1. Membrane Transporter/Ion Channel
  2. EAAT2
  3. DL-TBOA ammonium

DL-TBOA ammonium 

Cat. No.: HY-107522B
Handling Instructions

DL-TBOA ammonium is a potent non-transportable inhibitor of excitatory amino acid transporters with IC50s of 70 μM, 6 μM and 6 μM for excitatory amino acid transporter-1 (EAAT1), EAAT2 and EAAT3, respectively. DL-TBOA ammonium inhibits the uptake of [14C]glutamate in COS-1 cells expressing the human EAAT1 and EAAT2 with Ki valuesof 42 μM and 5.7 μM, respectively. DL-TBOA ammonium blocks EAAT4 and EAAT5 in a competitive manner with Ki values of 4.4 μM and 3.2 μM, respectively.

For research use only. We do not sell to patients.

DL-TBOA ammonium Chemical Structure

DL-TBOA ammonium Chemical Structure

CAS No. : 2093503-71-8

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Description

DL-TBOA ammonium is a potent non-transportable inhibitor of excitatory amino acid transporters with IC50s of 70 μM, 6 μM and 6 μM for excitatory amino acid transporter-1 (EAAT1), EAAT2 and EAAT3, respectively. DL-TBOA ammonium inhibits the uptake of [14C]glutamate in COS-1 cells expressing the human EAAT1 and EAAT2 with Ki valuesof 42 μM and 5.7 μM, respectively. DL-TBOA ammonium blocks EAAT4 and EAAT5 in a competitive manner with Ki values of 4.4 μM and 3.2 μM, respectively[1][2][3].

IC50 & Target

IC50: 70 μM (EAAT1), 6 μM (EAAT2) and 6 μM (EAAT3); Ki: 42 μM (human EAAT1), 5.7 μM (human EAAT2), 4.4 μM (EAAT4) and 3.2 μM (EAAT5)[1][2][3]

In Vitro

DL-TBOA ammonium (70-350 μM; 48 hours) treatment concentration-dependently enhances SN38-induced loss of viability. DL-TBOA reversed Oxaliplatin-induced loss of viability[4].
DL-TBOA ammonium (350 μM; 24 hours) decreases p53 induction by SN38 and oxaliplatin[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[4]

Cell Line: HCT116 cells, LoVo cells
Concentration: 70 μM, 350 μM
Incubation Time: 48 hours
Result: Enhanced SN38-induced, and counteracted Oxaliplatin-induced, cell death.

Cell Viability Assay[4]

Cell Line: HCT116 cells, LoVo cells
Concentration: 350 μM
Incubation Time: 24 hours
Result: p53 induction by SN38 and oxaliplatin was decreased.
In Vivo

DL-TBOA ammonium (10 nmol; i.c.v.) to morphine-dependent rats significantly facilitates the expression of naloxone-precipitated somatic signs and conditioned place aversion[5].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Sprague-Dawley rats (180-250 g)[5]
Dosage: 1 nmol, 3 nmol, 10 nmol
Administration: Intracerebroventricularly injection (i.c.v.)
Result: Dose dependently facilitated various somatic signs induced by Naloxone (0.1 mg/kg)-precipitated morphine withdrawal.
Molecular Weight

256.26

Formula

C₁₁H₁₆N₂O₅

CAS No.

2093503-71-8

SMILES

OC([[email protected]@H](N)[[email protected]@H](C(O)=O)OCC1=CC=CC=C1)=O.[Relative stereochemistry].N

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

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Keywords:

DL-TBOAEAAT2Excitatory amino acid transporter 2Glutamate transporter 1GLT-1glutamateuptakeInhibitorinhibitorinhibit

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DL-TBOA ammonium
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