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  5. Pueraria lobate (Willd.) Ohwi
  6. Tectorigenin

Tectorigenin is a plant isoflavonoid originally isolated from the dried flower of Pueraria lobate Benth.

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CAS No. : 548-77-6

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무료 샘플 (0.1 - 0.2 mg)   지금 신청하기  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 해외재고보유
Solution
10 mM * 1 mL in DMSO 해외재고보유
Solid
5 mg 해외재고보유
10 mg 해외재고보유
25 mg 해외재고보유
50 mg 해외재고보유
100 mg 해외재고보유
200 mg   견적 받기  
500 mg   견적 받기  

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고객리뷰

Based on 7 publication(s) in Google Scholar

Other Forms of Tectorigenin:

Tectorigenin Related Antibodies

Top Publications Citing Use of Products

    Tectorigenin purchased from MedChemExpress. Usage Cited in: Br J Pharmacol. 2021 Jun;178(12):2443-2460.  [Abstract]

    C57BL/6J mice were treated with ANIT and/or Tectorigenin (TEC). Serum levels of AST, ALT, γ-GT, and AP were measured by ELISA kits, n = 6. The results showed that Tectorigenin (75 mg/kg; oral gavage; once daily for 5 days) significantly alleviated the reduction of liver function markers induced by ANIT in an ANIT-induced mouse model of intrahepatic cholestasis.

    Tectorigenin purchased from MedChemExpress. Usage Cited in: Br J Pharmacol. 2021 Jun;178(12):2443-2460.  [Abstract]

    C57BL/6J mice were treated with ANIT and/or Tectorigenin (TEC). Representative images of hepatic HE staining were shown. The results showed that Tectorigenin (75 mg/kg; oral gavage; once daily for 5 days) significantly ameliorated extensive tissue necrosis induced by ANIT in an ANIT-induced mouse model of intrahepatic cholestasis.

    Tectorigenin purchased from MedChemExpress. Usage Cited in: Br J Pharmacol. 2021 Jun;178(12):2443-2460.  [Abstract]

    C57BL/6J mice were treated with ANIT and/or Tectorigenin (TEC). Representative images of hepatic TUNEL staining (left) and quantitative statistics (right) were shown in the figure, n = 6. The results showed that Tectorigenin (TEC) (75 mg/kg; oral gavage; once daily for 5 days) inhibited ANIT-induced apoptotic cell death, as shown by fewer positive areas after TUNEL staining.

    Tectorigenin purchased from MedChemExpress. Usage Cited in: Br J Pharmacol. 2021 Jun;178(12):2443-2460.  [Abstract]

    C57BL/6J mice were treated with ANIT and/or Tectorigenin (TEC). The hepatic activity of caspase-3 was determined. The results showed that Tectorigenin (TEC) (75 mg/kg; oral gavage; once daily for 5 days) inhibited ANIT-induced apoptotic cell death, as shown by reduced caspase-3 activity.

    Tectorigenin purchased from MedChemExpress. Usage Cited in: Br J Pharmacol. 2021 Jun;178(12):2443-2460.  [Abstract]

    Western blot analysis was performed to determine the protein level of FXR and NR1H3 in mouse liver, and quantitative statistics are shown in the right panel, n = 5. The results showed that Tectorigenin (TEC) (75 mg/kg; oral gavage; once daily for 5 days) treatment restored the ANIT-induced decrease in the protein levels of FXR and NR1H3.

    • Biological Activity

    • Protocol

    • 순도&문서

    • References

    • 고객리뷰

    제품 설명

    Tectorigenin is a plant isoflavonoid originally isolated from the dried flower of Pueraria lobate Benth.

