2. Membrane Transporter/Ion Channel
  3. Na+/K+ ATPase
  4. Gitoxin

Gitoxin is a degradation metabolite of Digitoxin (HY-B1357) and a non-competitive Na+/K+-ATPase inhibitor, with an IC50 of 1.18e-6 M against the porcine high-affinity subtype and an IC50 of 2.85e-5 M against the porcine low-affinity subtype. Gitoxin regulates atrial contractility and rhythmicity. Gitoxin is applicable to research related to congestive heart failure.

For research use only. We do not sell to patients.

Gitoxin

Gitoxin Chemical Structure

CAS No. : 4562-36-1

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Other Forms of Gitoxin:

Gitoxin Related Antibodies

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Description

Gitoxin is a degradation metabolite of Digitoxin (HY-B1357) and a non-competitive Na+/K+-ATPase inhibitor, with an IC50 of 1.18e-6 M against the porcine high-affinity subtype and an IC50 of 2.85e-5 M against the porcine low-affinity subtype. Gitoxin regulates atrial contractility and rhythmicity. Gitoxin is applicable to research related to congestive heart failure[1][2].

Cellular Effect
Cell Line Type Value Description References
MCF7 IC50
251.9 nM
Compound: 5
Cytotoxicity against human MCF7 cells after 48 hrs by SRB assay
Cytotoxicity against human MCF7 cells after 48 hrs by SRB assay
[PMID: 16309315]
TK-10 IC50
131.2 nM
Compound: 5
Cytotoxicity against human TK10 cells after 48 hrs by SRB assay
Cytotoxicity against human TK10 cells after 48 hrs by SRB assay
[PMID: 16309315]
UACC-62 IC50
369.4 nM
Compound: 5
Cytotoxicity against human UACC62 cells after 48 hrs by SRB assay
Cytotoxicity against human UACC62 cells after 48 hrs by SRB assay
[PMID: 16309315]
In Vitro

Gitoxin (60 min after a 10 min pre-incubation) exerts a biphasic inhibitory effect on erythrocyte membrane Na+/K+-ATPase, with stronger inhibitory potency against the high-affinity subtype (IC50 = 2.98 × 10-7 M), and it exhibits positive cooperative binding with this enzyme[1].
Gitoxin (60 min after 10 min pre-incubation) exerts biphasic inhibition on Na+/K+-ATPase from porcine cerebral cortex, with stronger inhibitory potency against the high-affinity isoform (IC50 = 1.18 × 10-6 M), and it exhibits positive cooperative binding with this enzyme[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Gitoxin Related Antibodies
Molecular Weight

780.94

Formula

C41H64O14
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O[C@@]([C@@]([C@@]1([H])C(CO2)=CC2=O)(CC3)C)(C[C@@H]1O)[C@@](CC[C@]4([H])C[C@H]5O[C@H](O[C@@H]6C)C[C@@H]([C@@H]6O[C@H](O[C@@H]7C)C[C@@H]([C@@H]7O[C@H](O[C@@H]8C)C[C@@H]([C@@H]8O)O)O)O)([H])[C@@]3([H])[C@]4(CC5)C
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

Ethanol : 8 mg/mL (10.24 mM; Need ultrasonic and warming)

DMF : 3.73 mg/mL (4.78 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2805 mL 6.4025 mL 12.8051 mL
5 mM 0.2561 mL 1.2805 mL 2.5610 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Working solution concentration: mg/mL
Purity & Documentation

Purity: 95.00%

SDS (480 KB)

COA (249 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMF / Ethanol 1 mM 1.2805 mL 6.4025 mL 12.8051 mL 32.0127 mL
Ethanol 5 mM 0.2561 mL 1.2805 mL 2.5610 mL 6.4025 mL
10 mM 0.1281 mL 0.6403 mL 1.2805 mL 3.2013 mL
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Gitoxin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Gitoxin4562-36-1Na+/K+ ATPaseSodium potassium pumpenzyme-substrate complexesatrial rhythmicityhuman erythrocyte membraneβ-receptor blockadedigoxinporcine cerebral cortexNa+/K+-ATPaseextracellular potassiumcongestive heart failureatrial contractilityInhibitorinhibitorinhibit

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