BI 99179

Name: BI 99179
Brand: MedChemExpress
SKU: HY-16100
Rating: 5.0 (3 reviews)

Cat. No.: HY-16100 Pureza: 99.38%: Ficha de datos
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BI 99179, a chemical probe, is a potent and selective type I fatty acid synthase (FAS) inhibitor with an IC₅₀ of 79 nM. BI 99179 is a tool compound suitable for the in vivo validation of FAS as a target for lipid metabolism related diseases. BI 99179 exhibits significant exposure (both peripheral and central) upon oral administration in rats.

Para uso exclusivo en investigación. No vendemos a pacientes.

The BI 99179 was designed by Boehringer Ingelheim and could be obtained free of charge through the Boehringer Ingelheim open innovation portal opnMe.com, associated with its negative control.

BI 99179 Estructura química

No. CAS : 1291779-76-4

Tamaño	Stock	Cantidad

Muestra gratis (0.1 - 0.2 mg)	Solicite ahora
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	En stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	En stock	Estimated Time of Arrival: December 31
Solid
5 mg	En stock	Estimated Time of Arrival: December 31
10 mg	En stock	Estimated Time of Arrival: December 31
25 mg	En stock	Estimated Time of Arrival: December 31
50 mg	En stock	Estimated Time of Arrival: December 31
100 mg	En stock	Estimated Time of Arrival: December 31
200 mg	Obtener un presupuesto
500 mg	Obtener un presupuesto

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Revisión del cliente

Based on 3 publication(s) in Google Scholar

3 Publications Citing Use of MCE BI 99179

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Descripciòn

IC₅₀ & Target

IC50: 79 nM (FASN)^[1]

In Vitro

BI 99179 is potent in the mouse hypothalamic N-42 cell with an IC₅₀ of 0.6 μM. BI 99179 shows no significant LDH release in the cytotoxicity assay up to 30 μM^[1].
BI 99179 (BI-99179; 1, 2, and 4 μM) shows antiproliferative efficacy in human glioma GAMG cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[2]

Cell Line:	Human glioma GAMG cells
Concentration:	1, 2, 4 µM
Incubation Time:	96 to 120 hours
Result:	The optimal palmitate concentration for GAMG cell line was 4 µM.

In Vivo

BI 99179 has a super pharmacokinetic profile in male Han/Wistar rats (oral application of 4 mg/kg) with half life (t_1/2) of 3.0 h^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Peso molecular

391.46

Fòrmula

C₂₃H₂₅N₃O₃

No. CAS

1291779-76-4

Appearance

Solid

Color

White to off-white

SMILES

O=C([C@H]1C[C@@H](NC(CC)=O)CC1)N(C2=CC=C(C3=NC4=CC=CC=C4O3)C=C2)C

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvente y solubilidad

In Vitro:

DMSO : 33.33 mg/mL (85.14 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.5545 mL	12.7726 mL	25.5452 mL
5 mM	0.5109 mL	2.5545 mL	5.1090 mL
10 mM	0.2555 mL	1.2773 mL	2.5545 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculadora de molaridad
Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.31 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (5.31 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (5.31 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Pureza y Documentación

Purity: 99.38%

Select Batch:

Ficha de datos (273 KB) SDS (394 KB)

COA (246 KB) LCMS (95 KB)

Instrucciones de manejo (2659 KB)

Referencias

[1]. Kley JT, et al. Discovery of BI 99179, a potent and selective inhibitor of type I fatty acid synthase with central exposure.Bioorg Med Chem Lett. 2011 Oct 1;21(19):5924-7. [Content Brief]

[2]. Prosanta K Singha, et al. Evaluation of FASN inhibitors by a versatile toolkit reveals differences in pharmacology between human and rodent FASN preparations and in antiproliferative efficacy in vitro vs. in situ in human cancer cells. Eur J Pharm Sci. 2020 Apr 7;149:105321. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.5545 mL	12.7726 mL	25.5452 mL	63.8630 mL
	5 mM	0.5109 mL	2.5545 mL	5.1090 mL	12.7726 mL
	10 mM	0.2555 mL	1.2773 mL	2.5545 mL	6.3863 mL
	15 mM	0.1703 mL	0.8515 mL	1.7030 mL	4.2575 mL
	20 mM	0.1277 mL	0.6386 mL	1.2773 mL	3.1932 mL
	25 mM	0.1022 mL	0.5109 mL	1.0218 mL	2.5545 mL
	30 mM	0.0852 mL	0.4258 mL	0.8515 mL	2.1288 mL
	40 mM	0.0639 mL	0.3193 mL	0.6386 mL	1.5966 mL
	50 mM	0.0511 mL	0.2555 mL	0.5109 mL	1.2773 mL
	60 mM	0.0426 mL	0.2129 mL	0.4258 mL	1.0644 mL
	80 mM	0.0319 mL	0.1597 mL	0.3193 mL	0.7983 mL

BI 99179 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BI 991791291779-76-4BI99179BI-99179Fatty Acid Synthase (FASN)FattyacidsynthaseFASCNSexposureFASNInhibitorinhibitorinhibit

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BI 99179

Revisión del cliente

BI 99179 Related Antibodies

3 Publications Citing Use of MCE BI 99179

Recomendaciones destacadas

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Actividad biológica

Pureza y Documentación

Referencias

Revisión del cliente

BI 99179 Related Antibodies

Calculadora de molaridad

Calculadora de dilución

Complete Stock Solution Preparation Table

BI 99179 Related Classifications

Keywords:

Productos vistos recientemente:

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