    In Vitro

    Tectorigenin is a plant isoflavonoid originally isolated from the dried flower of Pueraria thomsonii Benth. Palmitic acid (PA)-stimulated ROS production is abolished by treatment with Tectorigenin for HUVECs in a dose-dependent manner (0.1, 1, 10 μM). Treatment with Tectorigenin attenuates enhanced IKKβ phosphorylation and effectively blocks NF-κB activation by inhibition of p65 phosphorylation at concentrations ranging from 0.1 to 10 μM. Tectorigenin treatment also effectively inhibits PA-augmented TNF-α and IL-6 production in a concentration dependent manner[1]. The number of viable HepG2 cells treated by Tectorigenin decreases in a concentration- and time-dependent manner. When HepG2 cells are treated with Tectorigenin at 5, 10 and 20 mg/L for 24 h, the viability rate is 91%, 79% and 62%, respectively[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Tectorigenin Related Antibodies
    분자량

    300.26

    화학식

    C16H12O6
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    O=C1C(C2=CC=C(O)C=C2)=COC3=CC(O)=C(OC)C(O)=C13
    Structure Classification
    Initial Source
    선적

    Room temperature in continental US; may vary elsewhere.
    보관
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    용액&용해도
    In Vitro: 

    DMSO : 100 mg/mL (333.04 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.3304 mL 16.6522 mL 33.3045 mL
    5 mM 0.6661 mL 3.3304 mL 6.6609 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • 몰농도 계산기

    • 농도 희석 계산기

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (6.93 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (6.93 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    순도&문서

    Purity: 99.89%

    SDS (393 KB)

    COA (248 KB) HNMR (251 KB) LCMS (95 KB)

    Handling Instructions (2659 KB)
    References
    Kinase Assay
    [1]

    HUVECs grown to confluence in 24-well plates are pretreated with Tectorigenin (0.1, 1, 10 μM), salicylate (5 mM) or GSH (1 mM) for 30 min, then stimulated with Palmitic acid (PA) (100 μM) for further 12 h in serum-free medium, and the medium is then collected on ice. The levels of TNF-α and IL-6 in the supernatant are assayed with commercial ELISA Kits[1].

    MCE 는 독립적으로 이러한 방법들의 정확성을 확인하지 않았습니다. 참고용으로만 봐주십시오.
    Cell Assay 
    [2]

    Cell viability is assessed by MTT method. Briefly, cells are seeded in 96-well plate at a density of 1×104 cells/well. After 24 h incubation, Tectorigenin at different concentrations is added to the cells while only DMSO (solvent) is added as a negative control. After growing for 12, 24 and 48 h, cells are incubated with MTT (0.5 mg/mL) for 4 h at 37°C. During this incubation period, water-insoluble formazan crystals are formed, which are dissolved by the addition of 100 μL/well DMSO. The optical densities at 570 nm are measured using an enzyme-linked immunosorbent assay plate reader. Wells containing culture medium and MTT but no cells act as blanks[2].

    MCE 는 독립적으로 이러한 방법들의 정확성을 확인하지 않았습니다. 참고용으로만 봐주십시오.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.3304 mL 16.6522 mL 33.3045 mL 83.2612 mL
    5 mM 0.6661 mL 3.3304 mL 6.6609 mL 16.6522 mL
    10 mM 0.3330 mL 1.6652 mL 3.3304 mL 8.3261 mL
    15 mM 0.2220 mL 1.1101 mL 2.2203 mL 5.5507 mL
    20 mM 0.1665 mL 0.8326 mL 1.6652 mL 4.1631 mL
    25 mM 0.1332 mL 0.6661 mL 1.3322 mL 3.3304 mL
    30 mM 0.1110 mL 0.5551 mL 1.1101 mL 2.7754 mL
    40 mM 0.0833 mL 0.4163 mL 0.8326 mL 2.0815 mL
    50 mM 0.0666 mL 0.3330 mL 0.6661 mL 1.6652 mL
    60 mM 0.0555 mL 0.2775 mL 0.5551 mL 1.3877 mL
    80 mM 0.0416 mL 0.2082 mL 0.4163 mL 1.0408 mL
    100 mM 0.0333 mL 0.1665 mL 0.3330 mL 0.8326 mL
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    Tectorigenin Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Tectorigenin548-77-6OthersInhibitorinhibitorinhibit

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    상품명:
    Tectorigenin
    Cat. No.:
    HY-N0792
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