2. Metabolic Enzyme/Protease
  3. Fatty Acid Synthase (FASN)
  4. Fatostatin hydrobromide

Fatostatin (hydrobromide)  (Synonyms: 125B11 (hydrobromide))

製品番号: HY-14452A 純度: 99.52%
COA 取扱説明書 Technical Support

Fatostatin hydrobromide (125B11 hydrobromide), a specific inhibitor of SREBP activation, impairs the activation of SREBP-1 and SREBP-2. Fatostatin hydrobromide binds to SCAP (SREBP cleavage-activating protein), and inhibits the ER-Golgi translocation of SREBPs. Fatostatin hydrobromide decreases the transcription of lipogenic genes in cells. Fatostatin hydrobromide possesses antitumor properties, and lowers hyperglycemia in ob/ob mice.

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CAS 番号 : 298197-04-3

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>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 60 在庫あり
Solution
10 mM * 1 mL in DMSO USD 60 在庫あり
Solid
5 mg $55 在庫あり
10 mg $86 在庫あり
25 mg $160 在庫あり
50 mg $270 在庫あり
100 mg $462 在庫あり
200 mg $690 在庫あり
1 g $1200 在庫あり
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カスタマーレビュー

Based on 52 publication(s) in Google Scholar

Other Forms of Fatostatin hydrobromide:

Fatostatin (hydrobromide) 関連抗体

Top Publications Citing Use of Products

顧客検証

2D/3D Cell Culture and Differentiation
WB
Cell Proliferation/Viability Assay

    Fatostatin hydrobromide purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 25;16(1):1774.  [Abstract]

    Viable cell numbers and PUFA-PC ratio after treatment with LY294002 (iPI3K, 10 µM), Picropodophyllin (iRTK, 10 µM), Ipatasertib (iAkt, 10 µM), or Fatostatin (iSREBP1, 20 µM) for 48 h.

    Fatostatin hydrobromide purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2024 Sep 26:S1535-6108(24)00350-7.  [Abstract]

    Representative images of rat mammary organoids treated with Midkine (300 ng/mL), Betulin (5 μM), Fatostatin (5 μM) and Rapamycin (25 nM) for 5 days.

    Fatostatin hydrobromide purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2024 Sep 26:S1535-6108(24)00350-7.  [Abstract]

    Immunoblot showing the expression of MDK, mature SREBF1 (SREBF1 (M)), S6, p-S6(Ser235/236), AKT, p-AKT(Ser473) in the organoids treated with Midkine (300 ng/mL), Betulin (5 μM), Fatostatin (5 μM) and Rapamycin (25 nM) for 5 days. Tubulin serves as loading control.

    Fatostatin hydrobromide purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2022 Oct 28;8(1):431.  [Abstract]

    NUPR1 overexpression‐induced lipogenic enzymes expression can be suppressed by co-incubation with Fatostatin (20 μM; 48 h) in MHCC-97H and SK-Hep1 cells.

    Fatostatin hydrobromide purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2022 Oct 28;8(1):431.  [Abstract]

    Transwell migration assay. Fatostatin reduces the migration ability of NUPR1-overexpressing MHCC-97H cells.

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    製品説明

    Fatostatin hydrobromide (125B11 hydrobromide), a specific inhibitor of SREBP activation, impairs the activation of SREBP-1 and SREBP-2. Fatostatin hydrobromide binds to SCAP (SREBP cleavage-activating protein), and inhibits the ER-Golgi translocation of SREBPs. Fatostatin hydrobromide decreases the transcription of lipogenic genes in cells. Fatostatin hydrobromide possesses antitumor properties, and lowers hyperglycemia in ob/ob mice[1][2].

    体外実験

    Fatostatin hydrobromide (125B11 hydrobromide) (0.1-1 μM; 3 days) inhibits the androgen-independent prostate cancer cell proliferation (IC50=0.1 μM) in an independent of the known IGF1-signaling pathway. Fatostatin hydrobromide inhibits insulin-induced adipogenesis of 3T3-L1 cells[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Fatostatin (hydrobromide) 関連抗体

    Cell Proliferation Assay[1]

    Cell Line: DU-145 cells
    Concentration: 0.1, 1 μM
    Incubation Time: 3 days
    Result: Impaired the IGF1-induced growth at an IC50 of 0.1 μM.
    体内実験

    Fatostatin hydrobromide (125B11 hydrobromide) (30 mg/kg; 150 mL; i.p.; daily for 28 days) reduces adiposity, ameliorates fatty liver by reducing triglyceride (TG) storage, and lowers hyperglycemia in ob/ob mice[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Four-to-five-week-old homozygous male obese (ob/ob) mice (C57BL/6J)[2]
    Dosage: 30 mg/kg; 150 mL
    Administration: i.p. injection; daily for 28 days
    Result: Blocked increases in body weight, blood glucose, and hepatic fat accumulation in obese ob/ob mice, even under uncontrolled food intake.
    分子量

    375.33

    分子式

    C18H19BrN2S
    CAS 番号
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    CCCC1=NC=CC(C2=NC(C3=CC=C(C)C=C3)=CS2)=C1.[H]Br
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
    溶剤 & 溶解度
    体外: 

    DMSO : 25 mg/mL (66.61 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.6643 mL 13.3216 mL 26.6432 mL
    5 mM 0.5329 mL 2.6643 mL 5.3286 mL
    10 mM 0.2664 mL 1.3322 mL 2.6643 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

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    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (5.54 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: 1 mg/mL (2.66 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 1 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.6643 mL 13.3216 mL 26.6432 mL 66.6081 mL
    5 mM 0.5329 mL 2.6643 mL 5.3286 mL 13.3216 mL
    10 mM 0.2664 mL 1.3322 mL 2.6643 mL 6.6608 mL
    15 mM 0.1776 mL 0.8881 mL 1.7762 mL 4.4405 mL
    20 mM 0.1332 mL 0.6661 mL 1.3322 mL 3.3304 mL
    25 mM 0.1066 mL 0.5329 mL 1.0657 mL 2.6643 mL
    30 mM 0.0888 mL 0.4441 mL 0.8881 mL 2.2203 mL
    40 mM 0.0666 mL 0.3330 mL 0.6661 mL 1.6652 mL
    50 mM 0.0533 mL 0.2664 mL 0.5329 mL 1.3322 mL
    60 mM 0.0444 mL 0.2220 mL 0.4441 mL 1.1101 mL
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    Fatostatin hydrobromide Related Classifications

    • Molarity Calculator

    • Dilution Calculator

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    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Fatostatin298197-04-3125B11Fatty Acid Synthase (FASN)GolgiSREBP-mediatedgeneexpressionhumanprostatecancerinsulin-inducedadipogenesisob/obmiceInhibitorinhibitorinhibit

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    製品名:
    Fatostatin hydrobromide
    製品番号:
    HY-14452A
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