Guanosine 5'-triphosphate-5'-adenosine triammonium (Synonyms: GpppA triammonium)

Cat. No.: HY-139101A: FAQs
Data Sheet Handling Instructions

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Guanosine 5'-triphosphate-5'-adenosine (GpppA) triammonium, a 5′ cap analog, can be used for RNA synthesis in vitro. Guanosine 5'-triphosphate-5'-adenosine triammonium is a fluorescent substrate analog.

For research use only. We do not sell to patients.

Guanosine 5'-triphosphate-5'-adenosine triammonium Chemical Structure

Size	Price	Stock	Quantity
5 mg	USD 330	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 530	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 1050	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1650	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 2600	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Other Forms of Guanosine 5'-triphosphate-5'-adenosine triammonium:

Guanosine 5'-triphosphate-5'-adenosine Get quote

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Biological Activity
Purity & Documentation
References
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Description

IC₅₀ & Target^[1]

Human Endogenous Metabolite

In Vitro

Guanosine 5'-triphosphate-5'-adenosine (GpppA) triammonium labeled with pyrene at the 3′-O position of adenosine acts as an artificial substrate. Fluorescently labeled GpppA triammonium and GpppG analogs as potential substrates that represent a reasonable compromise between the structural complexity and requirements of the enzyme^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

823.50

Formula

C₂₀H₃₆N₁₃O₁₇P₃

Appearance

Solid

SMILES

O=P(O)(OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C=NC3=C(N)N=CN=C23)O1)OP(OP(OC[C@@H]4[C@@H](O)[C@@H](O)[C@H](N5C(NC(N)=N6)=C(N=C5)C6=O)O4)(O)=O)(O)=O.N.N.N

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : ≥ 125 mg/mL (151.79 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.2143 mL	6.0716 mL	12.1433 mL
5 mM	0.2429 mL	1.2143 mL	2.4287 mL
10 mM	0.1214 mL	0.6072 mL	1.2143 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
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Dosing volume
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μL

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Renata Kasprzyk, et al. Direct High-Throughput Screening Assay for mRNA Cap Guanine-N7 Methyltransferase Activity. Chemistry. 2020 Sep 1;26(49):11266-11275. [Content Brief]

[2]. Dennis Reichert, et al. Light-control of cap methylation and mRNA translation via genetic code expansion of Ecm1. Chem Sci. 2021 Feb 8;12(12):4383-4388. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	1.2143 mL	6.0716 mL	12.1433 mL	30.3582 mL
	5 mM	0.2429 mL	1.2143 mL	2.4287 mL	6.0716 mL
	10 mM	0.1214 mL	0.6072 mL	1.2143 mL	3.0358 mL
	15 mM	0.0810 mL	0.4048 mL	0.8096 mL	2.0239 mL
	20 mM	0.0607 mL	0.3036 mL	0.6072 mL	1.5179 mL
	25 mM	0.0486 mL	0.2429 mL	0.4857 mL	1.2143 mL
	30 mM	0.0405 mL	0.2024 mL	0.4048 mL	1.0119 mL
	40 mM	0.0304 mL	0.1518 mL	0.3036 mL	0.7590 mL
	50 mM	0.0243 mL	0.1214 mL	0.2429 mL	0.6072 mL
	60 mM	0.0202 mL	0.1012 mL	0.2024 mL	0.5060 mL
	80 mM	0.0152 mL	0.0759 mL	0.1518 mL	0.3795 mL
	100 mM	0.0121 mL	0.0607 mL	0.1214 mL	0.3036 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Guanosine 5'-triphosphate-5'-adenosine triammonium Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Guanosine 5'-triphosphate-5'-adenosine triammoniumGpppA triammoniumDNA/RNA SynthesisEndogenous MetaboliteInhibitorinhibitorinhibit

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