2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. ATP disodium salt

ATP disodium salt  (Synonyms: Adenosine 5'-triphosphate disodium salt; Disodium adenosine triphosphate)

Cat. No.: HY-B0345A Purity: 99.95%
COA
SDS
Handling Instructions Technical Support

ATP disodium salt (Adenosine 5'-triphosphate disodium salt) is a central component of energy storage and metabolism in vivo, provides the metabolic energy to drive metabolic pumps and serves as a coenzyme in cells. ATP disodium salt is an important endogenous signaling molecule in immunity and inflammation.

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CAS No. : 987-65-5

사이즈 가격 재고 수량
500 mg 해외재고보유
1 g 해외재고보유
5 g 해외재고보유
10 g   견적 받기  
50 g   견적 받기  

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고객리뷰

Based on 149 publication(s) in Google Scholar

Other Forms of ATP disodium salt:

ATP disodium salt Related Antibodies

Top Publications Citing Use of Products

149 Publications Citing Use of MCE ATP disodium salt

WB
Cell Proliferation/Viability Assay
RT-PCR

    ATP disodium salt purchased from MedChemExpress. Usage Cited in: Immunity. 2024 Mar 12;57(3):495-512.e11.  [Abstract]

    Representative western blots and analyses of the following proteins in BV2 cells treated with vehicle (Con), LPS (200 ng/ml, 3 h), LPS + ATP (3 mM, 30 min), or LPS + ATP-siNKAα1.

    ATP disodium salt purchased from MedChemExpress. Usage Cited in: Transl Res. 2023 May:255:1-13.

    LPS/ATP treatment significantly promotes expression of NLRP3 and production of N-GSDMD in HAEC. HAEC are pretreated with LPS (500ng/ml) for 5 h and then stimulated with ATP (5 mM) for 1 h.

    ATP disodium salt purchased from MedChemExpress. Usage Cited in: Cell Death Differ. 2022 Feb;29(2):439-450.  [Abstract]

    Primary BMDM cells were isolated from WT and Trim21-/- C57BL/6 mice. Cells were primed with 1 μg/mL LPS for 2 h followed by 5 mM ATP or 20 μM Nig or 30 μM flagellin for 30 min. Cell viability are determined by the Cell Counting Kit-8.

    ATP disodium salt purchased from MedChemExpress. Usage Cited in: Crit Care. 2021 Oct 12;25(1):356.  [Abstract]

    Stimulation of Raw264.7 macrophages with PBS/PBS-Exo/TNF-Exo for 24 h and treatment with PBS, 5 mM ATP or 20 mM nigericin for 2 h. Cleavage of GSDMD by western blot. GSDMD-FL: full-length GSDMD, GSDMD-N: N-terminal cleavage products of GSDMD.

    ATP disodium salt purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 Feb 18;11(2):132.   [Abstract]

    KCs were stimulated with 100 ng/mL LPS for 4 hours and subsequently treated them with 5 mM ATP for 2 hours to induce pyroptosis. The RNA level of lnc-Lfar1 was determined by qRT-PCR.

    ATP disodium salt purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 Feb 18;11(2):132.   [Abstract]

    KCs were transfected with lnc-Lfar1 siRNA for 24 h, followed by stimulation with 100 ng/ml LPS for 4 h and subsequently treated with 5 mM ATP or 100 ng/ml for 2 h to induce pyroptosis. a, b The protein level of NLRP3, Caspase1 and GSDMD was determined by western blot. GAPDH was used as an internal control.

    View All Endogenous Metabolite Isoform Specific Products:

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    Human Endogenous Metabolite Microbial Metabolite

    • Biological Activity

    • 순도&문서

    • References

    • 고객리뷰

    제품 설명

    ATP disodium salt (Adenosine 5'-triphosphate disodium salt) is a central component of energy storage and metabolism in vivo, provides the metabolic energy to drive metabolic pumps and serves as a coenzyme in cells. ATP disodium salt is an important endogenous signaling molecule in immunity and inflammation[1][2].

    IC50 & Target

    Human Endogenous Metabolite

     

    In Vitro

    ATP disodium salt (5 mM; 1 hour) co-treatment with LPS (1 μg/ml) has a synergistic effect on the activation of the NLRP3 inflammasome in HGFs[3].
    ATP disodium salt (2 mM; 0.5-24 hours) induces secretion of interleukin 1β, KC and MIP-2 from bone marrow derived macrophages (BMDMs) in vitro in a caspase-1 activation-dependent manner[4].
    ATP disodium salt stimulats cytokine and chemokine secretion and inflammasome activation directly and indirectly induces in vitro neutrophil chemotaxis[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    ATP disodium salt Related Antibodies
    In Vivo

    ATP disodium salt (50 mg/kg; i.p.) protects mice against bacterial infection in vivo[4].
    ATP disodium salt induces the secretion of IL1β, KC and MIP-2 and neutrophils recruitment in vivo[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Four-week-old Kunming mice (18-22 g)[4]
    Dosage: 50 mg/kg
    Administration: Intraperitoneal injection, before bacterial (E. coli) challenge
    Result: Protected mice from bacterial infection.
    분자량

    551.14

    화학식

    C10H14N5Na2O13P3
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O[C@H]1[C@@H](O)[C@H](N2C(N=CN=C3N)=C3N=C2)O[C@@H]1COP(OP(OP(O)([O-])=O)([O-])=O)(O)=O.[2Na+]
    Structure Classification
    Initial Source

    Widespread
    선적

    Room temperature in continental US; may vary elsewhere.
    보관

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    용액&용해도
    In Vitro: 

    H2O : 100 mg/mL (181.44 mM; Need ultrasonic)

    DMSO : < 1 mg/mL (insoluble or slightly soluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.8144 mL 9.0721 mL 18.1442 mL
    5 mM 0.3629 mL 1.8144 mL 3.6288 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • 몰농도 계산기

    • 농도 희석 계산기

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (181.44 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    순도&문서

    Purity: 99.95%

    SDS (393 KB)

    COA (251 KB) HNMR (120 KB) RP-HPLC (205 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 1.8144 mL 9.0721 mL 18.1442 mL 45.3605 mL
    5 mM 0.3629 mL 1.8144 mL 3.6288 mL 9.0721 mL
    10 mM 0.1814 mL 0.9072 mL 1.8144 mL 4.5361 mL
    15 mM 0.1210 mL 0.6048 mL 1.2096 mL 3.0240 mL
    20 mM 0.0907 mL 0.4536 mL 0.9072 mL 2.2680 mL
    25 mM 0.0726 mL 0.3629 mL 0.7258 mL 1.8144 mL
    30 mM 0.0605 mL 0.3024 mL 0.6048 mL 1.5120 mL
    40 mM 0.0454 mL 0.2268 mL 0.4536 mL 1.1340 mL
    50 mM 0.0363 mL 0.1814 mL 0.3629 mL 0.9072 mL
    60 mM 0.0302 mL 0.1512 mL 0.3024 mL 0.7560 mL
    80 mM 0.0227 mL 0.1134 mL 0.2268 mL 0.5670 mL
    100 mM 0.0181 mL 0.0907 mL 0.1814 mL 0.4536 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    ATP disodium salt Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    ATP disodium987-65-5Adenosine 5'-triphosphate disodium Disodium adenosine triphosphateEndogenous MetaboliteenergystoragemetabolismimmunityinflammationNLRP3inflammasomecytokineInhibitorinhibitorinhibit

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    상품명:
    ATP disodium salt
    Cat. No.:
    HY-B0345A
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