2. Stem Cell/Wnt
  3. Wnt
  4. Coronaridine

Coronaridine, an iboga type alkaloid, inhibits the wnt signaling pathway by decreasing β-catenin expression.

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Coronaridine

Coronaridine 構造式

CAS 番号 : 467-77-6

容量 価格（税別） 在庫状況 数量
1 mg $650 在庫あり
5 mg $1655 在庫あり
10 mg $2650 在庫あり
50 mg   お問い合わせ  
100 mg   お問い合わせ  

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Based on 1 publication(s) in Google Scholar

Coronaridine 関連抗体

Top Publications Citing Use of Products

Wnt アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
Wnt1 Wnt3 Wnt5 Wnt7 Wnt11

  • 生物活性

  • 純度とドキュメンテーション

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製品説明

Coronaridine, an iboga type alkaloid, inhibits the wnt signaling pathway by decreasing β-catenin expression[1].

Cellular Effect
Cell Line Type Value Description References
A-431 ED50
19.1 6
Compound: 1
Cytotoxicity against human A431 cells by SRB assay
Cytotoxicity against human A431 cells by SRB assay
[PMID: 7853002]
BC1 cell line ED50
7.5 6
Compound: 1
Cytotoxicity against human BC1 cells by SRB assay
Cytotoxicity against human BC1 cells by SRB assay
[PMID: 7853002]
Col2 ED50
> 20 6
Compound: 1
Cytotoxicity against human Col2 cells by SRB assay
Cytotoxicity against human Col2 cells by SRB assay
[PMID: 7853002]
DLD-1 IC50
24.4 3
Compound: 3
Cytotoxicity against Wnt-dependent human DLD1 cells assessed as cell viability after 24 hrs by fluorescence assay
Cytotoxicity against Wnt-dependent human DLD1 cells assessed as cell viability after 24 hrs by fluorescence assay
[PMID: 26231157]
HCT-116 IC50
11.6 3
Compound: 3
Cytotoxicity against Wnt-dependent human HCT116 cells assessed as cell viability after 24 hrs by fluorescence assay
Cytotoxicity against Wnt-dependent human HCT116 cells assessed as cell viability after 24 hrs by fluorescence assay
[PMID: 26231157]
HEK293 IC50
31.7 3
Compound: 3
Cytotoxicity against HEK293 cells assessed as cell viability after 24 hrs by fluorescence assay
Cytotoxicity against HEK293 cells assessed as cell viability after 24 hrs by fluorescence assay
[PMID: 26231157]
HEK-293T IC50
> 40 3
Compound: 3
Cytotoxicity against HEK293T cells assessed as cell viability after 24 hrs by fluorescence assay
Cytotoxicity against HEK293T cells assessed as cell viability after 24 hrs by fluorescence assay
[PMID: 26231157]
HeLa EC50
> 40 3
Compound: 20
Inhibition of lysosomal acidification in human HeLa cells incubated for 3 hrs by LysoTracker Red DND-99 staining based array scan method
Inhibition of lysosomal acidification in human HeLa cells incubated for 3 hrs by LysoTracker Red DND-99 staining based array scan method
[PMID: 32356659]
HT ED50
> 20 6
Compound: 1
Cytotoxicity against human HT cells by SRB assay
Cytotoxicity against human HT cells by SRB assay
[PMID: 7853002]
KB ED50
13.6 6
Compound: 1
Cytotoxicity against human KB cells by SRB assay
Cytotoxicity against human KB cells by SRB assay
[PMID: 7853002]
KB-V1 ED50
1.9 6
Compound: 1
Cytotoxicity against human drug-resistant KBV1 cells by SRB assay in presence of vinblastine
Cytotoxicity against human drug-resistant KBV1 cells by SRB assay in presence of vinblastine
[PMID: 7853002]
KB-V1 ED50
11.2 6
Compound: 1
Cytotoxicity against human drug-resistant KBV1 cells by SRB assay in absence of vinblastine
Cytotoxicity against human drug-resistant KBV1 cells by SRB assay in absence of vinblastine
[PMID: 7853002]
LNCaP ED50
10.7 6
Compound: 1
Cytotoxicity against human LNCAP cells by SRB assay
Cytotoxicity against human LNCAP cells by SRB assay
[PMID: 7853002]
Lu1 ED50
10.9 6
Compound: 1
Cytotoxicity against human Lu1 cells by SRB assay
Cytotoxicity against human Lu1 cells by SRB assay
[PMID: 7853002]
P388 ED50
3.8 6
Compound: 1
Cytotoxicity against mouse P388 cells after 48 hrs by SRB assay
Cytotoxicity against mouse P388 cells after 48 hrs by SRB assay
[PMID: 7853002]
RAW264.7 IC50
25.5 3
Compound: 10
Anti-inflammatory activity in mouse RAW264.7 cells assessed as LPS-stimulated inhibition of nitric oxide production incubated simultaneously with LPS for 24 hrs by Griess reagent based assay
Anti-inflammatory activity in mouse RAW264.7 cells assessed as LPS-stimulated inhibition of nitric oxide production incubated simultaneously with LPS for 24 hrs by Griess reagent based assay
[PMID: 26024020]
RKO IC50
> 40 3
Compound: 3
Cytotoxicity against Wnt-independent human RKO cells assessed as cell viability after 24 hrs by fluorescence assay
Cytotoxicity against Wnt-independent human RKO cells assessed as cell viability after 24 hrs by fluorescence assay
[PMID: 26231157]
SK-MEL-2 ED50
> 20 6
Compound: 1
Cytotoxicity against human SK-MEL-2 cells by SRB assay
Cytotoxicity against human SK-MEL-2 cells by SRB assay
[PMID: 7853002]
SW480 IC50
10.4 3
Compound: 3
Cytotoxicity against Wnt-dependent human SW480 cells assessed as cell viability after 24 hrs by fluorescence assay
Cytotoxicity against Wnt-dependent human SW480 cells assessed as cell viability after 24 hrs by fluorescence assay
[PMID: 26231157]
U-373MG ATCC ED50
12 6
Compound: 1
Cytotoxicity against human U373 cells by SRB assay
Cytotoxicity against human U373 cells by SRB assay
[PMID: 7853002]
ZR-75-1 ED50
> 20 6
Compound: 1
Cytotoxicity against human ZR-75-1 cells by SRB assay
Cytotoxicity against human ZR-75-1 cells by SRB assay
[PMID: 7853002]
体外実験

