WAY-213613 hydrochloride

Name: WAY-213613 hydrochloride
Brand: MedChemExpress
SKU: HY-107523A
Rating: 4.5 (3 reviews)

Cat. No.: HY-107523A Purity: 99.57%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

WAY-213613 (hydrochloride) is a potent and selective human EAAT2 inhibitor. WAY-213613 has potent EAAT2 inhibitory activity with an IC₅₀ value of 85 nM. WAY-213613 can be used for the research of central nervous system.

For research use only. We do not sell to patients.

WAY-213613 hydrochloride Chemical Structure

CAS No. : 2450268-84-3

Size	Price	Stock	Quantity
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 176	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 176	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 160	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 210	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 480	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 800	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1400	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of WAY-213613 hydrochloride:

WAY-213613 Get quote

2 Publications Citing Use of MCE WAY-213613 hydrochloride

: WAY-213613 hydrochloride purchased from MedChemExpress. Usage Cited in: Ann Transl Med. 2020 Jun;8(11):691. [Abstract]; The SAH mice are intraperitoneally administered with HDAC2 inhibitor (2 mg/kg) and GLT-1 inhibitor (WAY-213613, 1 mg/kg) two weeks after surgery once every other day. The expression of GLT-1, HDAC2, p-GluN2B and p-GluA1 in hippocampus is detected after SAH at 8 weeks.

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EAAT1 EAAT2 EAAT3 EAAT5

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

EAAT2

In Vitro

WAY-213613 (hydrochloride) (0-100 μM) has inhibitory activity for human EAAT1, EAAT2 and EAAT3 subtype with IC₅₀ values of 5004 nM, 85 nM and 3787 nM, respectively^[1].
?? WAY-213613 (3, 30, 300 nM) has the inhibitory effect on synaptosomal L-[³H] glutamate uptake with K_i values of 15 nM, 41 nM and 55 nM in the presence of 3, 30 and 300 nM, respectively^[1].
WAY-213613 (0-100 μM) produces a concentration-dependent block of glutamate-induced currents in EAAT1-, EAAT2- or EAAT3-injected oocytes, with IC₅₀ values of 48, 0.13 and 4.0 μM, respectively^[1].
WAY-213613 (0.5–50 μM) exhibits good selectivity over ionotropic receptors and EAAT2 and potent activity toward blocking NMDA-stimulated responses^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

451.65

Formula

C₁₆H₁₄BrClF₂N₂O₄

CAS No.

2450268-84-3

Appearance

Solid

Color

White to off-white

SMILES

BrC(C=C(F)C(F)=C1)=C1OC2=CC=C(NC(C[C@H](N)C(O)=O)=O)C=C2.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (110.71 mM; warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2141 mL	11.0705 mL	22.1410 mL
5 mM	0.4428 mL	2.2141 mL	4.4282 mL
10 mM	0.2214 mL	1.1071 mL	2.2141 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.54 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.54 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.54 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.57%

Select Batch:

Data Sheet (274 KB) SDS (251 KB)

COA (245 KB) RP-HPLC (99 KB)

Handling Instructions (2659 KB)

References

[1]. Dunlop J, et al. Characterization of novel aryl-ether, biaryl, and fluorene aspartic acid and diaminopropionic acid analogs as potent inhibitors of the high-affinity glutamate transporter EAAT2. Mol Pharmacol. 2005 Oct;68(4):974-82. Epub 2005 Jul 13. [Content Brief]

[2]. Simmons DA, et al. A small molecule p75NTR ligand, LM11A-31, reverses cholinergic neurite dystrophy in Alzheimer's disease mouse models with mid- to late-stage disease progression. PLoS One. 2014 Aug 25;9(8):e102136. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.2141 mL	11.0705 mL	22.1410 mL	55.3526 mL
	5 mM	0.4428 mL	2.2141 mL	4.4282 mL	11.0705 mL
	10 mM	0.2214 mL	1.1071 mL	2.2141 mL	5.5353 mL
	15 mM	0.1476 mL	0.7380 mL	1.4761 mL	3.6902 mL
	20 mM	0.1107 mL	0.5535 mL	1.1071 mL	2.7676 mL
	25 mM	0.0886 mL	0.4428 mL	0.8856 mL	2.2141 mL
	30 mM	0.0738 mL	0.3690 mL	0.7380 mL	1.8451 mL
	40 mM	0.0554 mL	0.2768 mL	0.5535 mL	1.3838 mL
	50 mM	0.0443 mL	0.2214 mL	0.4428 mL	1.1071 mL
	60 mM	0.0369 mL	0.1845 mL	0.3690 mL	0.9225 mL
	80 mM	0.0277 mL	0.1384 mL	0.2768 mL	0.6919 mL
	100 mM	0.0221 mL	0.1107 mL	0.2214 mL	0.5535 mL