Diadenosine pentaphosphate pentaammonium
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Diadenosine pentaphosphate (Ap5A; P1,P5-Di(adenosine-5'-)pentaphosphate) pentaammonium is an endogenous generated diadenosine polyphosphate, possessing the dual identities of intracellular alarm signal and extracellular signaling molecule. Diadenosine pentaphosphate regulates calcium signaling in the nervous, cardiac and peripheral sensory systems by activating P2X1/P2X3/P2Y receptor and RyR2 channels (EC50 = 140 μM). Diadenosine pentaphosphate can be used in studies related to intracellular calcium release channels, cardiovascular diseases and nociception (pain).
For research use only. We do not sell to patients.
- Purity : 99.36%
- CAS No.: 102783-61-9
- Formula: C20H44N15O22P5
- Molecular Weight:1001.52
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Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
All Endogenous Metabolite Isoforms
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Biological Activity
Description
IC50 & Target
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Human Endogenous Metabolite |
In Vitro
Diadenosine pentaphosphate (100 μM) pentaammonium elevates the intracellular calcium level of cultured human fetal astrocytes to 980 nM via P2 purinergic receptor-mediated extracellular calcium influx, as well as calcium release from IP3-sensitive and ryanodine-sensitive intracellular calcium stores[1].
Diadenosine pentaphosphate (100 μM; 5 sec) pentaammonium increases the production of IP3 in cultured human fetal astrocytes by approximately 2-fold[1].
Diadenosine pentaphosphate (10 μM) pentaammonium activates the IKACh current in isolated guinea pig atrial myocytes by stimulating P1 receptors[2].
Diadenosine pentaphosphate (100 μM) pentaammonium induces local, transient increases in intracellular calcium in distal dendrites and dendritic growth cones of E18 mouse hippocampal neurons cultured in vitro, with no effect on calcium levels in axons or somatodendritic regions[5].
Diadenosine pentaphosphate (100 μM; 72 h) pentaammonium exerts a negative regulatory effect on dendrite growth and quantity in E18 mouse hippocampal neurons cultured in vitro (in phenol red-free medium). After treatment with 100 μM for 72 h, it reduces the total dendrite length by 28.7% and the number of dendrites by 28.1%, but has no effect on axon length[5].
Diadenosine pentaphosphate (100 μM) pentaammonium reduces dendritic length and number in cultured E18 mouse hippocampal neurons. Overexpression of P2X1 mimics this effect, while shRNA knockdown of P2X1 reverses the reduction in dendrite number but fails to rescue the decrease in dendrite length[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 102783-61-9
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Appearance Solid
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Molecular Weight 1001.52
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Formula C20H44N15O22P5
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Color Off-white to light yellow
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SMILES
O[C@H]1[C@@H](O)[C@H](N2C3=NC=NC(N)=C3N=C2)O[C@@H]1COP(OP(OP(OP(OP(OC[C@@H]4[C@@H](O)[C@@H](O)[C@H](N5C6=NC=NC(N)=C6N=C5)O4)(O)=O)(O)=O)(O)=O)(O)=O)(O)=O.N.N.N.N.N
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Synonyms
Ap5A pentaammonium; P1,P5-Di(adenosine-5'-)pentaphosphate pentaammonium
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro:
H2O : 50 mg/mL (49.92 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (277 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. L Giraldez, et al. Adenosine triphosphate and diadenosine pentaphosphate induce [Ca(2+)](i) increase in rat basal ganglia aminergic terminals. J Neurosci Res. 2001 Apr 15;64(2):174-82. [Content Brief]
[2]. Jesús Pintor, et al. Presence of diadenosine polyphosphates in human tears. Pflugers Arch. 2002 Jan;443(3):432-6. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| H2O | 1 mM | 0.9985 mL | 4.9924 mL | 9.9848 mL | 24.9621 mL |
| 5 mM | 0.1997 mL | 0.9985 mL | 1.9970 mL | 4.9924 mL | |
| 10 mM | 0.0998 mL | 0.4992 mL | 0.9985 mL | 2.4962 mL | |
| 15 mM | 0.0666 mL | 0.3328 mL | 0.6657 mL | 1.6641 mL | |
| 20 mM | 0.0499 mL | 0.2496 mL | 0.4992 mL | 1.2481 mL | |
| 25 mM | 0.0399 mL | 0.1997 mL | 0.3994 mL | 0.9985 mL | |
| 30 mM | 0.0333 mL | 0.1664 mL | 0.3328 mL | 0.8321 mL | |
| 40 mM | 0.0250 mL | 0.1248 mL | 0.2496 mL | 0.6241 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Diadenosine pentaphosphate pentaammonium Related Classifications
HY-113273B Related Classifications
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Keywords
- Diadenosine pentaphosphate pentaammonium
- 102783-61-9
- Ap5A pentaammonium
- P1,P5-Di(adenosine-5'-)pentaphosphate pentaammonium
- Endogenous Metabolite
- RyR2
- P2-type purinoceptors
- P1 purinoceptors
- P2X3
- IP3-sensitive intracellular stores
- I_KAch channels
- sheep cardiac ryanodine receptor
- heteromeric P2X2/3 purinoceptors
- ryanodine-sensitive intracellular stores
- P2X1
- Inhibitor
- inhibitor
- inhibit