Protein A Agarose (Alkali-Tolerant) 4FF 

Protein A is a cell wall protein originally isolated from Staphylococcus aureus, known for its specific binding to the Fc region of mammalian immunoglobulin G (IgG) heavy chains. Native protein A contains five IgG-binding domains, along with several additional domains of unknown function. In contrast, recombinant protein A retains only the IgG-binding domains, significantly reducing nonspecific adsorption.

MCE Protein A Agarose (Alkali-Tolerant) 4FF is manufactured by covalently coupling recombinant protein A to a highly cross-linked agarose matrix. It features high binding capacity, excellent specificity, and strong ligand stability. The resin is alkali-tolerant and can withstand in-place cleaning with 0.5 M NaOH, making it suitable for repeated use. This product efficiently binds IgG from serum, ascites, culture supernatants, and other antibody-containing samples, enabling high-performance antibody purification.

Each 1 mL of total volume contains 0.5 mL of resin. Thoroughly resuspend the resin before use and mix well before aliquoting.

The specifications of the product correspond to the actual resin volume, with the resin content of 50%.

4°C, 5 years.

Do not dry or freeze.

1. Ensure the resin is thoroughly resuspended before use.

2. It is recommended to replace the storage buffer every 2-3 months to prevent microbial contamination due to ethanol evaporation.

3. To minimize protein degradation, protease inhibitor cocktails (MCE Cat. No. HY-K0010, HY-K0011) are highly recommended.

4. This product is for R&D use only, not for drug, household, or other uses.

5. For your safety and health, please wear a lab coat and disposable gloves to operate.