Mecamylamine hydrochloride

Name: Mecamylamine hydrochloride
Brand: MedChemExpress
SKU: HY-B1395
Rating: 5.0 (5 reviews)

Cat. No.: HY-B1395 Purity: 98.0%: Data Sheet
COA

SDS
Handling Instructions
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Mecamylamine hydrochloride is an orally active, nonselective, noncompetitive nAChR antagonist. Mecamylamine hydrochloride is also a ganglionic blocker. Mecamylamine hydrochloride can across the blood-brain barrier. Mecamylamine hydrochloride can be used in the research of neuropsychiatric disorders, hypertension, antidepressant area.

For research use only. We do not sell to patients.

CAS No. : 826-39-1

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	Get quote
200 mg	Get quote

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Customer Review

Based on 5 publication(s) in Google Scholar

Other Forms of Mecamylamine hydrochloride:

Mecamylamine-d₃ hydrochloride Get quote
Mecamylamine hydrochloride-¹³C₄,¹⁵N Get quote
Mecamylamine Get quote
Mecamylamine hydrochloride (Standard) Get quote

5 Publications Citing Use of MCE Mecamylamine hydrochloride

Bio/Physico-chemical Assay

In Vivo Efficacy Study

: Mecamylamine hydrochloride purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2024 Jun;45(6):1160-1174. [Abstract]; Injection of Mecamylamine hydrochloride (MEC) (100 μM; 300 nL) into the SNr of mice reduced both mechanical and thermal thresholds on both hind paws.

: Mecamylamine hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Biosci. 2024 Dec 3;14(1):146. [Abstract]; Mecamylamine hydrochloride (Meca) (50uM; 500 nL; i.c.) effectively decreased the power of Den-induced more DA release events in parkinsonian mice.

: Mecamylamine hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Biosci. 2024 Dec 3;14(1):146. [Abstract]; Mecamylamine hydrochloride (Meca) (3 mg/kg; i.p.) blocked the CNO-induced (5 mg/kg) increase in DA concentration in 6-OHDA-lesioned ChAT-Cre mice.

: Mecamylamine hydrochloride purchased from MedChemExpress. Usage Cited in: bioRxiv. 2023 Jul 6.; Mecamylamine hydrochloride (MEC) (1 mg/kg i.p.) blocked nAChRs and abolished the ACh-induced spine formation in ChAT-Cre × YFP-H mice.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

nAChR^[1], histamine receptor^[2]

In Vitro

Mecamylamine hydrochloride (0.5-9 μM, bath administered) increases the firing frequency of identified 5-HT DRN (dorsal raphe nucleus) neurons^[1].
Mecamylamine hydrochloride (0.5-9 μM, bath administered) increases the glutamatergic and decreases the GABAergic input of 5-HT DRN neurons^[1].
Mecamylamine hydrochloride (1 mM, 5 min) blocks the histamine receptor and the histamine-induced contractions in helically cut strips of rabbit aorta^[2].
Mecamylamine hydrochloride (1-100 nM, 30 min) dose-dependently attenuates endothelial tube formation in HDMVECs^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[3]

Cell Line:	SCG neurons
Concentration:	10 μM
Incubation Time:	48 h
Result:	Reduced the nicotine-facilitated increase in ERK1/2.

In Vivo

Mecamylamine hydrochloride (subcutaneous pumps, 50 mg/kg/day, 2 days) inhibits Choroidal neovascularization (CNV) in CNV mice model^[4].
Mecamylamine hydrochloride (intraperitoneal injection, 0.5-1 mg/kg) has antidepressant-like effects in both the TST (tail suspension test) and FST (forced swim test) in C57BL/6J mice, which are dependent on both β2 and α7 subunits^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Choroidal neovascularization (CNV) mice model^[1]
Dosage:	50 mg/kg/day, 2 days
Administration:	Subcutaneous pumps implanted beneath the skin of the back, 200 μL and mean pumping rate of 0.5 μL/h.
Result:	Suppressed the development of CNV at Bruch’s membrane rupture sites in the absence of nicotine.

Animal Model:	C57BL/6J mice^[5]
Dosage:	0.5-1 mg/kg
Administration:	Intraperitoneal injection
Result:	Had no effect in β2 knockout mice and α7 knockout mice, but decreased immobility time in wildtype littermates in the FST.

Molecular Weight

203.75

Formula

C₁₁H₂₂ClN

CAS No.

826-39-1

Appearance

Solid

Color

White to off-white

SMILES

CC1(C)C(NC)(C)C2CCC1C2.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (490.80 mM; Need ultrasonic)

DMSO : 31.25 mg/mL (153.37 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.9080 mL	24.5399 mL	49.0798 mL
5 mM	0.9816 mL	4.9080 mL	9.8160 mL
10 mM	0.4908 mL	2.4540 mL	4.9080 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (10.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (10.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (10.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 98.0%

Select Batch:

Data Sheet (284 KB) SDS (479 KB)

COA (255 KB) HNMR (237 KB) MS (177 KB)

Handling Instructions (2659 KB)

References

[1]. Omar Hernández-González, et al. Mechanisms of stimulatory effects of mecamylamine on the dorsal raphe neurons. Brain Res Bull. 2020 Nov;164:289-298. [Content Brief]

[2]. C P Robinson, et al. The influence of mecamylamine on contractions induced by different agonists and on the role of calcium ions in the isolated rabbit aorta. J Pharmacol Exp Ther. 1976 Apr;197(1):57-65. [Content Brief]

[3]. Mahadevappa P Badanavalu, et al. Nicotine is neuroprotective to neonatal neurons of sympathetic ganglion in rat. Auton Neurosci. 2019 Jan;216:25-32. [Content Brief]

[4]. Katsuji Kiuchi, et al. Mecamylamine suppresses Basal and nicotine-stimulated choroidal neovascularization. Invest Ophthalmol Vis Sci. 2008 Apr;49(4):1705-11. [Content Brief]

[5]. Rabenstein RL, et al. The nicotinic antagonist mecamylamine has antidepressant-like effects in wild-type but not beta2- or alpha7-nicotinic acetylcholine receptor subunit knockout mice. Psychopharmacology (Berl). 2006 Dec;189(3):395-401. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	4.9080 mL	24.5399 mL	49.0798 mL	122.6994 mL
	5 mM	0.9816 mL	4.9080 mL	9.8160 mL	24.5399 mL
	10 mM	0.4908 mL	2.4540 mL	4.9080 mL	12.2699 mL
	15 mM	0.3272 mL	1.6360 mL	3.2720 mL	8.1800 mL
	20 mM	0.2454 mL	1.2270 mL	2.4540 mL	6.1350 mL
	25 mM	0.1963 mL	0.9816 mL	1.9632 mL	4.9080 mL
	30 mM	0.1636 mL	0.8180 mL	1.6360 mL	4.0900 mL
	40 mM	0.1227 mL	0.6135 mL	1.2270 mL	3.0675 mL
	50 mM	0.0982 mL	0.4908 mL	0.9816 mL	2.4540 mL
	60 mM	0.0818 mL	0.4090 mL	0.8180 mL	2.0450 mL
	80 mM	0.0613 mL	0.3067 mL	0.6135 mL	1.5337 mL
	100 mM	0.0491 mL	0.2454 mL	0.4908 mL	1.2270 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.