Deoxynivalenol (Synonyms: Vomitoxin; デオキシニバレノール溶液)

Name: Deoxynivalenol
Brand: MedChemExpress
SKU: HY-N6684
Rating: 5.0 (16 reviews)

製品番号: HY-N6684 純度: 99.90%: Data Sheet SDS COA 取扱説明書
FAQs
Technical Support

Deoxynivalenol, an orally active mycotoxin of the trichothecenes family, crosses the intestinal mucosa by a paracellular pathway through the tight junctions. The Deoxynivalenol transport is not affected by P-glycoprotein (PgP) or multidrug resistance-associated proteins (MRPs) inhibitors.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号 : 51481-10-8

容量	価格（税別）	在庫状況	数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 440	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 440	在庫あり	Estimated Time of Arrival: December 31
Solid
1 mg	$110	在庫あり	Estimated Time of Arrival: December 31
5 mg	$400	在庫あり	Estimated Time of Arrival: December 31
10 mg	$700	在庫あり	Estimated Time of Arrival: December 31
50 mg		お問い合わせ
100 mg		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 16 publication(s) in Google Scholar

Other Forms of Deoxynivalenol:

Deoxynivalenol-¹³C₁₅ 在庫あり
Deoxynivalenol (Standard) お問い合わせ

顧客検証

Cell Proliferation/Viability Assay

Histological Imaging/Staining

RT-PCR

: Deoxynivalenol purchased from MedChemExpress. Usage Cited in: Environ Int. 2025 Oct 28:205:109891. [Abstract]; Deoxynivalenol (DON, 0.5-4 μM; 24 h) effects on cell viability of IPEC-J2 cells.

: Deoxynivalenol purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2024 Nov 1:286:117243. [Abstract]; Histological characterizations in the kidney sections were evaluated. The results showed that compared with the control group, Deoxynivalenol (DON, 2.5-5 mg/kg; i.p.; single dose) treatment increased inflammatory cell infiltration, hemorrhage, tubular degeneration, and glomerular atrophy in the kidneys of mice. (magnification:×400, scale bar: 50 μm). G: glomerulus, P: proximal tubule, D: distal tubule; Hemorrhage (Δ), inflammatory cell infiltration (↓), tubular degradation (⇒), glomerular atrophy (▲).

: Deoxynivalenol purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2024 Nov 1:286:117243. [Abstract]; Deoxynivalenol (DON, 2.5–5 mg/kg; i.p., single dose) significantly upregulated the mRNA expression levels of the proinflammatory cytokines IL-6, IL-8, IL-1β, and TNF-α in mouse renal tissues.

: Deoxynivalenol purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2024 Nov 1:286:117243. [Abstract]; Protein expression levels of Caspase12, Cleaved-caspase3, Bax, and Bcl-2 were assessed in the kidneys of mice treated with Deoxynivalenol (DON, i.p.; single dose) at either 2.5 or 5 mg/kg BW for 3 hours.

: Deoxynivalenol purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2022 Dec 15:248:114276. [Abstract]; Deoxynivalenol (DON) exposure results in the decrease of the expression levels of occludin and claudin-1 in duodenum of mice.

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製品説明

Cellular Effect

Cell Line	Type	Value	Description	References
COLO 201	IC₅₀	86 3 Compound: 2; DON	Antiproliferative activity against human COLO201 cells after 24 hrs by WST-1 assay Antiproliferative activity against human COLO201 cells after 24 hrs by WST-1 assay	[PMID: 30797671]
COLO 201	IC₅₀	86 3 Compound: 2; DON	Antiproliferative activity against human COLO201 cells after 24 hrs by WST-1 assay Antiproliferative activity against human COLO201 cells after 24 hrs by WST-1 assay	[PMID: 30797671]
COLO 201	IC₅₀	86 3 Compound: 2; DON	Antiproliferative activity against human COLO201 cells after 24 hrs by WST-1 assay Antiproliferative activity against human COLO201 cells after 24 hrs by WST-1 assay	[PMID: 30797671]
K562	EC₅₀	1010 1 Compound: DON	Cytotoxicity against human K562 cells by MTT assay Cytotoxicity against human K562 cells by MTT assay	[PMID: 18327910]
K562	EC₅₀	1010 1 Compound: DON	Cytotoxicity against human K562 cells by MTT assay Cytotoxicity against human K562 cells by MTT assay	[PMID: 18327910]
K562	EC₅₀	1010 1 Compound: DON	Cytotoxicity against human K562 cells by MTT assay Cytotoxicity against human K562 cells by MTT assay	[PMID: 18327910]

