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  6. Protease and Phosphatase Inhibitor Cocktail (EDTA-Free, 10× in ddH2O)

Protease and Phosphatase Inhibitor Cocktail (EDTA-Free, 10× in ddH2O) 

製品番号: HY-K0013
Manual COA SDS Technical Support
Cocktails

MCE Protease and Phosphatase Inhibitor Cocktail (EDTA-Free, 10× in ddH2O) protects protein from degradation by endogenous proteases released during protein extraction and purification.The 1 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

容量 価格（税別) 在庫状況 数量
無料サンプル (100 μL)   今すぐ申し込む
1 mL $50 在庫あり
10 mL $350 在庫あり
50 mL $1380 在庫あり
or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

顧客検証

WB

    Protease and Phosphatase Inhibitor Cocktail (EDTA-Free, 10× in ddH2O) purchased from MCE. Usage Cited in: Adv Sci (Weinh). 2024 May 8:e2305925.  [Abstract]

    Temporal dynamics of the ratio between Cry1 and housekeeping gene Actin expression levels following SS treatment in western blot analysis. Each dot stands for the relative protein expression of Cry1, which were fitted to the cosine function, and the p‐value was calculated.

    • 説明

    • 保管条件

    • プロトコル

    • 成分

    • ドキュメンテーション

    Description
    & Advantages

    MCE Protease and Phosphatase Inhibitor Cocktail (EDTA-Free, 10× in ddH2O) protects protein from degradation by endogenous proteases released during protein extraction and purification.

    This cocktail is AEBSF free and mass spectroscopy compatible�Cwater soluble. The protease inhibitors target serine proteases, aminopeptidase B, leucine aminopeptidase, cysteine proteases, metalloproteases. The phosphatase inhibitors target serine/threonine phosphatases, acid phosphatases, protein tyrosine.

    保管条件

    -20°C, 2 years
    プロトコル

    1.    This product can be applied in Western Blot analysis, Co-IP, pull-down, IF, IHC, kinase assay and etc.

    2.    Thaw at room temperature, add at 1:10 (v/v) dilution to 1 solution samples, mix throughly. It is recommended to prepare solutions of this product fresh when needed.

    3.    If necessary, 0.5 mM EDTA can be prepared and added to the mix by the ratio of 1:10 (v/v).
    成分
    Cat. No. Ingredient Target Package
    HY-K0013 Aprotinin Serine proteases 1 mL
    10 mL (1 mL × 10)
    50 mL (1 mL × 50)
    Bestatin Aminopeptidases
    E-64 Cysteine proteases
    Leupeptin hemisulfate Serine and Cysteine proteases
    β-Glycerophosphate Serine and Threonine phosphatases
    Sodium fluoride Serine and Threonine phosphatases
    Sodium orthovanadate Tyrosine phosphatases
    Sodium pyrophosphate decahydrate Serine and Threonine phosphatases
    ドキュメンテーション

    Manual (218 KB) SDS (224 KB)
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Protease InhibitorProtein Degradation InhibitorProtein inhibitor

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    製品名:
    Protease and Phosphatase Inhibitor Cocktail (EDTA-Free, 10× in ddH2O)
    製品番号:
    HY-K0013
    数量:
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