Cat. No.: HY-B1395

Mecamylamine hydrochloride

5 Cited Publications

Customer Review

Based on 5 publication(s) in Google Scholar

Mecamylamine hydrochloride is an orally active, nonselective, noncompetitive nAChR antagonist. Mecamylamine hydrochloride is also a ganglionic blocker. Mecamylamine hydrochloride can across the blood-brain barrier. Mecamylamine hydrochloride can be used in the research of neuropsychiatric disorders, hypertension, antidepressant area.

For research use only. We do not sell to patients.

Purity: 98.0%
CAS No.: 826-39-1
Formula: C₁₁H₂₂ClN
Molecular Weight:203.75

Storage:

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Mecamylamine hydrochloride Chemical Structure

CAS No.: 826-39-1

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (5)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target

nAChR^[1], histamine receptor^[2]

All Histamine Receptor Isoforms :

In Vitro

Mecamylamine hydrochloride (0.5-9 μM, bath administered) increases the firing frequency of identified 5-HT DRN (dorsal raphe nucleus) neurons^[1].
Mecamylamine hydrochloride (0.5-9 μM, bath administered) increases the glutamatergic and decreases the GABAergic input of 5-HT DRN neurons^[1].
Mecamylamine hydrochloride (1 mM, 5 min) blocks the histamine receptor and the histamine-induced contractions in helically cut strips of rabbit aorta^[2].
Mecamylamine hydrochloride (1-100 nM, 30 min) dose-dependently attenuates endothelial tube formation in HDMVECs^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[3]

Cell Line:

SCG neurons
Concentration:

10 μM
Incubation Time:

48 h
Result:

Reduced the nicotine-facilitated increase in ERK1/2.

In Vivo

Mecamylamine hydrochloride (subcutaneous pumps, 50 mg/kg/day, 2 days) inhibits Choroidal neovascularization (CNV) in CNV mice model^[4].
Mecamylamine hydrochloride (intraperitoneal injection, 0.5-1 mg/kg) has antidepressant-like effects in both the TST (tail suspension test) and FST (forced swim test) in C57BL/6J mice, which are dependent on both β2 and α7 subunits^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Choroidal neovascularization (CNV) mice model^[1]
Dosage:

50 mg/kg/day, 2 days
Administration:

Subcutaneous pumps implanted beneath the skin of the back, 200 μL and mean pumping rate of 0.5 μL/h.
Result:

Suppressed the development of CNV at Bruch’s membrane rupture sites in the absence of nicotine.

Animal Model:

C57BL/6J mice^[5]
Dosage:

0.5-1 mg/kg
Administration:

Intraperitoneal injection
Result:

Had no effect in β2 knockout mice and α7 knockout mice, but decreased immobility time in wildtype littermates in the FST.

Chemical Information

CAS No. 826-39-1
Appearance Solid
Molecular Weight 203.75
Formula C₁₁H₂₂ClN
Color White to off-white
SMILES

CC1(C)C(NC)(C)C2CCC1C2.[H]Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (5)

All Bio/Physico-chemical AssayIn Vivo Efficacy Study

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Journal Impact Factor

Nat Commun

Plasticity in ventral pallidal cholinergic neuron-derived circuits contributes to comorbid chronic pain-like and depression-like behaviour in male mice. [Abstract]

2023 Apr 17;14(1):2182. PMID: 37069246
Acta Pharmacol Sin

α4 nicotinic receptors on GABAergic neurons mediate a cholinergic analgesic circuit in the substantia nigra pars reticulata. [Abstract]

2024 Jun;45(6):1160-1174. PMID: 38438581

Mecamylamine hydrochloride purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2024 Jun;45(6):1160-1174. [Abstract]

Injection of Mecamylamine hydrochloride (MEC) (100 μM; 300 nL) into the SNr of mice reduced both mechanical and thermal thresholds on both hind paws.
Cell Biosci

Enhancing striatal acetylcholine facilitates dopamine release and striatal output in parkinsonian mice. [Abstract]

2024 Dec 3;14(1):146. PMID: 39627827

Mecamylamine hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Biosci. 2024 Dec 3;14(1):146. [Abstract]

Mecamylamine hydrochloride (Meca) (50uM; 500 nL; i.c.) effectively decreased the power of Den-induced more DA release events in parkinsonian mice.

