EAAT5

EAAT5 is a retina-enriched excitatory amino acid transporter that binds glutamate and couples transporter activity to a glutamate-gated Cl^- conductance^[1]. Mechanistically, EAAT5 links synaptic glutamate handling with inhibitory anion current, so glutamate released from photoreceptors and rod bipolar cells can activate local feedback within retinal circuits^[2]. In mouse retina, EAAT5 localizes close to glutamatergic release sites, and EAAT5^-/^- retinae show reduced flicker resolution in multielectrode-array recordings, supporting its role in temporal visual information processing^[3]. Compared with related isoforms, EAAT5 differs from high-capacity EAAT1/EAAT2 transporters because high chloride conductance is associated with less effective glutamate removal, whereas EAAT1 and EAAT2 remain major glutamate transporters in retina^[2]^[3]. At rod bipolar terminals, EAAT2 and EAAT5 coexist near release sites, but EAAT5 predominantly regulates temporal resolution at rod bipolar-to-AII amacrine synapses^[4]. Heterologous EAAT5 studies describe low-capacity transport and slow anion-channel gating, highlighting a distinction between EAAT5 channel-like behavior and classical glutamate uptake^[5]. For experimental applications, TBOA-based pharmacology, EAAT5 knockout retinae, and rapid glutamate uncaging in mouse rods provide practical systems to test EAAT5-dependent glutamate transport, chloride conductance, and synaptic timing^[3]^[6].

References:

[1]. Arriza JL, et al. Excitatory amino acid transporter 5, a retinal glutamate transporter coupled to a chloride conductance. Proc Natl Acad Sci U S A. 1997 Apr 15;94(8):4155-60. [Content Brief]

[2]. Lukasiewcz PD, et al. EAAT5 Glutamate Transporter-Mediated Inhibition in the Vertebrate Retina. Front Cell Neurosci. 2021 May 6;15:662859. [Content Brief]

[3]. Gehlen J, et al. Excitatory Amino Acid Transporter EAAT5 Improves Temporal Resolution in the Retina. eNeuro. 2021 Dec 10;8(6):ENEURO.0406-21.2021. [Content Brief]

[4]. Tang FS, et al. Glutamate Transporters EAAT2 and EAAT5 Differentially Shape Synaptic Transmission from Rod Bipolar Cell Terminals. eNeuro. 2022 May 18;9(3):ENEURO.0074-22.2022. [Content Brief]

[5]. Gameiro A, et al. The discovery of slowness: low-capacity transport and slow anion channel gating by the glutamate transporter EAAT5. Biophys J. 2011 Jun 8;100(11):2623-32. [Content Brief]

[6]. Thoreson WB, et al. EAAT5 glutamate transporter rapidly binds glutamate with micromolar affinity in mouse rods. J Gen Physiol. 2023 Sep 4;155(9):e202313349. [Content Brief]

EAAT5 Inhibitors (2)

EAAT5 Related Products (2):

Cat. No.	Product Name	Effect	Purity

HY-146242

SN05	Inhibitor	98.03%
SN05 is a potent amino acid transport (AAT) inhibitor with K_i values of 2.77 μM, 0.73 μM, 0.87 μM, 3.7 μM, 7.25 μM, 7.23 μM and 2.22 μM for hASCT1, rASCT2, hASCT2, EAAT1, EAAT2, EAAC1 and EAAT5, respectively. SN05 can be used in the study of cancer.

HY-146241

SN40	Inhibitor	99.26%
SN40 is a potent amino acid transport (AAT) inhibitor with K_is of 7.29 μM, 2.42 μM, 2.94 μM, 5.55 μM, 24.43 μM and 5.55 μM for rat ASCT2, human ASCT2, EAAT1, EAAT2, EAAC1 and EAAT5, respectively. SN40 can be used for researching anticancer.

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