2. Stem Cell/Wnt TGF-beta/Smad
  3. Organoid TGF-β Receptor
  4. LDN193189 hydrochloride

LDN193189 hydrochloride  (Synonyms: DM-3189 hydrochloride)

製品番号: HY-12071C 純度: 98.62%
COA 取扱説明書 Technical Support

LDN193189 (DM-3189) hydrochloride is a potent selective BMP type I receptor (BMP I) inhibitor. LDN193189 efficiently inhibits transcriptional activity of the BMP type I receptors ALK2 and ALK3 with IC50 values of 5 nM and 30 nM, respectively. LDN193189 can be used for the research of bone morphogenetic protein signalling, such as fibrodysplasia ossificans progressiva.

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CAS 番号 : 1062368-62-0

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 88 在庫あり
Solution
10 mM * 1 mL in DMSO USD 88 在庫あり
Solid
5 mg $80 在庫あり
10 mg $105 在庫あり
25 mg $195 在庫あり
50 mg $290 在庫あり
100 mg $495 在庫あり
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500 mg   お問い合わせ  

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カスタマーレビュー

Based on 71 publication(s) in Google Scholar

Other Forms of LDN193189 hydrochloride:

LDN193189 hydrochloride 関連抗体

Top Publications Citing Use of Products

顧客検証

WB
IF
Cell Migration/Invasion Assay
IHC

    LDN193189 hydrochloride purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2022 Aug 19;13(1):424.  [Abstract]

    Phospho-Smad1/5/9, COL1A1 and RUNX2 were quantified in hBM-MSCs after 3 days of treatment with LDN193189 (100 nM; 3 days) by western blot analysis and normalized to GAPDH and tSmad1.

    LDN193189 hydrochloride purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2022 Aug 19;13(1):424.  [Abstract]

    LDN193189 (100 nM; 3 days). Representative images and quantification of immunofluorescence staining of COL1A1. Scale bars, 100 μm.

    LDN193189 hydrochloride purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2022 Aug 19;13(1):424.  [Abstract]

    LDN193189 (100 nM; 3 days). Representative images of ARS.

    LDN193189 hydrochloride purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2020 Jun 8;2020:4175613.  [Abstract]

    Western blot and grey analysis of CYP11a1, CYP19a1, BCL2, CALR, CHOP, caspase3, caspase12, PERK, and GRP78. qRT-PCR analyzed ATF4, caspase3, and GRP78 mRNAs. The relieving function of BMP4 on ER stress is diminished after blockade of BMP receptor I (BMPRI) with DM-3189.

    LDN193189 hydrochloride purchased from MedChemExpress. Usage Cited in: Cardiovasc Toxicol. 2019 Jun;19(3):264-275.  [Abstract]

    H9c2 cells are treated as following: control, DOX, DOX + rhBMP (Bmpr1a agonist) and DOX + LDN193189 (Bmpr1a inhibitor). Cells with red fluorescence indicate favorable MMP while green fluorescence indicates damage.

    TGF-β Receptor アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    ALK1 ALK2 ALK3 ALK4 ALK5 ALK6 ALK7 TGFβR2 ACVR2A ACVR2B BMP GDF15

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    • 純度とドキュメンテーション

    • 参考文献

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    製品説明

    LDN193189 (DM-3189) hydrochloride is a potent selective BMP type I receptor (BMP I) inhibitor. LDN193189 efficiently inhibits transcriptional activity of the BMP type I receptors ALK2 and ALK3 with IC50 values of 5 nM and 30 nM, respectively. LDN193189 can be used for the research of bone morphogenetic protein signalling, such as fibrodysplasia ossificans progressiva[1][2][3].

    IC50 & Target

    IC50: 5 nM (ALK2), 30 nM (ALK3)[1]
    体外実験

    LDN193189 hydrochloride efficiently inhibits transcriptional activity of the BMP type I receptors ALK2 and ALK3 with IC50 values of 5 nM and 30 nM, respectively[1].
    LDN193189 hydrochloride has weake effects on activin and the TGF-β type I receptors ALK4, ALK5 and ALK7 with IC50 values of ≥ 500 nM[1].
    LDN193189 hydrochloride binds ActRIIA with Kd value of 14 nM[2].
    LDN193189 hydrochloride (0.5 μM; 30 min) targets GDF8 induced Smad2/3 signaling and repression of myogenic transcription factors[2].
    LDN193189 hydrochloride (0.05, 0.5, 5 μM) efficiently inhibits GDF8 induced Smad3/4 reporter gene activity[2].
    LDN193189 hydrochloride (0-5 μM) rescues myogenesis in myoblasts treated with GDF8[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    LDN193189 hydrochloride 関連抗体
    体内実験

    LDN193189 (i.p.; 3 mg/kg; daily; for 35 days) hydrochloride might affect the interaction between breast cancer cells and the bone environment[3].
    LDN193189 (i.p.; 3 mg/kg; single) hydrochloride shows a reduction in ectopic ossification and functional impairment[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    442.94

    分子式

    C25H23ClN6
    CAS 番号
    Appearance

    Solid

    Color

    Yellow to orange

    SMILES

    [H]Cl.C1(C2=C3N=CC(C4=CC=C(N5CCNCC5)C=C4)=CN3N=C2)=CC=NC6=CC=CC=C16
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    H2O : 25 mg/mL (56.44 mM; Need ultrasonic)

    DMSO : 12.5 mg/mL (28.22 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.2576 mL 11.2882 mL 22.5764 mL
    5 mM 0.4515 mL 2.2576 mL 4.5153 mL
    10 mM 0.2258 mL 1.1288 mL 2.2576 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    一般には略語で表示されます：C1V1 = C2V2

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    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    純度とドキュメンテーション

    純度: 98.62%

    COA (255 KB) HNMR (287 KB) LCMS (96 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 2.2576 mL 11.2882 mL 22.5764 mL 56.4411 mL
    5 mM 0.4515 mL 2.2576 mL 4.5153 mL 11.2882 mL
    10 mM 0.2258 mL 1.1288 mL 2.2576 mL 5.6441 mL
    15 mM 0.1505 mL 0.7525 mL 1.5051 mL 3.7627 mL
    20 mM 0.1129 mL 0.5644 mL 1.1288 mL 2.8221 mL
    25 mM 0.0903 mL 0.4515 mL 0.9031 mL 2.2576 mL
    H2O 30 mM 0.0753 mL 0.3763 mL 0.7525 mL 1.8814 mL
    40 mM 0.0564 mL 0.2822 mL 0.5644 mL 1.4110 mL
    50 mM 0.0452 mL 0.2258 mL 0.4515 mL 1.1288 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    LDN193189 hydrochloride Related Classifications

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    一般には略語で表示されます：C1V1 = C2V2

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    LDN1931891062368-62-0DM-3189LDN 193189LDN-193189DM3189DM 3189OrganoidTGF-β ReceptorTransforming growth factor beta receptorsbiochemical reagentbiological materialorganic compoundlife scienceInhibitorinhibitorinhibit

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