Guanosine 5'-triphosphate-5'-adenosine  (Synonyms: GpppA)

Guanosine 5'-triphosphate-5'-adenosine (GpppA), a 5′ cap analog, can be used for RNA synthesis in vitro. Guanosine 5'-triphosphate-5'-adenosine is a fluorescent substrate analog^[1][2].

Guanosine 5'-triphosphate-5'-adenosine (GpppA) labeled with pyrene at the 3′‐O position of adenosine acts as an artificial substrate. Fluorescently labeled Guanosine 5'-triphosphate-5'-adenosine (GpppA) and GpppG analogs as potential substrates that represent a reasonable compromise between the structural complexity and requirements of the enzyme^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

772.41

C₂₀H₂₇N₁₀O₁₇P₃

10527-47-6

O=P(O)(OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C=NC3=C(N)N=CN=C23)O1)OP(OP(OC[C@@H]4[C@@H](O)[C@@H](O)[C@H](N5C(NC(N)=N6)=C(N=C5)C6=O)O4)(O)=O)(O)=O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Renata Kasprzyk, et al. Direct High-Throughput Screening Assay for mRNA Cap Guanine-N7 Methyltransferase Activity. Chemistry. 2020 Sep 1;26(49):11266-11275.  [Content Brief]

  [2]. Dennis Reichert, et al. Light-control of cap methylation and mRNA translation via genetic code expansion of Ecm1. Chem Sci. 2021 Feb 8;12(12):4383-4388.  [Content Brief]