体外実験

Deoxynivalenol (0-2 μg/mL, 24 h) provokes the phosphorylation of the mitogen-activated protein kinases (MAPKs) Erk1/2, p38 and SAPK/JNK in Caco-2 cells^[1].
Deoxynivalenol (100-4000 ng/mL, 24-72 h) reduces the viability of IPEC-1 and IPEC-J2 cells after 48 h and 72 h incubation at the doses of 500-4000 ng/mL^[2].
Deoxynivalenol (0-4000 ng/mL, 24-72 h) increases BrdU incorporation at 200 ng/mL and decreases BrdU incorporation at 2000-4000 ng/mL in IPEC-1 and IPEC-J2 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:	IPEC-1 and IPEC-J2 cells
Concentration:	100-4000 ng/mL
Incubation Time:	24-72 h
Result:	Reduced the cell count in a time and dose dependent. Increased LDH signals in IPEC-1 after 48 h and 72 h incubation with 2000 ng/mL and in IPEC-J2 after 48 h incubation with 2000 ng/mL. Reduced NR uptake in IPEC-1 and IPEC-J2 cells at the doses of 2000 ng/mL. Decreased the MTT signal in IPEC-1 to 60% and 40% of the control after 48 h and 72 h at the doses of 2000 ng/mL. Decreased the MTT signal in IPEC-J2 to 70% and 58% of the control after 48 h and 72 h at the doses of 2000 ng/mL.

Cell Proliferation Assay^[1]

Cell Line:	Caco-2 cells
Concentration:	0-10 μg/mL
Incubation Time:	48 h
Result:	Inhibited the proliferation of cells in a dose-dependent manner.

Cell Cycle Analysis^[2]

Cell Line:	IPEC-1 and IPEC-J2 cells
Concentration:	200-2000 ng/mL
Incubation Time:	24-72 h
Result:	Increased percentages of pre-G1, S and G2/M phases and decreased of the G0/G1 phase in response to 2000 ng/mL after 48 h in IPEC-1 cells. Decreased the G0/G1 phase in IPEC-J2 cell at the doses of 2000 ng/mL after 48 h. Increased the percentage of pre-G1 in IPEC-1 cells after 72 h. Increased the G2/M phase in IPEC-1 and IPEC-J2 cells at the doses of 2000 ng/mL after 72 h.

Apoptosis Analysis^[2]

Cell Line:	IPEC-1 and IPEC-J2 cells
Concentration:	200-2000 ng/mL
Incubation Time:	6-72 h
Result:	Increased the caspase 3 activity after 6 h, 8 h, 48 h and 72 h of incubation with 2000 ng/mL. Reduced the caspase 3 activity to the control level after 24 h of incubation with 2000 ng/mL.

Western Blot Analysis^[1]

Cell Line:	Caco-2 cells
Concentration:	2 μg/mL
Incubation Time:	5-240 min
Result:	Increased the phosphorylation of Erk1/Erk2 during 5 min. Increased the phosphorylation of p38 from 5 to 30 min.

Western Blot Analysis^[2]

Cell Line:	IPEC-1 and IPEC-J2 cells
Concentration:	200-2000 ng/mL
Incubation Time:	48 h
Result:	Reduced the protein level of ZO-1 at the doses of 2000 ng/mL.

体内実験

Deoxynivalenol (0-5 mg/kg, i.g., daily, 14 days) increases the number of pregnant rats with excessive salivation in a dose-related manner^[3].
Deoxynivalenol (0-5 mg/kg, i.g., daily, 14 days) causes abnormal sternum development in fetuses rats at a dose of 5 mg/kg^[3].
Deoxynivalenol (10-1000 mg/kg; p.o., i.p.; once time) is estimated to be 78 mg/kg (p.o.) and 49 mg/kg (i.p.) of LD50 values in the B6C3F1 mouse^[4].
Deoxynivalenol (2-20 mg/kg, p.o., daily, 4 weeks) increases in plasma in a dose-dependent manner and reduces weight gain in B6C3F1 mouse^[5].
1.19
Pharmacokinetic Analysis in B6C3F1 Mice Model^[5]

Route	Dose (mg/kg)	Plasma T_1/2α (min)	Plasma T_1/2β (h)	Liver T_1/2α (min)	Liver T_1/2β (h)	Kidney T_1/2α (min)	Kidney T_1/2β (h)	Spleen T_1/2α (min)	Spleen T_1/2β (h)	Heart T_1/2α (min)	Heart T_1/2β (h)
p.o.	25	20.4	11.8	22	19	47	20.9	29	9	41	12.3

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Pregnant rats^[3]
Dosage:	0-5 mg/kg
Administration:	i.g., daily, 14 days
Result:	Reduced feed consumption and mean body weight gain in the 5 mg/kg group . Reduced feed consumption in the 2.5 mg/kg group during 8-14 days. Decreased the number of viable fetuses at 5 mg/kg. Reduced fetal body weight and crown-rump length at 2.5 and 5 mg/kg. Increased the incidence of runts and the average number of fetuses per litter with internal anomalies at 2.5 and 5 mg/kg. Increased the incidence of misaligned and fused sternebrae at 5 mg/kg. Increased the ratios of liver-to-body-weight and kidneys-to-body-weight.

