TCPTP Protein, Human (sf9)

TCPTP, a non-receptor tyrosine-specific phosphatase, critically regulates various kinases (INSR, EGFR, JAKs, Src), influencing hematopoiesis, inflammation, cell proliferation, and glucose homeostasis. In the immune system, TCPTP modulates T-cell receptor signaling, inhibiting cytokine pathways (IL2, interferon). It negatively regulates EGF-stimulated cell growth, PDGF receptor-beta signaling, and insulin receptor signaling, impacting glucose homeostasis and liver glucose production through the IL6 pathway. TCPTP's broad influence emphasizes its pivotal role in diverse cellular processes. TCPTP Protein, Human (sf9) is the recombinant human-derived TCPTP protein, expressed by Sf9 insect cells , with tag free. and with residual Gly-Pro in N-terminal (not related to functional changes) mutation. The total length of TCPTP Protein, Human (sf9) is 314 a.a., with molecular weight of ~37 kDa.

TCPTP, a non-receptor type tyrosine-specific phosphatase, acts as a crucial regulator by dephosphorylating various receptor and non-receptor protein tyrosine kinases, including INSR, EGFR, CSF1R, PDGFR, JAK1, JAK2, JAK3, Src family kinases, STAT1, STAT3, and STAT6, either in the nucleus or the cytoplasm. This phosphatase exerts negative control over diverse signaling pathways and biological processes such as hematopoiesis, inflammatory response, cell proliferation and differentiation, and glucose homeostasis. In the immune system, TCPTP plays a multifaceted role, influencing T-cell receptor signaling by dephosphorylating FYN and LCK to regulate T-cell differentiation and activation. Additionally, it negatively modulates cytokine-mediated signaling, including IL2, interferon, IL6, and IL4 pathways. Beyond immune regulation, TCPTP participates in anchorage-dependent, negative regulation of EGF-stimulated cell growth and PDGF receptor-beta signaling, thus influencing cell proliferation. Moreover, it plays a vital role in glucose homeostasis, negatively regulating the insulin receptor signaling pathway and controlling gluconeogenesis and liver glucose production through the IL6 signaling pathway. TCPTP's broad impact underscores its significance in diverse cellular processes, and it may also have DNA binding capabilities.

Human

Sf9 insect cells

Tag Free

P17706 (N-G&P, M1-N314)

5771  [NCBI]

Approximately 37 kDa

Greater than 95% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.