Mecamylamine hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Biosci. 2024 Dec 3;14(1):146. [Abstract]

Mecamylamine hydrochloride (Meca) (3 mg/kg; i.p.) blocked the CNO-induced (5 mg/kg) increase in DA concentration in 6-OHDA-lesioned ChAT-Cre mice.
bioRxiv

2024 November 03.
bioRxiv

2023 Jul 6.

Mecamylamine hydrochloride purchased from MedChemExpress. Usage Cited in: bioRxiv. 2023 Jul 6.

Mecamylamine hydrochloride (MEC) (1 mg/kg i.p.) blocked nAChRs and abolished the ACh-induced spine formation in ChAT-Cre × YFP-H mice.

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (490.80 mM; Need ultrasonic)

DMSO : 31.25 mg/mL (153.37 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.9080 mL

24.5399 mL

49.0798 mL

5 mM

0.9816 mL

4.9080 mL

9.8160 mL

10 mM

0.4908 mL

2.4540 mL

4.9080 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (10.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (10.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (10.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (284 KB)
SDS (479 KB)
- English - EN (479 KB)
- Français - FR (479 KB)
- Deutsch - DE (479 KB)
- Norwegian - NO (479 KB)
- Español - ES (479 KB)
- Swedish - SV (479 KB)
- Italian - IT (479 KB)
- Korean - KR (479 KB)
- Portuguese - PT (479 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Omar Hernández-González, et al. Mechanisms of stimulatory effects of mecamylamine on the dorsal raphe neurons. Brain Res Bull. 2020 Nov;164:289-298. [Content Brief]

[2]. C P Robinson, et al. The influence of mecamylamine on contractions induced by different agonists and on the role of calcium ions in the isolated rabbit aorta. J Pharmacol Exp Ther. 1976 Apr;197(1):57-65. [Content Brief]

[3]. Mahadevappa P Badanavalu, et al. Nicotine is neuroprotective to neonatal neurons of sympathetic ganglion in rat. Auton Neurosci. 2019 Jan;216:25-32. [Content Brief]

[4]. Katsuji Kiuchi, et al. Mecamylamine suppresses Basal and nicotine-stimulated choroidal neovascularization. Invest Ophthalmol Vis Sci. 2008 Apr;49(4):1705-11. [Content Brief]

[5]. Rabenstein RL, et al. The nicotinic antagonist mecamylamine has antidepressant-like effects in wild-type but not beta2- or alpha7-nicotinic acetylcholine receptor subunit knockout mice. Psychopharmacology (Berl). 2006 Dec;189(3):395-401. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO / H₂O	1 mM	4.9080 mL	24.5399 mL	49.0798 mL	122.6994 mL
	5 mM	0.9816 mL	4.9080 mL	9.8160 mL	24.5399 mL
	10 mM	0.4908 mL	2.4540 mL	4.9080 mL	12.2699 mL
	15 mM	0.3272 mL	1.6360 mL	3.2720 mL	8.1800 mL
	20 mM	0.2454 mL	1.2270 mL	2.4540 mL	6.1350 mL
	25 mM	0.1963 mL	0.9816 mL	1.9632 mL	4.9080 mL
	30 mM	0.1636 mL	0.8180 mL	1.6360 mL	4.0900 mL
	40 mM	0.1227 mL	0.6135 mL	1.2270 mL	3.0675 mL
	50 mM	0.0982 mL	0.4908 mL	0.9816 mL	2.4540 mL
	60 mM	0.0818 mL	0.4090 mL	0.8180 mL	2.0450 mL
	80 mM	0.0613 mL	0.3067 mL	0.6135 mL	1.5337 mL
	100 mM	0.0491 mL	0.2454 mL	0.4908 mL	1.2270 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Mecamylamine hydrochloride

5 Cited Publications

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Mecamylamine hydrochloride

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Publications Citing Use of MedChemExpress (MCE) Mecamylamine hydrochloride

All Histamine Receptor Isoforms

Biological Activity

Chemical Information

Publications (5)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Mecamylamine hydrochloride Related Classifications

Cat. No.: HY-B1395