2. Metabolic Enzyme/Protease Apoptosis
  3. FXR Apoptosis
  4. Tauro-β-muricholic acid

Tauro-β-muricholic acid  (Synonyms: TβMCA)

製品番号: HY-N9933 純度: 99.91%
COA 取扱説明書 Technical Support

Tauro-β-muricholic acid (TβMCA) is an orally active trihydroxylated bile acid and a competitive, reversible FXR antagonist (IC50=40 μM). Tauro-β-muricholic acid inhibits bile acid-induced hepatocyte apoptosis by maintaining mitochondrial membrane potential, while simultaneously inhibiting intestinal FXR signaling, affecting bile acid synthesis, hepatic lipid metabolism, and insulin sensitivity. Accumulation of tauro-β-muricholic acid disrupts metabolic homeostasis, promoting cancer stem cell proliferation and tumor progression. The mechanisms of tauro-β-muricholic acid involve two aspects: first, inhibiting the translocation of the pro-apoptotic protein Bax to mitochondria and maintaining mitochondrial membrane potential (MMP); and second, blocking the FXR signaling pathway to regulate bile acid metabolism, reduce serum ceramide production, and downregulate the hepatic SREBP1C/CIDEA pathway. Tauro-β-muricholic acid possesses anti-hepatocyte apoptosis, bile acid homeostasis regulation, and liver fat accumulation reduction properties, and also functions as a biomarker, making it useful in the study of diseases such as bile acid metabolism disorders, non-alcoholic fatty liver disease, colorectal cancer, and liver fibrosis.

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Tauro-β-muricholic acid

Tauro-β-muricholic acid 構造式

CAS 番号 : 25696-60-0

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 581 在庫あり
Solution
10 mM * 1 mL in DMSO USD 581 在庫あり
Solid
1 mg $170 在庫あり
5 mg $512 在庫あり
10 mg $820 在庫あり
25 mg $1640 在庫あり
50 mg $2625 在庫あり
100 mg $4200 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

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Based on 4 publication(s) in Google Scholar

Other Forms of Tauro-β-muricholic acid:

Tauro-β-muricholic acid 関連抗体

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製品説明

Tauro-β-muricholic acid (TβMCA) is an orally active trihydroxylated bile acid and a competitive, reversible FXR antagonist (IC50=40 μM). Tauro-β-muricholic acid inhibits bile acid-induced hepatocyte apoptosis by maintaining mitochondrial membrane potential, while simultaneously inhibiting intestinal FXR signaling, affecting bile acid synthesis, hepatic lipid metabolism, and insulin sensitivity. Accumulation of tauro-β-muricholic acid disrupts metabolic homeostasis, promoting cancer stem cell proliferation and tumor progression. The mechanisms of tauro-β-muricholic acid involve two aspects: first, inhibiting the translocation of the pro-apoptotic protein Bax to mitochondria and maintaining mitochondrial membrane potential (MMP); and second, blocking the FXR signaling pathway to regulate bile acid metabolism, reduce serum ceramide production, and downregulate the hepatic SREBP1C/CIDEA pathway. Tauro-β-muricholic acid possesses anti-hepatocyte apoptosis, bile acid homeostasis regulation, and liver fat accumulation reduction properties, and also functions as a biomarker, making it useful in the study of diseases such as bile acid metabolism disorders, non-alcoholic fatty liver disease, colorectal cancer, and liver fibrosis[1][2][3][4].

IC50 & Target

40 μM (FXR)[1]
体外実験

Co-incubation of tauro-β-muricholic acid (25 μM; 4 h) with GCDCA reduced apoptosis in human Ntcp-HepG2 cells, and co-incubation with palmitic acid restored the GCDCA-induced disruption of mitochondrial membrane potential (MMP). Both treatments also inhibited the translocation of the pro-apoptotic protein Bax to the mitochondria[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Tauro-β-muricholic acid 関連抗体

Apoptosis Analysis[1]

