α-Methylserotonin

Cat. No.: HY-145463: Ficha de datos
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α-Methylserotonin is a potent and selective agonist of 5-HT₂ receptor. α-Methylserotonin is an analogue of serotonin formed in vivo from α-methyltryptophan. α-Methylserotonin mediates the lymphatic smooth muscle contraction and prevents the up-regulation of serotonin-receptor-mediated phosphoinositide hydrolysis.

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α-Methylserotonin Estructura química

No. CAS : 304-52-9

Tamaño	Precio	Stock
5 mg		Pide un presupuesto y un plazo de entrega
10 mg		Pide un presupuesto y un plazo de entrega
25 mg		Pide un presupuesto y un plazo de entrega

* Seleccione Cantidad antes de agregar artículos.

This product is a controlled substance and not for sale in your territory.

Other In-stock Forms of α-Methylserotonin:

α-Methylserotonin maleate

Other Forms of α-Methylserotonin:

α-Methylserotonin maleate In-stock

Recomendaciones destacadas

•Related Small Molecules:

•Related Proteins:

Ver todos los productos específicos de isoformas 5-HT Receptor:

Ver todas las isoformas

5-HT Receptor 5-HT₁ Receptor 5-HT₂ Receptor 5-HT₃ Receptor 5-HT₄ Receptor 5-HT₅ Receptor 5-HT₆ Receptor 5-HT₇ Receptor

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Referencias
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Descripciòn

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	EC₅₀	378 nM Compound: 2b	Tested for functional response on CHO cells expressing cloned human 5-hydroxytryptamine 1A receptor Tested for functional response on CHO cells expressing cloned human 5-hydroxytryptamine 1A receptor	[PMID: 12954071]

Peso molecular

190.24

Fòrmula

C₁₁H₁₄N₂O

No. CAS

304-52-9

Appearance

Solid

Color

Brown to black

SMILES

OC1=CC2=C(NC=C2CC(C)N)C=C1

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvente y solubilidad

In Vitro:

Methanol : 62.5 mg/mL (328.53 mM; ultrasonic and warming and heat to 60°C)

DMSO : 50 mg/mL (262.83 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.2565 mL	26.2826 mL	52.5652 mL
5 mM	1.0513 mL	5.2565 mL	10.5130 mL
10 mM	0.5257 mL	2.6283 mL	5.2565 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculadora de molaridad
Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (13.14 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (13.14 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Pureza y Documentación

Ficha de datos (273 KB) SDS (252 KB) Instrucciones de manejo (2659 KB)

Referencias

[1]. Dobbins DE. Receptor mechanisms of serotonin-induced prenodal lymphatic constriction in the canine forelimb. Am J Physiol. 1998 Feb;274(2):H650-4. [Content Brief]

[2]. Brumley MR, et al. The serotonergic agonists quipazine, CGS-12066A, and alpha-methylserotonin alter motor activity and induce hindlimb stepping in the intact and spinal rat fetus. Behav Neurosci. 2005 Jun;119(3):821-33. [Content Brief]

[3]. Edwards E, et al. (+/-)-1-(2,5-Dimethoxy-4-iodophenyl)-2-aminopropane(DOI) and alpha-methyl-5-HT: 5-HT2 receptor agonistic action on phosphatidylinositol metabolism in the rat fronto-cingulate and entorhinal cortex. Neuropharmacology. 1992 Jul;31(7):615-21. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / Methanol	1 mM	5.2565 mL	26.2826 mL	52.5652 mL	131.4129 mL
	5 mM	1.0513 mL	5.2565 mL	10.5130 mL	26.2826 mL
	10 mM	0.5257 mL	2.6283 mL	5.2565 mL	13.1413 mL
	15 mM	0.3504 mL	1.7522 mL	3.5043 mL	8.7609 mL
	20 mM	0.2628 mL	1.3141 mL	2.6283 mL	6.5706 mL
	25 mM	0.2103 mL	1.0513 mL	2.1026 mL	5.2565 mL
	30 mM	0.1752 mL	0.8761 mL	1.7522 mL	4.3804 mL
	40 mM	0.1314 mL	0.6571 mL	1.3141 mL	3.2853 mL
	50 mM	0.1051 mL	0.5257 mL	1.0513 mL	2.6283 mL
	60 mM	0.0876 mL	0.4380 mL	0.8761 mL	2.1902 mL
	80 mM	0.0657 mL	0.3285 mL	0.6571 mL	1.6427 mL
	100 mM	0.0526 mL	0.2628 mL	0.5257 mL	1.3141 mL