Guanosine 5'-triphosphate-5'-adenosine triammonium
Based on 1 Customer Validation
Guanosine 5'-triphosphate-5'-adenosine (GpppA) triammonium, a 5′ cap analog, can be used for RNA synthesis in vitro. Guanosine 5'-triphosphate-5'-adenosine triammonium is a fluorescent substrate analog.
For research use only. We do not sell to patients.
- Purity : 99.96%
- Formula: C20H36N13O17P3
- Molecular Weight:823.50
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Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
Human Endogenous Metabolite |
In Vitro
Guanosine 5'-triphosphate-5'-adenosine (GpppA) triammonium labeled with pyrene at the 3′-O position of adenosine acts as an artificial substrate. Fluorescently labeled GpppA triammonium and GpppG analogs as potential substrates that represent a reasonable compromise between the structural complexity and requirements of the enzyme[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Solid
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Molecular Weight 823.50
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Formula C20H36N13O17P3
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Color White to off-white
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SMILES
O=P(O)(OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C=NC3=C(N)N=CN=C23)O1)OP(OP(OC[C@@H]4[C@@H](O)[C@@H](O)[C@H](N5C(NC(N)=N6)=C(N=C5)C6=O)O4)(O)=O)(O)=O.N.N.N
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Synonyms
GpppA triammonium
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro:
H2O : ≥ 125 mg/mL (151.79 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (285 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Renata Kasprzyk, et al. Direct High-Throughput Screening Assay for mRNA Cap Guanine-N7 Methyltransferase Activity. Chemistry. 2020 Sep 1;26(49):11266-11275. [Content Brief]
[2]. Dennis Reichert, et al. Light-control of cap methylation and mRNA translation via genetic code expansion of Ecm1. Chem Sci. 2021 Feb 8;12(12):4383-4388. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.2143 mL | 6.0716 mL | 12.1433 mL | 30.3582 mL |
| 5 mM | 0.2429 mL | 1.2143 mL | 2.4287 mL | 6.0716 mL | |
| 10 mM | 0.1214 mL | 0.6072 mL | 1.2143 mL | 3.0358 mL | |
| 15 mM | 0.0810 mL | 0.4048 mL | 0.8096 mL | 2.0239 mL | |
| 20 mM | 0.0607 mL | 0.3036 mL | 0.6072 mL | 1.5179 mL | |
| 25 mM | 0.0486 mL | 0.2429 mL | 0.4857 mL | 1.2143 mL | |
| 30 mM | 0.0405 mL | 0.2024 mL | 0.4048 mL | 1.0119 mL | |
| 40 mM | 0.0304 mL | 0.1518 mL | 0.3036 mL | 0.7590 mL | |
| 50 mM | 0.0243 mL | 0.1214 mL | 0.2429 mL | 0.6072 mL | |
| 60 mM | 0.0202 mL | 0.1012 mL | 0.2024 mL | 0.5060 mL | |
| 80 mM | 0.0152 mL | 0.0759 mL | 0.1518 mL | 0.3795 mL | |
| 100 mM | 0.0121 mL | 0.0607 mL | 0.1214 mL | 0.3036 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Guanosine 5'-triphosphate-5'-adenosine triammonium Related Classifications
HY-139101A Related Classifications
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