1. Cell Cycle/DNA Damage
    Cytoskeleton
  2. Microtubule/Tubulin

Vincristine sulfate (Synonyms: Leurocristine sulfate; 22-Oxovincaleukoblastine sulfate)

Cat. No.: HY-N0488 Purity: 99.51%
Data Sheet SDS Handling Instructions

Vincristine (sulfate) is an inhibitor of polymerization of microtubules by binding to tubulin with IC50 of 32 μM in a cell-free assay.

For research use only. We do not sell to patients.
Vincristine sulfate Chemical Structure

Vincristine sulfate Chemical Structure

CAS No. : 2068-78-2

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Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO $55 In-stock
10 mg $50 In-stock
50 mg $110 In-stock
100 mg $180 In-stock
200 mg $330 In-stock
500 mg   Get quote  
1 g   Get quote  

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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Vincristine (sulfate) is an inhibitor of polymerization of microtubules by binding to tubulin with IC50 of 32 μM in a cell-free assay.

In Vitro

Vincristine inhibits net addition of tubulin dimers at assembly ends of steady-state microtubules with Ki of 85 nM[1]. Vincristine stabilizes the spindle apparatus resulting in failure of the chromosomes to segregate leading to metaphase arrest and inhibition of mitosis at low concentrations. At higher concentrations, Vincristine may disrupt and induce total depolymerization of microtubules[2]. Vincristine induces apoptosis in tumor cells and inhibits SH-SY5Y cell proliferation with IC50 of 0.1 μM. Vincristine induces mitotic arrest and promots the expression of caspase-3 and -9 and cyclin B, while decreasing the expression of cyclin D[3]. Vincristine induced neurotoxicity is caused by interference with microtubule function, which results in blockage of axonal transport and thus in axonal degeneration[4]

In Vivo

Vincristine (3 mg/kg, i.p.) induces mean growth delay of > 120 and > 52 day, and repopulates fractions of 0.06% and 5%, administrated in mice bearing bilateral subcutaneous xenografts Rh12 or Rh18, respectively[5]

Clinical Trial
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References
Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg
1 mM 1.0834 mL 5.4169 mL 10.8338 mL
5 mM 0.2167 mL 1.0834 mL 2.1668 mL
10 mM 0.1083 mL 0.5417 mL 1.0834 mL
Please refer to the solubility information to select the appropriate solvent.
Cell Assay
[1]

Cells are plated in 2 mL of medium in 35 mm plates at a concentration of about 5×104 cells/mL and grow for 24 h at 37°C in an atmosphere of 5% CO2 and 95% air. Then medium is replaced with fresh medium lacking or containing 4 nM drug and proliferation is continued for 3 days. Cell counts are done each day in a Coulter Counter after detaching the cells with trypsin and EDTA. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

923.04

Formula

C₄₆H₅₈N₄O₁₄S

CAS No.

2068-78-2

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

10 mM in DMSO

Vincristine solution (dissolved with acetone) is mixed with PLGA solution[6].

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

References

Purity: 99.51%

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Product Name:
Vincristine sulfate
Cat. No.:
HY-N0488
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