1. Cell Cycle/DNA Damage
    Cytoskeleton
  2. Microtubule/Tubulin
  3. Vincristine sulfate

Vincristine sulfate (Synonyms: Leurocristine sulfate; 22-Oxovincaleukoblastine sulfate)

Cat. No.: HY-N0488 Purity: 99.66%
Handling Instructions

Vincristine sulfate is an antitumor vinca alkaloid which inhibits microtubule formation in mitotic spindle, resulting in an arrest of dividing cells at the metaphase stage. It binds to microtubule with a Ki of 85 nM.

For research use only. We do not sell to patients.

Vincristine sulfate Chemical Structure

Vincristine sulfate Chemical Structure

CAS No. : 2068-78-2

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50 mg USD 132 In-stock
Estimated Time of Arrival: December 31
100 mg USD 216 In-stock
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200 mg USD 396 In-stock
Estimated Time of Arrival: December 31
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Top Publications Citing Use of Products

    Vincristine sulfate purchased from MCE. Usage Cited in: Int J Biol Macromol. 2019 Jan 30;128:574-582.

    SGC7901/DDP or SGC7901/VCR cells are transfectedwith si-Con or si-ERK, and transfection efficiency is evaluated by western blot assay at 48 h post transfection.
    • Biological Activity

    • Protocol

    • Technical Information

    • Purity & Documentation

    • References

    Description

    Vincristine sulfate is an antitumor vinca alkaloid which inhibits microtubule formation in mitotic spindle, resulting in an arrest of dividing cells at the metaphase stage. It binds to microtubule with a Ki of 85 nM.

    In Vitro

    Vincristine inhibits net addition of tubulin dimers at assembly ends of steady-state microtubules with Ki of 85 nM[1]. Vincristine stabilizes the spindle apparatus resulting in failure of the chromosomes to segregate leading to metaphase arrest and inhibition of mitosis at low concentrations. At higher concentrations, Vincristine may disrupt and induce total depolymerization of microtubules[2]. Vincristine induces apoptosis in tumor cells and inhibits SH-SY5Y cell proliferation with IC50 of 0.1 μM. Vincristine induces mitotic arrest and promots the expression of caspase-3 and -9 and cyclin B, while decreasing the expression of cyclin D[3]. Vincristine induced neurotoxicity is caused by interference with microtubule function, which results in blockage of axonal transport and thus in axonal degeneration[4].

    In Vivo

    Vincristine (3 mg/kg, i.p.) induces mean growth delay of > 120 and > 52 day, and repopulates fractions of 0.06% and 5%, administrated in mice bearing bilateral subcutaneous xenografts Rh12 or Rh18, respectively[5].

    Clinical Trial
    Storage

    4°C, protect from light

    Solvent & Solubility
    In Vitro: 

    DMSO : 82.5 mg/mL (89.38 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.0834 mL 5.4169 mL 10.8338 mL
    5 mM 0.2167 mL 1.0834 mL 2.1668 mL
    10 mM 0.1083 mL 0.5417 mL 1.0834 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.75 mg/mL (2.98 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.75 mg/mL (2.98 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.75 mg/mL (2.98 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References
    Cell Assay
    [1]

    Cells are plated in 2 mL of medium in 35 mm plates at a concentration of about 5×104 cells/mL and grow for 24 h at 37°C in an atmosphere of 5% CO2 and 95% air. Then medium is replaced with fresh medium lacking or containing 4 nM drug and proliferation is continued for 3 days. Cell counts are done each day in a Coulter Counter after detaching the cells with trypsin and EDTA.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
    Molecular Weight

    923.04

    Formula

    C₄₆H₅₈N₄O₁₄S

    CAS No.

    2068-78-2

    SMILES

    CC[[email protected]@]1(C=CCN2CC3)[[email protected]@]2([H])[[email protected]@]3(C4=CC([[email protected]](C5=C6C7=CC=CC=C7N5)(C[[email protected]](C[[email protected]](CC)(O)C8)([H])C[[email protected]@]8CC6)C(OC)=O)=C(OC)C=C4N9C=O)[[email protected]]9([H])[[email protected]](C(OC)=O)(O)[[email protected]@H]1OC(C)=O.O=S(O)(O)=O

    Shipping

    Room temperature in continental US; may vary elsewhere

    Purity: 99.66%

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    Product Name:
    Vincristine sulfate
    Cat. No.:
    HY-N0488
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    Vincristine sulfate

    Cat. No.: HY-N0488