Diadenosine pentaphosphate pentasodium

Cat. No.: HY-113273A Purity: 99.13%: Data Sheet
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Diadenosine pentaphosphate pentasodium is an agonist and negative modulator of the P2X1 receptor, an endogenous vasoactive purine dinucleotide that can be isolated from platelets. Diadenosine pentaphosphate pentasodium mediates negative regulation of dendrite growth and number by activating homologous and heterologous P2X1 receptors, which triggers a transient and moderate increase in intracellular calcium levels within dendritic growth cones. Diadenosine pentaphosphate pentasodium is widely present in secretory vesicles such as platelets, chromaffin cells and brain synaptosomes, and exhibits selective activity on dendrite growth of cultured hippocampal neurons, inhibiting only dendrite growth without affecting axon growth. Diadenosine pentaphosphate pentasodium has a weaker ability to compete with RcCHAD for binding to polyP than short-chain polyPs.

For research use only. We do not sell to patients.

Diadenosine pentaphosphate pentasodium Chemical Structure

CAS No. : 4097-04-5

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	Get quote
50 mg	Get quote

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Other Forms of Diadenosine pentaphosphate pentasodium:

Diadenosine pentaphosphate pentaammonium In-stock
Ap5A Get quote
Diadenosine pentaphosphate pentalithium Get quote

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P2X1 Receptor P2X2 Receptor P2X3 Receptor P2X4 Receptor P2X7 Receptor

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

p2x1 Receptor

In Vitro

Diadenosine pentaphosphate pentasodium (30 μM; 5 min+15 min) inhibits adenylate kinase activity in mitochondria isolated from wheat (Triticum aestivum v. Westonia), thereby enabling the specific detection of ATP synthase-dependent ATP synthesis^[1].
Diadenosine pentaphosphate pentasodium (100 μM; 72 h) reduces the total dendritic length and number of dendrites in E18 mouse hippocampal neurons cultured in a phenol red-free system; this effect is blocked by treatment with 10 nM NF449 (HY-112461) or 10 nM Ip5I, but not by 20 nM NF279 (HY-D0976)^[3].
Diadenosine pentaphosphate pentasodium (100 μM; 72 h) induces reductions in dendritic length and number in E18 mouse hippocampal neurons cultured in phenol red-free medium, and this effect is blocked by pretreatment with 10 nM TNP-ATP^[3].
Diadenosine polyphosphates (ApnA, n=2-7) are identified as components of secretory vesicles, such as platelets, chromaffin cells, Torpedo nerve terminals and brain synaptosomes^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Differentiation Assay^[3]

Cell Line:	E18 mouse hippocampal neurons
Concentration:	100 μM; with or without 10 nM NF449 or 10 nM Ip₅I
Incubation Time:	72 h
Result:	Reduceed total dendrite length by 28.7% and dendrite number by 28.1% in phenol red-free cultures of E18 mouse hippocampal neurons after 72 h of incubation, with no effect on axonal length. Blocked by pre-treatment with 10 nM NF449 (HY-112461) or 10 nM Ip5I, but not by 20 nM NF279 (HY-D0976).

Molecular Weight

1026.28

Formula

C₂₀H₂₄N₁₀Na₅O₂₂P₅

CAS No.

4097-04-5

Appearance

Solid

Color

White to off-white

SMILES

O[C@H]1[C@@H](O)[C@H](N2C3=NC=NC(N)=C3N=C2)O[C@@H]1COP(OP(OP(OP(OP(OC[C@@H]4[C@@H](O)[C@@H](O)[C@H](N5C6=NC=NC(N)=C6N=C5)O4)(O[Na])=O)(O[Na])=O)(O[Na])=O)(O[Na])=O)(O[Na])=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

H₂O : 87.5 mg/mL (85.26 mM; Need ultrasonic)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.9744 mL	4.8720 mL	9.7439 mL
5 mM	0.1949 mL	0.9744 mL	1.9488 mL
10 mM	0.0974 mL	0.4872 mL	0.9744 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.13%

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Data Sheet (278 KB) SDS (252 KB)

COA (252 KB) HNMR (249 KB) CNMR (316 KB) RP-HPLC (275 KB)

Handling Instructions (2659 KB)

References

[1]. Jacoby RP, et al. Analysis of the sodium chloride-dependent respiratory kinetics of wheat mitochondria reveals differential effects on phosphorylating and non-phosphorylating electron transport pathways. Plant Cell Environ. 2016;39(4):823-833.

[2]. Lorenzo-Orts L, et al. Molecular characterization of CHAD domains as inorganic polyphosphate-binding modules. Life Sci Alliance. 2019;2(3):e201900385. Published 2019 May 27.

[3]. Diez-Zaera M, et al. Diadenosine pentaphosphate regulates dendrite growth and number in cultured hippocampal neurons. Purinergic Signal. 2024;20(2):115-125.

[4]. L Giraldez, et al. Adenosine triphosphate and diadenosine pentaphosphate induce [Ca(2+)](i) increase in rat basal ganglia aminergic terminals. J Neurosci Res. 2001 Apr 15;64(2):174-82. [Content Brief]

[5]. Jesús Pintor, et al. Presence of diadenosine polyphosphates in human tears. Pflugers Arch. 2002 Jan;443(3):432-6. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	0.9744 mL	4.8720 mL	9.7439 mL	24.3598 mL
	5 mM	0.1949 mL	0.9744 mL	1.9488 mL	4.8720 mL
	10 mM	0.0974 mL	0.4872 mL	0.9744 mL	2.4360 mL
	15 mM	0.0650 mL	0.3248 mL	0.6496 mL	1.6240 mL
	20 mM	0.0487 mL	0.2436 mL	0.4872 mL	1.2180 mL
	25 mM	0.0390 mL	0.1949 mL	0.3898 mL	0.9744 mL
	30 mM	0.0325 mL	0.1624 mL	0.3248 mL	0.8120 mL
	40 mM	0.0244 mL	0.1218 mL	0.2436 mL	0.6090 mL
	50 mM	0.0195 mL	0.0974 mL	0.1949 mL	0.4872 mL
	60 mM	0.0162 mL	0.0812 mL	0.1624 mL	0.4060 mL
	80 mM	0.0122 mL	0.0609 mL	0.1218 mL	0.3045 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.