1. Membrane Transporter/Ion Channel
  2. EAAT2
  3. DL-TBOA

DL-TBOA 

Cat. No.: HY-107522 Purity: 99.68%
Handling Instructions

DL-TBOA is a potent non-transportable inhibitor of excitatory amino acid transporters with IC50s of 70 μM, 6 μM and 6 μM for excitatory amino acid transporter-1 (EAAT1), EAAT2 and EAAT3, respectively. DL-TBOA inhibits the uptake of [14C]glutamate in COS-1 cells expressing the human EAAT1 and EAAT2 with Ki valuesof 42 μM and 5.7 μM, respectively. DL-TBOA blocks EAAT4 and EAAT5 in a competitive manner with Ki values of 4.4 μM and 3.2 μM, respectively.

For research use only. We do not sell to patients.

DL-TBOA Chemical Structure

DL-TBOA Chemical Structure

CAS No. : 205309-81-5

Size Price Stock Quantity
Solution
10 mM * 1 mL in DMSO USD 447 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 447 In-stock
Estimated Time of Arrival: December 31
Solid
5 mg USD 500 In-stock
Estimated Time of Arrival: December 31
10 mg USD 860 In-stock
Estimated Time of Arrival: December 31
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100 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

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Description

DL-TBOA is a potent non-transportable inhibitor of excitatory amino acid transporters with IC50s of 70 μM, 6 μM and 6 μM for excitatory amino acid transporter-1 (EAAT1), EAAT2 and EAAT3, respectively. DL-TBOA inhibits the uptake of [14C]glutamate in COS-1 cells expressing the human EAAT1 and EAAT2 with Ki valuesof 42 μM and 5.7 μM, respectively. DL-TBOA blocks EAAT4 and EAAT5 in a competitive manner with Ki values of 4.4 μM and 3.2 μM, respectively[1][2][3].

IC50 & Target

IC50: 70 μM (EAAT1), 6 μM (EAAT2) and 6 μM (EAAT3); Ki: 42 μM (human EAAT1), 5.7 μM (human EAAT2), 4.4 μM (EAAT4) and 3.2 μM (EAAT5)[1][2][3]

In Vitro

DL-TBOA (70-350 μM; 48 hours; HCT116 and LoVo cell lines) treatment concentration-dependently enhances SN38-induced loss of viability. DL-TBOA reversed Oxaliplatin-induced loss of viability[4].
DL-TBOA (350 μM; 24 hours; HCT116 and LoVo cell lines) treatment decreases p53 induction by SN38 and oxaliplatin[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[4]

Cell Line: HCT116 and LoVo cell lines
Concentration: 70 μM, 350 μM
Incubation Time: 48 hours
Result: Enhanced SN38-induced, and counteracted Oxaliplatin-induced, cell death.

Cell Viability Assay[4]

Cell Line: HCT116 and LoVo cell lines
Concentration: 350 μM
Incubation Time: 24 hours
Result: p53 induction by SN38 and oxaliplatin was decreased.
In Vivo

DL-TBOA (10 nmol; i.c.v.) to morphine-dependent rats significantly facilitates the expression of naloxone-precipitated somatic signs and conditioned place aversion[5].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Sprague-Dawley rats (180-250 g)[5]
Dosage: 1 nmol, 3 nmol, 10 nmol
Administration: Intracerebroventricularly injection (i.c.v.)
Result: Dose dependently facilitated various somatic signs induced by Naloxone (0.1 mg/kg)-precipitated morphine withdrawal.
Molecular Weight

239.22

Formula

C₁₁H₁₃NO₅

CAS No.
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 200 mg/mL (836.05 mM; Need ultrasonic)

H2O : 5 mg/mL (20.90 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.1803 mL 20.9013 mL 41.8025 mL
5 mM 0.8361 mL 4.1803 mL 8.3605 mL
10 mM 0.4180 mL 2.0901 mL 4.1803 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 5 mg/mL (20.90 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 5 mg/mL (20.90 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 5 mg/mL (20.90 mM); Clear solution

*All of the co-solvents are provided by MCE.
References

Purity: 99.68%

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DL-TBOA
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