Cell Line: Human Ntcp-transfected HepG2 cells, Primary rat hepatocytes, Primary mouse hepatocytes
Concentration: 25 μmol/L (Ntcp-HepG2 cells, primary rat hepatocytes), 100 μmol/L (primary mouse hepatocytes)
Incubation Time: 24 h
Result: When co-incubated with glycochenodeoxycholic acid (GCDCA), Tauro-β-muricholic acid reduced GCDCA-induced hepatocellular apoptosis to 49% in Ntcp-HepG2 cells, and significantly decreased apoptosis in primary rat and mouse hepatocytes after 4 hours of treatment; nuclear fragmentation induced by GCDCA was also inhibited as visualized by Hoechst 33342 staining.
体内実験

Tauro-β-muricholic acid (400 mg/kg, in combination with TCA; gavage; twice, 12 hours apart; single dose) inhibited TCA-induced ileal Fgf15 and Shp gene expression, exerting an FXR antagonistic effect, and simultaneously inhibited intestinal FXR signaling, reduced serum ceramides, and improved hepatic steatosis in high-fat diet-induced non-alcoholic fatty liver disease (NAFLD) model mice [2][4]. Tauro-β-muricholic acid (oral antibiotic, 200 mg/kg/day; once daily; 3 days) increased TbMCA levels in the gallbladder of mice, inhibited ileal Fgf15 expression, upregulated hepatic Cyp7a1 expression, and altered bile acid composition [2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Swiss Webster mice (male, 9-16 weeks old), conventional raised (CONV-R) and germ-free (GF) mice; C57BL/6 mice (male, 9-16 weeks old), Fxr-deficient mice (backcrossed 8 generations on C57BL/6 background), CONV-R and GF mice [2]
Dosage: Combined with TCA, 400 mg/kg, 200 mg/kg/day
Administration: Oral administration
Result: Significantly reduced the TCA-induced expression of Fgf15 and Shp in the ileum of GF C57BL/6 mice. Antibiotic treatment increased the levels of TbMCA in the gallbladder of CONV-R C57BL/6 mice, suppressed ileal Fgf15 expression, and increased hepatic Cyp7a1 expression. GF mice had higher levels of TbMCA and a larger bile acid pool compared with CONV-R mice, and TbMCA functioned as a natural FXR antagonist to inhibit bile acid synthesis.
分子量

515.70

分子式

C26H45NO7S
CAS 番号
Appearance

Solid

Color

White to off-white

SMILES

C[C@@]12[C@]3([H])[C@]([C@H]([C@H]([C@]1([H])C[C@@H](CC2)O)O)O)([H])[C@@]4([H])[C@](CC3)([C@@](CC4)([H])[C@H](C)CCC(NCCS(=O)(O)=O)=O)C
Structure Classification
Initial Source

rat
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
溶剤 & 溶解度
体外: 

DMSO : 25 mg/mL (48.48 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9391 mL 9.6956 mL 19.3911 mL
5 mM 0.3878 mL 1.9391 mL 3.8782 mL
10 mM 0.1939 mL 0.9696 mL 1.9391 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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一般には略語で表示されます：C1V1 = C2V2

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純度とドキュメンテーション
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.9391 mL 9.6956 mL 19.3911 mL 48.4778 mL
5 mM 0.3878 mL 1.9391 mL 3.8782 mL 9.6956 mL
10 mM 0.1939 mL 0.9696 mL 1.9391 mL 4.8478 mL
15 mM 0.1293 mL 0.6464 mL 1.2927 mL 3.2319 mL
20 mM 0.0970 mL 0.4848 mL 0.9696 mL 2.4239 mL
25 mM 0.0776 mL 0.3878 mL 0.7756 mL 1.9391 mL
30 mM 0.0646 mL 0.3232 mL 0.6464 mL 1.6159 mL
40 mM 0.0485 mL 0.2424 mL 0.4848 mL 1.2119 mL
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Tauro-β-muricholic acid Related Classifications

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The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
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    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tauro-β-muricholic acid25696-60-0TβMCAFXRApoptosisNR1H4bile acidantiapoptoticmitochondrial membrane potentialInhibitorinhibitorinhibit

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