2. Cell Cycle/DNA Damage
    Apoptosis
    Anti-infection
  3. DNA/RNA Synthesis
    Topoisomerase
    Apoptosis
    Antibiotic
  4. Epirubicin hydrochloride

Epirubicin hydrochloride  (Synonyms: 4'-Epidoxorubicin hydrochloride)

Cat. No.: HY-13624A Purity: 99.04%
COA Handling Instructions

Epirubicin hydrochloride (4'-Epidoxorubicin hydrochloride), a semisynthetic L-arabino derivative of doxorubicin, has an antineoplastic agent by inhibiting Topoisomerase. Epirubicin hydrochloride inhibits DNA and RNA synthesis. Epirubicin hydrochloride is a Forkhead box protein p3 (Foxp3) inhibitor and inhibits regulatory T cell activity.

For research use only. We do not sell to patients.

Epirubicin hydrochloride Chemical Structure

Epirubicin hydrochloride Chemical Structure

CAS No. : 56390-09-1

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Solution
10 mM * 1 mL in DMSO USD 85 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
 USD 85 In-stock
Estimated Time of Arrival: December 31
Solid
5 mg USD 66 In-stock
Estimated Time of Arrival: December 31
10 mg USD 106 In-stock
Estimated Time of Arrival: December 31
50 mg USD 317 In-stock
Estimated Time of Arrival: December 31
100 mg USD 422 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 17 publication(s) in Google Scholar

Other Forms of Epirubicin hydrochloride:

Top Publications Citing Use of Products

    Epirubicin hydrochloride purchased from MCE. Usage Cited in: Asia Pac J Clin Oncol. 2022 Dec 4.  [Abstract]

    The Epirubicin (EPI) + iodine-125 (125I) group is significantly decreases the proliferation ability of HepG2 and SMMC7721 cells (cells are cultured for 2-3 weeks).

    Epirubicin hydrochloride purchased from MCE. Usage Cited in: Asia Pac J Clin Oncol. 2022 Dec 4.  [Abstract]

    Epirubicin (EPI) promotes iodine-125 (125I)-induced downregulation of the WNT pathway and enhances the radiosensitivity of HepG2 and SMMC7721 cells.

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    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Epirubicin hydrochloride (4'-Epidoxorubicin hydrochloride), a semisynthetic L-arabino derivative of doxorubicin, has an antineoplastic agent by inhibiting Topoisomerase[1]. Epirubicin hydrochloride inhibits DNA and RNA synthesis. Epirubicin hydrochloride is a Forkhead box protein p3 (Foxp3) inhibitor and inhibits regulatory T cell activity[2].

    IC50 & Target[1]

    Topoisomerase

     

    In Vitro

    Epirubicin hydrochloride (4'-Epidoxorubicin hydrochloride), like doxorubicin, exerts its antitumor effects by complex with DNA, resulting in damage to DNA and interference with the synthesis of DNA, RNA, and proteins. Epirubicin hydrochloride may also affect the integrity and activity of cellular membranes. Maximal cell kill caused by Epirubicin hydrochloride occurs during the S phase of the cell cycle. With higher concentrations effects are also seen in early G2 as well as G1 and M phases[1].
    Epirubicin hydrochloride display antineoplastic activity against most cancer cells. Epirubicin hydrochloride is cytotoxic to Hepatoma G2 cells with IC50 of 1.6 μg/mL at 24 hr. 1.6 μg/mL Epirubicin hydrochloride induces apoptosis of Hep G2 cells, and higher activity of catalase by 50%, Se-dependent glutathione peroxidase by 110%, Cu, Zn-superoxide dismutase by 172% and Mn-superoxide dismutase by 135%. Epirubicin hydrochloride increases the cellular expression of NADPH-CYP 450 reductase, and reduces GST-π expression[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    In Vivo

    Epirubicin hydrochloride (4'-Epidoxorubicin hydrochloride) are clinically active against a broad range of tumor types, including breast cancer, malignant lymphomas, soft tissue sarcomas, lung cancer, pleural mesothelioma, gastrointestinal cancer, head and neck cancer, ovarian cancer, prostatic carcinoma, transitional bladder carcinoma and so on[4].
    Epirubicin hydrochloride at a dose of 3.5 mg/kg suppresses tumor mass of human breast tumor xenograft R-27 by 74.4 %[5].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    Molecular Weight

    579.98

    Appearance

    Solid

    Formula

    C27H30ClNO11
    CAS No.
    SMILES

    O=C(C1=C2C(O)=C3[[email protected]@H](O[[email protected]@]4([H])C[[email protected]](N)[[email protected]@H](O)[[email protected]](C)O4)C[[email protected]@](C(CO)=O)(O)CC3=C1O)C5=CC=CC(OC)=C5C2=O.Cl
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
    Solvent & Solubility
    In Vitro: 

    H2O : 50 mg/mL (86.21 mM; Need ultrasonic)

    DMSO : 25 mg/mL (43.10 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.7242 mL 8.6210 mL 17.2420 mL
    5 mM 0.3448 mL 1.7242 mL 3.4484 mL
    10 mM 0.1724 mL 0.8621 mL 1.7242 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  5% DMSO    40% PEG300    5% Tween-80    50% saline

      Solubility: ≥ 2.5 mg/mL (4.31 mM); Clear solution

    • 2.

      Add each solvent one by one:  5% DMSO    95% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.5 mg/mL (4.31 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.08 mg/mL (3.59 mM); Clear solution

    • 4.

      Add each solvent one by one:  PBS

      Solubility: 1.1 mg/mL (1.90 mM); Clear solution; Need ultrasonic

    • 5.

      Add each solvent one by one:  1% DMSO    99% saline

      Solubility: ≥ 0.5 mg/mL (0.86 mM); Clear solution

    *All of the co-solvents are available by MCE.
    Purity & Documentation

    Purity: 99.16%

    COA (261 KB) HNMR (227 KB) LCMS (120 KB)

    Handling Instructions (2659 KB)
    References
    Cell Assay
    [3]

    Hep G2 cells (500 cells/well, monolayer) are plated in a 96-well plate. The next day the cells are treated with Epirubicin in the medium. At the end of the incubation periods, 15% volume of MTT dye solution is added. After 1 hr of incubation at 37°C, an equal volume of solubilization/stop solution (dimethylsul-foxide) is added to each well for an additional 1 hr incubation. The absorbance of the reaction solution at 570 nm is recorded.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References
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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Keywords:

    Epirubicin56390-09-14'-EpidoxorubicinDNA/RNA SynthesisTopoisomeraseApoptosisAntibioticL-arabinoderivativeFoxp3TcellInhibitorinhibitorinhibit

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