1. Others
  2. Others

UCPH-101 

Cat. No.: HY-10914 Purity: 99.12%
Handling Instructions

UCPH-101 is an excitatory amino acid transporter subtype 1 (EAAT1) inhibitor with an IC50 of 0.66 μM.

For research use only. We do not sell to patients.

UCPH-101 Chemical Structure

UCPH-101 Chemical Structure

CAS No. : 1118460-77-7

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 211 In-stock
Estimated Time of Arrival: December 31
5 mg USD 192 In-stock
Estimated Time of Arrival: December 31
10 mg USD 324 In-stock
Estimated Time of Arrival: December 31
25 mg USD 648 In-stock
Estimated Time of Arrival: December 31
50 mg USD 1044 In-stock
Estimated Time of Arrival: December 31
100 mg USD 1860 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

Customer Review

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

UCPH-101 is an excitatory amino acid transporter subtype 1 (EAAT1) inhibitor with an IC50 of 0.66 μM.

IC50 & Target

IC50: 0.66 μM (EAAT1)[1]

In Vitro

UCPH-101 and UCPH-102 inhibit EAAT1 anion currents in a concentration-dependent manner, with KD values of 0.34±0.03 μM (Hill=1.3±0.13, n≥9) for UCPH-101 and 0.17±0.02 μM (Hill=0.97±0.11, n≥7) for UCPH-102. A small but significant decrease in the total expression levels of both HA-EAAT1 and HA-GLAST is observed in cells preincubated with 100 μM UCPH-101 (p=0.048)[1].

Solvent & Solubility
In Vitro: 

DMSO : ≥ 110 mg/mL (260.37 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3670 mL 11.8349 mL 23.6698 mL
5 mM 0.4734 mL 2.3670 mL 4.7340 mL
10 mM 0.2367 mL 1.1835 mL 2.3670 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay
[1]

Cells are split into poly-D-lysine-coated black 96-well plates with clear bottom. At 16 to 24 h later, the medium is aspirated, and the cells are washed with 100 μL Krebs buffer [140 mM NaCl/4.7 mM KCl/2.5 mM CaCl2/1.2 mM MgCl2/11 mM HEPES/10 mM D-glucose, pH 7.4]; 50 μL Krebs buffer supplemented with various concentrations of UCPH-101 or TBOA is added to the wells, after which an additional 50 μL Krebs buffer supplemented with the FMP assay dye (1 mg/mL) is added to each well. The plate is incubated at 37°C in a humidified 5% CO2 incubator for 30 min and assayed in a reader measuring emission at 560 nm caused by excitation at 530 nm before and up to 1 min after addition of 33 μL Glu solution[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

422.48

Formula

C₂₇H₂₂N₂O₃

CAS No.

1118460-77-7

SMILES

O=C(C1)C2=C(OC(N)=C(C#N)C2C3=CC=C(OC)C=C3)CC1C4=CC=CC5=C4C=CC=C5

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Inquiry Online

Your information is safe with us. * Required Fields.

Product name

 

Salutation

Applicant name *

 

Email address *

Phone number *

 

Organization name *

Country or Region *

 

Requested quantity *

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
UCPH-101
Cat. No.:
HY-10914
Quantity:

UCPH-101

Cat. No.: HY-10914