	15 mM	0.0736 mL	0.3679 mL	0.7358 mL	1.8394 mL
	20 mM	0.0552 mL	0.2759 mL	0.5518 mL	1.3796 mL
	25 mM	0.0441 mL	0.2207 mL	0.4415 mL	1.1037 mL
	30 mM	0.0368 mL	0.1839 mL	0.3679 mL	0.9197 mL
	40 mM	0.0276 mL	0.1380 mL	0.2759 mL	0.6898 mL
	50 mM	0.0221 mL	0.1104 mL	0.2207 mL	0.5518 mL
	60 mM	0.0184 mL	0.0920 mL	0.1839 mL	0.4599 mL
	80 mM	0.0138 mL	0.0690 mL	0.1380 mL	0.3449 mL
	100 mM	0.0110 mL	0.0552 mL	0.1104 mL	0.2759 mL