Coronaridine (0-40 μM; 24 hours) is against non-cancer cells with IC50 values >40 μM. It agaisnt wnt-dependent cells with IC50 values of 10.4, 11.6 and 24.4 μM for SW480, HCT116 and DLD1cells, respectively[1].
Coronaridine (0-40 μM; 24 hours) inhibits β-catenin expression, but the protein levels of p-β--catenin at Ser33, Ser37, and Thr41 and p-β-catenin at Ser 45 [p-b-catenin (S45)] are unchanged[1].
In whole-cell patch clamp recordings,Catharanthine (1-300 μM) are respectively co-applied with GABA at concentrations corresponding to the EC30 value for each receptor subtype. Both congeners potentiated different GABAARs in a concentration-dependent manner[2].
At higher concentrations, however, Catharanthine starts to inhibit GABA-activated currents due to the reduced amplitude and rebound current, where the threshold concentration depended on the receptor subtype (e.g., > 30 μM for hα1β2; > 100 μM for hα1β2γ2 and hα2β2γ2). The PAM activity of Catharanthine's are depended on the receptor subtype: hα1β2 (4.6±0.8 μM), >hα2β2γ2 (12.6±3.8 μM), hα1β2γ2 (14.4 ± 4.6 μM)[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Coronaridine 関連抗体
分子量

338.44

分子式

C21H26N2O2
CAS 番号
Appearance

Solid

Color

White to off-white

SMILES

COC([C@]12[C@@]3([H])[N@@](CCC4=C2NC5=CC=CC=C45)C[C@@](C[C@@H]3CC)([H])C1)=O
Structure Classification
Initial Source
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
溶剤 & 溶解度
体外: 

DMSO : 50 mg/mL (147.74 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9547 mL 14.7737 mL 29.5473 mL
5 mM 0.5909 mL 2.9547 mL 5.9095 mL
10 mM 0.2955 mL 1.4774 mL 2.9547 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 1.25 mg/mL (3.69 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション

純度: 99.72%

COA (254 KB) HNMR (156 KB) LCMS (74 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.9547 mL 14.7737 mL 29.5473 mL 73.8683 mL
5 mM 0.5909 mL 2.9547 mL 5.9095 mL 14.7737 mL
10 mM 0.2955 mL 1.4774 mL 2.9547 mL 7.3868 mL
15 mM 0.1970 mL 0.9849 mL 1.9698 mL 4.9246 mL
20 mM 0.1477 mL 0.7387 mL 1.4774 mL 3.6934 mL
25 mM 0.1182 mL 0.5909 mL 1.1819 mL 2.9547 mL
30 mM 0.0985 mL 0.4925 mL 0.9849 mL 2.4623 mL
40 mM 0.0739 mL 0.3693 mL 0.7387 mL 1.8467 mL
50 mM 0.0591 mL 0.2955 mL 0.5909 mL 1.4774 mL
60 mM 0.0492 mL 0.2462 mL 0.4925 mL 1.2311 mL
80 mM 0.0369 mL 0.1847 mL 0.3693 mL 0.9234 mL
100 mM 0.0295 mL 0.1477 mL 0.2955 mL 0.7387 mL
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Coronaridine Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Coronaridine467-77-6WntNon-cancercancersedativeInhibitorinhibitorinhibit

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製品名:
Coronaridine
製品番号:
HY-121118
数量:
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