分子量

296.32

分子式

C₁₅H₂₀O₆

CAS 番号

51481-10-8

Appearance

Solid

Color

White to off-white

SMILES

O=C(C(C)=C1)[C@@H](O)[C@@]2(CO)[C@]1([H])O[C@]3([H])[C@H](O)C[C@@]2(C)[C@@]34OC4

Structure Classification

Initial Source

Microorganisms

Fusarium specie

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

溶剤 & 溶解度

体外:

DMSO : 100 mg/mL (337.47 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 30 mg/mL (101.24 mM; Need ultrasonic and warming)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.3747 mL	16.8736 mL	33.7473 mL
5 mM	0.6749 mL	3.3747 mL	6.7495 mL
10 mM	0.3375 mL	1.6874 mL	3.3747 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.44 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.44 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

純度とドキュメンテーション

純度: 99.90%

Select Batch:

データシート (293 KB) SDS (480 KB)

COA (260 KB) HNMR (280 KB) RP-HPLC (259 KB) MS (229 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Sergent T, et al. Deoxynivalenol transport across human intestinal Caco-2 cells and its effects on cellularmetabolism at realistic intestinal concentrations. Toxicol Lett. 2006 Jul 1;164(2):167-76. [Content Brief]

[2]. Diesing AK, et al. Mycotoxin deoxynivalenol (DON) mediates biphasic cellular response in intestinal porcine epithelial cell lines IPEC-1 and IPEC-J2. Toxicol Lett. 2011 Jan 15;200(1-2):8-18. [Content Brief]

[3]. Collins TF, et al. Effects of deoxynivalenol (DON, vomitoxin) on in utero development in rats. Food Chem Toxicol. 2006 Jun;44(6):747-57. [Content Brief]

[4]. Forsell JH, et al. Comparison of acute toxicities of deoxynivalenol (vomitoxin) and 15-acetyldeoxynivalenol in the B6C3F1 mouse. Food Chem Toxicol. 1987 Feb;25(2):155-62. [Content Brief]

[5]. Pestka JJ, et al. Immunochemical assessment of deoxynivalenol tissue distribution following oral exposure in the mouse. Toxicol Lett. 2008 May 5;178(2):83-7. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol / DMSO	1 mM	3.3747 mL	16.8736 mL	33.7473 mL	84.3682 mL
	5 mM	0.6749 mL	3.3747 mL	6.7495 mL	16.8736 mL
	10 mM	0.3375 mL	1.6874 mL	3.3747 mL	8.4368 mL
	15 mM	0.2250 mL	1.1249 mL	2.2498 mL	5.6245 mL
	20 mM	0.1687 mL	0.8437 mL	1.6874 mL	4.2184 mL
	25 mM	0.1350 mL	0.6749 mL	1.3499 mL	3.3747 mL
	30 mM	0.1125 mL	0.5625 mL	1.1249 mL	2.8123 mL
	40 mM	0.0844 mL	0.4218 mL	0.8437 mL	2.1092 mL
	50 mM	0.0675 mL	0.3375 mL	0.6749 mL	1.6874 mL
	60 mM	0.0562 mL	0.2812 mL	0.5625 mL	1.4061 mL
	80 mM	0.0422 mL	0.2109 mL	0.4218 mL	1.0546 mL
	100 mM	0.0337 mL	0.1687 mL	0.3375 mL	0.8437 mL

Deoxynivalenol Related Classifications

Metabolic Disease

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量		濃度		体積		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）	×	体積 _（開始）	=	濃度 _（終了）	×	体積 _（終了）
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Deoxynivalenol (Synonyms: Vomitoxin; デオキシニバレノール 溶液)

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Other Forms of Deoxynivalenol:

Deoxynivalenol 関連抗体

顧客検証

おすすめ

•関連スクリーニングライブラリ:

•関連小分子:

•関連タンパク質:

生物活性

純度とドキュメンテーション

参考文献

カスタマーレビュー

Deoxynivalenol 関連抗体

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Deoxynivalenol Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

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Deoxynivalenol (Synonyms: Vomitoxin; デオキシニバレノール溶液)